Smart mutational exploration of the CalB lipase active site using a combination of virtual screening and iterative saturation mutagenesis

• Main Authors: Lopez de los Santos, Yossef, Brault, Guillaume, Doucet, Nicolas
• Format: Other/Unknown Material
• Language: unknown
• Subjects: -; Tive; Antarc*; Antarctica
• Online Access: https://espace.inrs.ca/id/eprint/5794/; http://www.profs.inrs.ca/ndoucet/PEC2016_Final_Program.pdf.

One of the key elements for proper directed evolution of protei ns is the cyclic use of mutagenesis and selection processes, giving rise to libraries containing mi llions of mutants. However, analyzing such an important number of mutants is not a trivial task, as the identification of active variant...