Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels.
We determined the intracellular compartmentalization of the trace metals Ag, As, Cd, Ni, Pb and Tl in the livers of yellow eels collected from the Saint Lawrence River system in Canada (Anguilla rostrata) and in the area of the Gironde estuary in France (Anguilla anguilla). Differential centrifugati...
Published in: | Aquatic Toxicology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2015
|
Subjects: | |
Online Access: | https://espace.inrs.ca/id/eprint/3926/ https://doi.org/10.1016/j.aquatox.2015.01.011 |
id |
ftinrsquebec:oai:espace.inrs.ca:3926 |
---|---|
record_format |
openpolar |
spelling |
ftinrsquebec:oai:espace.inrs.ca:3926 2023-05-15T13:27:06+02:00 Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. Rosabal, Maikel Pierron, Fabien Couture, Patrice Baudrimont, Magalie Hare, Landis Campbell, Peter G. C. 2015 https://espace.inrs.ca/id/eprint/3926/ https://doi.org/10.1016/j.aquatox.2015.01.011 unknown Rosabal, Maikel, Pierron, Fabien, Couture, Patrice orcid:0000-0002-1944-5136 , Baudrimont, Magalie, Hare, Landis et Campbell, Peter G. C. (2015). Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. Aquatic Toxicology , vol. 160 . p. 128-141. DOI:10.1016/j.aquatox.2015.01.011 <https://doi.org/10.1016/j.aquatox.2015.01.011>. doi:10.1016/j.aquatox.2015.01.011 subcellular partitioning anguilla eels metal toxicity detoxification Article Évalué par les pairs 2015 ftinrsquebec https://doi.org/10.1016/j.aquatox.2015.01.011 2023-02-10T11:42:46Z We determined the intracellular compartmentalization of the trace metals Ag, As, Cd, Ni, Pb and Tl in the livers of yellow eels collected from the Saint Lawrence River system in Canada (Anguilla rostrata) and in the area of the Gironde estuary in France (Anguilla anguilla). Differential centrifugation, NaOH digestion and thermal shock were used to separate eel livers into putative “sensitive” fractions (heat-denatured proteins, mitochondria and microsomes + lysosomes) and detoxified metal fractions (heat-stable peptides/proteins and granules). The cytosolic heat-stable fraction (HSP) was consistently involved in the detoxification of all trace metals. In addition, granule-like structures played a complementary role in the detoxification of Ni, Pb and Tl in both eel species. However, these detoxification mechanisms were not completely effective because increasing trace metal concentrations in whole livers were accompanied by significant increases in the concentrations of most trace metals in “sensitive” subcellular fractions, that is, mitochondria, heat-denatured cytosolic proteins and microsomes + lysosomes. Among these “sensitive” fractions, mitochondria were the major binding sites for As, Cd, Pb and Tl. This accumulation of non-essential metals in “sensitive” fractions likely represents a health risk for eels inhabiting the Saint Lawrence and Gironde environments. Article in Journal/Newspaper Anguilla anguilla Institut national de la recherche scientifique, Québec: Espace INRS Canada Lawrence River ENVELOPE(-115.002,-115.002,58.384,58.384) Aquatic Toxicology 160 128 141 |
institution |
Open Polar |
collection |
Institut national de la recherche scientifique, Québec: Espace INRS |
op_collection_id |
ftinrsquebec |
language |
unknown |
topic |
subcellular partitioning anguilla eels metal toxicity detoxification |
spellingShingle |
subcellular partitioning anguilla eels metal toxicity detoxification Rosabal, Maikel Pierron, Fabien Couture, Patrice Baudrimont, Magalie Hare, Landis Campbell, Peter G. C. Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. |
topic_facet |
subcellular partitioning anguilla eels metal toxicity detoxification |
description |
We determined the intracellular compartmentalization of the trace metals Ag, As, Cd, Ni, Pb and Tl in the livers of yellow eels collected from the Saint Lawrence River system in Canada (Anguilla rostrata) and in the area of the Gironde estuary in France (Anguilla anguilla). Differential centrifugation, NaOH digestion and thermal shock were used to separate eel livers into putative “sensitive” fractions (heat-denatured proteins, mitochondria and microsomes + lysosomes) and detoxified metal fractions (heat-stable peptides/proteins and granules). The cytosolic heat-stable fraction (HSP) was consistently involved in the detoxification of all trace metals. In addition, granule-like structures played a complementary role in the detoxification of Ni, Pb and Tl in both eel species. However, these detoxification mechanisms were not completely effective because increasing trace metal concentrations in whole livers were accompanied by significant increases in the concentrations of most trace metals in “sensitive” subcellular fractions, that is, mitochondria, heat-denatured cytosolic proteins and microsomes + lysosomes. Among these “sensitive” fractions, mitochondria were the major binding sites for As, Cd, Pb and Tl. This accumulation of non-essential metals in “sensitive” fractions likely represents a health risk for eels inhabiting the Saint Lawrence and Gironde environments. |
format |
Article in Journal/Newspaper |
author |
Rosabal, Maikel Pierron, Fabien Couture, Patrice Baudrimont, Magalie Hare, Landis Campbell, Peter G. C. |
author_facet |
Rosabal, Maikel Pierron, Fabien Couture, Patrice Baudrimont, Magalie Hare, Landis Campbell, Peter G. C. |
author_sort |
Rosabal, Maikel |
title |
Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. |
title_short |
Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. |
title_full |
Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. |
title_fullStr |
Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. |
title_full_unstemmed |
Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. |
title_sort |
subcellular partitioning of non-essential trace metals (ag, as, cd, ni, pb, tl) in livers of american (anguilla rostrata) and european (anguilla anguilla) yellow eels. |
publishDate |
2015 |
url |
https://espace.inrs.ca/id/eprint/3926/ https://doi.org/10.1016/j.aquatox.2015.01.011 |
long_lat |
ENVELOPE(-115.002,-115.002,58.384,58.384) |
geographic |
Canada Lawrence River |
geographic_facet |
Canada Lawrence River |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
Rosabal, Maikel, Pierron, Fabien, Couture, Patrice orcid:0000-0002-1944-5136 , Baudrimont, Magalie, Hare, Landis et Campbell, Peter G. C. (2015). Subcellular partitioning of non-essential trace metals (Ag, As, Cd, Ni, Pb, Tl) in livers of American (Anguilla rostrata) and European (Anguilla anguilla) yellow eels. Aquatic Toxicology , vol. 160 . p. 128-141. DOI:10.1016/j.aquatox.2015.01.011 <https://doi.org/10.1016/j.aquatox.2015.01.011>. doi:10.1016/j.aquatox.2015.01.011 |
op_doi |
https://doi.org/10.1016/j.aquatox.2015.01.011 |
container_title |
Aquatic Toxicology |
container_volume |
160 |
container_start_page |
128 |
op_container_end_page |
141 |
_version_ |
1766396433180655616 |