Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar
Fish are the second most widely utilized vertebrate group used for scientific procedures in the United Kingdom, but the development and application of 3Rs (the principles of replacement, reduction, and refinement) in aquaculture disease research lags behind methodologies in place for mammalian studi...
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Online Access: | http://prodinra.inra.fr/ft/17B30687-52D7-431B-8331-D842E61175C7 http://prodinra.inra.fr/record/468704 https://doi.org/10.1111/jfb.13803 |
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ftinraparis:oai:prodinra.inra.fr:468704 2023-05-15T15:31:04+02:00 Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar Cameron, Gary A. Fordyce, Mark Noguera, Patricia Wang, Tiehui Collins, Catherine Secombes, Christopher J. Collet, Bertrand Chance, Rachel J. 2018 application/pdf http://prodinra.inra.fr/ft/17B30687-52D7-431B-8331-D842E61175C7 http://prodinra.inra.fr/record/468704 https://doi.org/10.1111/jfb.13803 eng eng https://creativecommons.org/licenses/by/3.0/ CC-BY Journal of Fish Biology 6 (93), 1069-1081. (2018) anaesthesia;metomidate;non-lethal stress;atlantic salmon ARTICLE 2018 ftinraparis https://doi.org/10.1111/jfb.13803 2019-05-14T22:25:33Z Fish are the second most widely utilized vertebrate group used for scientific procedures in the United Kingdom, but the development and application of 3Rs (the principles of replacement, reduction, and refinement) in aquaculture disease research lags behind methodologies in place for mammalian studies. With a need for individual monitoring and non-lethal sampling, the effect of repeat anaesthesia on experimental fish needs to be better understood. This study analyses the effect of repeat anaesthesia with MS-222, metomidate and AQUI-S upon the gill and general health of post-smolt Atlantic salmon Salmo salar. A single, lethal dose of anaesthetic was compared with seven anaesthetizing time points over 28 days, terminating in a lethal dose. No anaesthetic showed significant differences in accumulation in the muscle tissue, or changes in plasma glucose after repeated or single dosing. Fish repeatedly anaesthetized with MS-222 or AQUI-S exhibited upregulation of osmoregulatory genes in the gill and AQUI-S-treated individuals showed, histologically, epithelial lifting from the lamellae capillary irrespective of whether they had a single or repeated dose history. No significant changes were seen in inflammatory or stress genes in the head kidney of fish repeatedly anaesthetized with AQUI-S or metomidate, however MS-222 treatment resulted in upregulation of tnf alpha 3. Repeated anaesthesia with MS-222 and metomidate gave a significant decrease and increase in peripheral blood neutrophils, respectively. This study concludes that no increase in cumulative stress or inflammation is induced by the repeated anaesthetization of S. salar with any of the tested anaesthetics, however gill osmotic regulation and blood parameters may be affected. Article in Journal/Newspaper Atlantic salmon Salmo salar Institut National de la Recherche Agronomique: ProdINRA Journal of Fish Biology 93 6 1069 1081 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
anaesthesia;metomidate;non-lethal stress;atlantic salmon |
spellingShingle |
anaesthesia;metomidate;non-lethal stress;atlantic salmon Cameron, Gary A. Fordyce, Mark Noguera, Patricia Wang, Tiehui Collins, Catherine Secombes, Christopher J. Collet, Bertrand Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar |
topic_facet |
anaesthesia;metomidate;non-lethal stress;atlantic salmon |
description |
Fish are the second most widely utilized vertebrate group used for scientific procedures in the United Kingdom, but the development and application of 3Rs (the principles of replacement, reduction, and refinement) in aquaculture disease research lags behind methodologies in place for mammalian studies. With a need for individual monitoring and non-lethal sampling, the effect of repeat anaesthesia on experimental fish needs to be better understood. This study analyses the effect of repeat anaesthesia with MS-222, metomidate and AQUI-S upon the gill and general health of post-smolt Atlantic salmon Salmo salar. A single, lethal dose of anaesthetic was compared with seven anaesthetizing time points over 28 days, terminating in a lethal dose. No anaesthetic showed significant differences in accumulation in the muscle tissue, or changes in plasma glucose after repeated or single dosing. Fish repeatedly anaesthetized with MS-222 or AQUI-S exhibited upregulation of osmoregulatory genes in the gill and AQUI-S-treated individuals showed, histologically, epithelial lifting from the lamellae capillary irrespective of whether they had a single or repeated dose history. No significant changes were seen in inflammatory or stress genes in the head kidney of fish repeatedly anaesthetized with AQUI-S or metomidate, however MS-222 treatment resulted in upregulation of tnf alpha 3. Repeated anaesthesia with MS-222 and metomidate gave a significant decrease and increase in peripheral blood neutrophils, respectively. This study concludes that no increase in cumulative stress or inflammation is induced by the repeated anaesthetization of S. salar with any of the tested anaesthetics, however gill osmotic regulation and blood parameters may be affected. |
author2 |
Chance, Rachel J. |
format |
Article in Journal/Newspaper |
author |
Cameron, Gary A. Fordyce, Mark Noguera, Patricia Wang, Tiehui Collins, Catherine Secombes, Christopher J. Collet, Bertrand |
author_facet |
Cameron, Gary A. Fordyce, Mark Noguera, Patricia Wang, Tiehui Collins, Catherine Secombes, Christopher J. Collet, Bertrand |
author_sort |
Cameron, Gary A. |
title |
Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar |
title_short |
Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar |
title_full |
Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar |
title_fullStr |
Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar |
title_full_unstemmed |
Effects of repeated anaesthesia on gill and general health of Atlantic salmon, salmo salar |
title_sort |
effects of repeated anaesthesia on gill and general health of atlantic salmon, salmo salar |
publishDate |
2018 |
url |
http://prodinra.inra.fr/ft/17B30687-52D7-431B-8331-D842E61175C7 http://prodinra.inra.fr/record/468704 https://doi.org/10.1111/jfb.13803 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Fish Biology 6 (93), 1069-1081. (2018) |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/jfb.13803 |
container_title |
Journal of Fish Biology |
container_volume |
93 |
container_issue |
6 |
container_start_page |
1069 |
op_container_end_page |
1081 |
_version_ |
1766361556758560768 |