Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation
Acquisition of sperm fertilizing ability is due, in part, to the reorganization of plasma membrane proteins that occurs during epididymal sperm transit. Using polyclonal antibodies against angiotensin I-converting enzyme (ACE), we showed that this enzyme is immunolocalized mainly on the middle piece...
Published in: | Biology of Reproduction |
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Online Access: | http://prodinra.inra.fr/ft/41269CC7-69AA-4AE3-9EE8-E7162DC4A3D1 http://prodinra.inra.fr/record/68685 https://doi.org/10.1095/biolreprod.102.006684 |
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ftinraparis:oai:prodinra.inra.fr:68685 2023-05-15T18:05:27+02:00 Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation Metayer-Coustard, Sonia Dacheux, F. Dacheux, J.L. Gatti, Jean-Luc 2002 application/pdf http://prodinra.inra.fr/ft/41269CC7-69AA-4AE3-9EE8-E7162DC4A3D1 http://prodinra.inra.fr/record/68685 https://doi.org/10.1095/biolreprod.102.006684 eng eng https://creativecommons.org/licenses/by-sa/3.0/ CC-BY-SA Biology of Reproduction 6 (67), 1763-1767. (2002) Biologie de la reproduction Reproductive Biology RAT rattus rattus spermatozoide mâle épididyme maturation pouvoir fecondant reproduction protéine enzyme angiotensine ARTICLE 2002 ftinraparis https://doi.org/10.1095/biolreprod.102.006684 2019-04-09T22:25:08Z Acquisition of sperm fertilizing ability is due, in part, to the reorganization of plasma membrane proteins that occurs during epididymal sperm transit. Using polyclonal antibodies against angiotensin I-converting enzyme (ACE), we showed that this enzyme is immunolocalized mainly on the middle piece of rat and mouse testicular sperm and with less intensity along the initial part of the principal piece of the flagellum. In both species, only some sperm from the caput epididymis were still reactive, whereas no labeling was observed on cauda epididymal sperm. The 105- to 110-kDa germinal ACE was absent from the rat testicular fluid but appeared in the fluid of the anterior epididymis. Thereafter, its molecular weight shifted to 94 kDa in the corpus epididymal fluid and remained at this weight in the caudal region. The 105- to 110-kDa immunoreactive protein was present in testicular rat sperm extract but was completely absent from epididymal sperm extracts. Western blot analysis of testicular and epididymal tissue extracts from the rat and mouse also confirmed that the germinal enzyme was absent from the epididymal sperm cell. Our results demonstrated that the rodent germinal ACE is released from the testicular sperm membrane when sperm enter the epididymis, a process similar to that observed in domestic mammals. This result is discussed in view of the suggested role for this enzyme in sperm fertility. Article in Journal/Newspaper Rattus rattus Institut National de la Recherche Agronomique: ProdINRA Biology of Reproduction 67 6 1763 1767 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
Biologie de la reproduction Reproductive Biology RAT rattus rattus spermatozoide mâle épididyme maturation pouvoir fecondant reproduction protéine enzyme angiotensine |
spellingShingle |
Biologie de la reproduction Reproductive Biology RAT rattus rattus spermatozoide mâle épididyme maturation pouvoir fecondant reproduction protéine enzyme angiotensine Metayer-Coustard, Sonia Dacheux, F. Dacheux, J.L. Gatti, Jean-Luc Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
topic_facet |
Biologie de la reproduction Reproductive Biology RAT rattus rattus spermatozoide mâle épididyme maturation pouvoir fecondant reproduction protéine enzyme angiotensine |
description |
Acquisition of sperm fertilizing ability is due, in part, to the reorganization of plasma membrane proteins that occurs during epididymal sperm transit. Using polyclonal antibodies against angiotensin I-converting enzyme (ACE), we showed that this enzyme is immunolocalized mainly on the middle piece of rat and mouse testicular sperm and with less intensity along the initial part of the principal piece of the flagellum. In both species, only some sperm from the caput epididymis were still reactive, whereas no labeling was observed on cauda epididymal sperm. The 105- to 110-kDa germinal ACE was absent from the rat testicular fluid but appeared in the fluid of the anterior epididymis. Thereafter, its molecular weight shifted to 94 kDa in the corpus epididymal fluid and remained at this weight in the caudal region. The 105- to 110-kDa immunoreactive protein was present in testicular rat sperm extract but was completely absent from epididymal sperm extracts. Western blot analysis of testicular and epididymal tissue extracts from the rat and mouse also confirmed that the germinal enzyme was absent from the epididymal sperm cell. Our results demonstrated that the rodent germinal ACE is released from the testicular sperm membrane when sperm enter the epididymis, a process similar to that observed in domestic mammals. This result is discussed in view of the suggested role for this enzyme in sperm fertility. |
format |
Article in Journal/Newspaper |
author |
Metayer-Coustard, Sonia Dacheux, F. Dacheux, J.L. Gatti, Jean-Luc |
author_facet |
Metayer-Coustard, Sonia Dacheux, F. Dacheux, J.L. Gatti, Jean-Luc |
author_sort |
Metayer-Coustard, Sonia |
title |
Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
title_short |
Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
title_full |
Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
title_fullStr |
Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
title_full_unstemmed |
Germinal angiotensin I-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
title_sort |
germinal angiotensin i-converting enzyme is totally shed from the rodent sperm membrane during epididymal maturation |
publishDate |
2002 |
url |
http://prodinra.inra.fr/ft/41269CC7-69AA-4AE3-9EE8-E7162DC4A3D1 http://prodinra.inra.fr/record/68685 https://doi.org/10.1095/biolreprod.102.006684 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Biology of Reproduction 6 (67), 1763-1767. (2002) |
op_rights |
https://creativecommons.org/licenses/by-sa/3.0/ |
op_rightsnorm |
CC-BY-SA |
op_doi |
https://doi.org/10.1095/biolreprod.102.006684 |
container_title |
Biology of Reproduction |
container_volume |
67 |
container_issue |
6 |
container_start_page |
1763 |
op_container_end_page |
1767 |
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1766176922481459200 |