Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep
Four ruminal cannulated Texel wethers were used to study the long-term effects of an acidotic diet on ruminal parameters and blood acid-base status. The short-term events around feeding and the recovery of the animals were followed after this nutritional disturbance. Sheep were limit-fed consecutive...
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ftinraparis:oai:prodinra.inra.fr:73625 2023-05-15T15:53:04+02:00 Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep Brossard, Ludovic Martin, Cécile Michalet-Doreau, Brigitte 2003 application/pdf http://prodinra.inra.fr/ft/2A0B8A1C-8C9A-4915-BB1B-406CE646CFBF http://prodinra.inra.fr/record/73625 https://doi.org/10.1051/animres:2003036 eng eng http://creativecommons.org/licenses/by-nd-nc/1.0/ CC-BY-ND-NC Animal Research 6 (52), 513-530. (2003) acidosis;rumen fermentation; protozoa;blood;acid-base status;équilibre acido-basique;sheep;ruminant ovin ruminant acidose fermentation ruminale régime alimentaire nutrition sang ARTICLE 2003 ftinraparis https://doi.org/10.1051/animres:2003036 2017-05-16T22:24:24Z Four ruminal cannulated Texel wethers were used to study the long-term effects of an acidotic diet on ruminal parameters and blood acid-base status. The short-term events around feeding and the recovery of the animals were followed after this nutritional disturbance. Sheep were limit-fed consecutively a control diet H (100% hay) (one week), an acidotic diet W (60% wheat + 40% hay) (2 weeks), and again the control diet (2 weeks). Mean, minimum and maximum ruminal pH were lower, and the time and area under pH 6.0 were higher (P < 0.001) with the W diet than with the H diet. These pH parameters indicate a latent acidosis defined here as a subacute and maintained acidosis. Before feeding, the drop in ruminal pH with the W diet was correlated with an increase in the VFA buffering capacity (BC) (R 2 = 0.70) and with a decrease in the BC of both carbonic acid functions (R 2 = 0.52 for H 2 CO 3 and 0.55 for HCO 3 –). After feeding, the acidotic diet effect on ruminal pH was not explained by variations in the BC of either of these chemical species. Ruminal lactate concentration was higher with the W diet compared to the H diet (P < 0.001) but remained low (< 2 mmol/L). Total VFA concentration (P < 0.001), acetate (P < 0.001) and propionate (P < 0.01) proportions in the rumen decreased with the W diet, while the butyrate proportion increased (P < 0.001). The number of Entodiniomorphs increased with the W diet (P < 0.001). Most parameters showed no significant variation between the 2 weeks with the W diet (P > 0.05). All ruminal parameters, except for ammonia, recovered to initial levels during the H diet redistribution (P < 0.05) while blood parameters decreased (pH, P < 0.05; bicarbonates (HCO 3 –), total CO 2 content (TCO 2), base excess in whole blood (Beb) and in extra cellular fluid (Beecf), P < 0.01). This decrease, initiated during the distribution of the acidotic diet, suggests a mobilization of body alkaline reserves and a longer recovery time in blood than in the rumen. We observed a non-lactic but butyric latent acidosis, linked to Entodiniomorph proliferation, suggesting an intermediate stage before the onset of acute lactic acidosis. Article in Journal/Newspaper Carbonic acid Institut National de la Recherche Agronomique: ProdINRA |
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Open Polar |
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Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
acidosis;rumen fermentation; protozoa;blood;acid-base status;équilibre acido-basique;sheep;ruminant ovin ruminant acidose fermentation ruminale régime alimentaire nutrition sang |
spellingShingle |
acidosis;rumen fermentation; protozoa;blood;acid-base status;équilibre acido-basique;sheep;ruminant ovin ruminant acidose fermentation ruminale régime alimentaire nutrition sang Brossard, Ludovic Martin, Cécile Michalet-Doreau, Brigitte Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
topic_facet |
acidosis;rumen fermentation; protozoa;blood;acid-base status;équilibre acido-basique;sheep;ruminant ovin ruminant acidose fermentation ruminale régime alimentaire nutrition sang |
description |
Four ruminal cannulated Texel wethers were used to study the long-term effects of an acidotic diet on ruminal parameters and blood acid-base status. The short-term events around feeding and the recovery of the animals were followed after this nutritional disturbance. Sheep were limit-fed consecutively a control diet H (100% hay) (one week), an acidotic diet W (60% wheat + 40% hay) (2 weeks), and again the control diet (2 weeks). Mean, minimum and maximum ruminal pH were lower, and the time and area under pH 6.0 were higher (P < 0.001) with the W diet than with the H diet. These pH parameters indicate a latent acidosis defined here as a subacute and maintained acidosis. Before feeding, the drop in ruminal pH with the W diet was correlated with an increase in the VFA buffering capacity (BC) (R 2 = 0.70) and with a decrease in the BC of both carbonic acid functions (R 2 = 0.52 for H 2 CO 3 and 0.55 for HCO 3 –). After feeding, the acidotic diet effect on ruminal pH was not explained by variations in the BC of either of these chemical species. Ruminal lactate concentration was higher with the W diet compared to the H diet (P < 0.001) but remained low (< 2 mmol/L). Total VFA concentration (P < 0.001), acetate (P < 0.001) and propionate (P < 0.01) proportions in the rumen decreased with the W diet, while the butyrate proportion increased (P < 0.001). The number of Entodiniomorphs increased with the W diet (P < 0.001). Most parameters showed no significant variation between the 2 weeks with the W diet (P > 0.05). All ruminal parameters, except for ammonia, recovered to initial levels during the H diet redistribution (P < 0.05) while blood parameters decreased (pH, P < 0.05; bicarbonates (HCO 3 –), total CO 2 content (TCO 2), base excess in whole blood (Beb) and in extra cellular fluid (Beecf), P < 0.01). This decrease, initiated during the distribution of the acidotic diet, suggests a mobilization of body alkaline reserves and a longer recovery time in blood than in the rumen. We observed a non-lactic but butyric latent acidosis, linked to Entodiniomorph proliferation, suggesting an intermediate stage before the onset of acute lactic acidosis. |
format |
Article in Journal/Newspaper |
author |
Brossard, Ludovic Martin, Cécile Michalet-Doreau, Brigitte |
author_facet |
Brossard, Ludovic Martin, Cécile Michalet-Doreau, Brigitte |
author_sort |
Brossard, Ludovic |
title |
Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
title_short |
Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
title_full |
Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
title_fullStr |
Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
title_full_unstemmed |
Ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
title_sort |
ruminal fermentative parameters and blood acido-basic balance changes during the onset and recovery of induced latent acidosis in sheep |
publishDate |
2003 |
url |
http://prodinra.inra.fr/ft/2A0B8A1C-8C9A-4915-BB1B-406CE646CFBF http://prodinra.inra.fr/record/73625 https://doi.org/10.1051/animres:2003036 |
genre |
Carbonic acid |
genre_facet |
Carbonic acid |
op_source |
Animal Research 6 (52), 513-530. (2003) |
op_rights |
http://creativecommons.org/licenses/by-nd-nc/1.0/ |
op_rightsnorm |
CC-BY-ND-NC |
op_doi |
https://doi.org/10.1051/animres:2003036 |
_version_ |
1766388134059180032 |