Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic.
Permafrost thawing in lowland Arctic tundra results in a polygonal patterned landscape and the formation of numerous small ponds. These ponds emit biologically mediated carbon dioxide (CO₂) and methane (CH₄). Their greenhouse gas (GHG) emissions are variable, for reasons that are not well understood...
Published in: | Polar Biology |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2014
|
Subjects: | |
Online Access: | https://espace.inrs.ca/id/eprint/3643/ https://doi.org/10.1007/s00300-014-1555-1 |
id |
ftinrsquebec:oai:espace.inrs.ca:3643 |
---|---|
record_format |
openpolar |
spelling |
ftinrsquebec:oai:espace.inrs.ca:3643 2023-05-15T14:25:32+02:00 Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. Negandhi, Karita Laurion, Isabelle Lovejoy, Connie 2014 https://espace.inrs.ca/id/eprint/3643/ https://doi.org/10.1007/s00300-014-1555-1 unknown Negandhi, Karita, Laurion, Isabelle et Lovejoy, Connie (2014). Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. Polar Biology , vol. 37 , nº 11. p. 1669-1683. DOI:10.1007/s00300-014-1555-1 <https://doi.org/10.1007/s00300-014-1555-1>. doi:10.1007/s00300-014-1555-1 methanotrophic communities carbon thermokarst ponds permafrost methane carbon dioxide pyrosequencing Article Évalué par les pairs 2014 ftinrsquebec https://doi.org/10.1007/s00300-014-1555-1 2023-02-10T11:42:35Z Permafrost thawing in lowland Arctic tundra results in a polygonal patterned landscape and the formation of numerous small ponds. These ponds emit biologically mediated carbon dioxide (CO₂) and methane (CH₄). Their greenhouse gas (GHG) emissions are variable, for reasons that are not well understood. Emissions are related to a balance between GHG producers and consumers, as well as to physical properties of the water column controlling gas exchange rates with the atmosphere. Here, we investigated the bacterial diversity of polygonal and runnel ponds, two geomorphologically distinct pond types commonly found in continuous permafrost regions. Using a combination of 16S rRNA Sanger sequencing and high-throughput amplicon sequencing, we found that bacterial communities in overlying waters were clearly dominated by carbon degraders and were similar in both pond types, despite their variable physical and chemical properties. However, surface sediment communities in the two pond types were significantly different. Polygonal pond sediment was colonized by carbon degraders (46–29 %), cyanobacteria (20–27 %) that take up CO₂ and produce oxygen, and methanotrophs (11–20 %) that consume CH4 and require oxygen. In contrast, cyanobacteria were effectively absent from the surface sediment of runnel ponds, which in addition to carbon degraders (65–81 %), were colonized by purple non-sulfur bacteria (5–21 %), and by fewer methanotrophs (1–5 %). The link between the methanotrophic community and the type of ponds could potentially be used to improve upscale estimates of GHG emissions based on landscape morphology in such remote regions. Article in Journal/Newspaper Arctic Arctic permafrost Polar Biology Thermokarst Tundra Institut national de la recherche scientifique, Québec: Espace INRS Arctic Polar Biology 37 11 1669 1683 |
institution |
Open Polar |
collection |
Institut national de la recherche scientifique, Québec: Espace INRS |
op_collection_id |
ftinrsquebec |
language |
unknown |
topic |
methanotrophic communities carbon thermokarst ponds permafrost methane carbon dioxide pyrosequencing |
spellingShingle |
methanotrophic communities carbon thermokarst ponds permafrost methane carbon dioxide pyrosequencing Negandhi, Karita Laurion, Isabelle Lovejoy, Connie Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. |
topic_facet |
methanotrophic communities carbon thermokarst ponds permafrost methane carbon dioxide pyrosequencing |
description |
Permafrost thawing in lowland Arctic tundra results in a polygonal patterned landscape and the formation of numerous small ponds. These ponds emit biologically mediated carbon dioxide (CO₂) and methane (CH₄). Their greenhouse gas (GHG) emissions are variable, for reasons that are not well understood. Emissions are related to a balance between GHG producers and consumers, as well as to physical properties of the water column controlling gas exchange rates with the atmosphere. Here, we investigated the bacterial diversity of polygonal and runnel ponds, two geomorphologically distinct pond types commonly found in continuous permafrost regions. Using a combination of 16S rRNA Sanger sequencing and high-throughput amplicon sequencing, we found that bacterial communities in overlying waters were clearly dominated by carbon degraders and were similar in both pond types, despite their variable physical and chemical properties. However, surface sediment communities in the two pond types were significantly different. Polygonal pond sediment was colonized by carbon degraders (46–29 %), cyanobacteria (20–27 %) that take up CO₂ and produce oxygen, and methanotrophs (11–20 %) that consume CH4 and require oxygen. In contrast, cyanobacteria were effectively absent from the surface sediment of runnel ponds, which in addition to carbon degraders (65–81 %), were colonized by purple non-sulfur bacteria (5–21 %), and by fewer methanotrophs (1–5 %). The link between the methanotrophic community and the type of ponds could potentially be used to improve upscale estimates of GHG emissions based on landscape morphology in such remote regions. |
format |
Article in Journal/Newspaper |
author |
Negandhi, Karita Laurion, Isabelle Lovejoy, Connie |
author_facet |
Negandhi, Karita Laurion, Isabelle Lovejoy, Connie |
author_sort |
Negandhi, Karita |
title |
Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. |
title_short |
Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. |
title_full |
Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. |
title_fullStr |
Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. |
title_full_unstemmed |
Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. |
title_sort |
bacterial communities and greenhouse gas emissions of shallow ponds in the high arctic. |
publishDate |
2014 |
url |
https://espace.inrs.ca/id/eprint/3643/ https://doi.org/10.1007/s00300-014-1555-1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic permafrost Polar Biology Thermokarst Tundra |
genre_facet |
Arctic Arctic permafrost Polar Biology Thermokarst Tundra |
op_relation |
Negandhi, Karita, Laurion, Isabelle et Lovejoy, Connie (2014). Bacterial communities and greenhouse gas emissions of shallow ponds in the High Arctic. Polar Biology , vol. 37 , nº 11. p. 1669-1683. DOI:10.1007/s00300-014-1555-1 <https://doi.org/10.1007/s00300-014-1555-1>. doi:10.1007/s00300-014-1555-1 |
op_doi |
https://doi.org/10.1007/s00300-014-1555-1 |
container_title |
Polar Biology |
container_volume |
37 |
container_issue |
11 |
container_start_page |
1669 |
op_container_end_page |
1683 |
_version_ |
1766297982187077632 |