Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli
Myostatin and hypoxia signalling pathways are able to induce skeletal muscle atrophy, but whether a relationship between these two pathways exists is currently unknown. Here, we tested the hypothesis that a potential mechanism for hypoxia effect on skeletal muscle may be through regulation of myosta...
Published in: | Molecular and Cellular Endocrinology |
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ftinraparis:oai:prodinra.inra.fr:45915 2023-05-15T18:05:20+02:00 Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli Hayot, Maurice Rodriguez, Julie Vernus, Barbara Carnac, Gilles Jean, Elise Allen, David Goret, Lucie Obert, Philippe Candau, Robin Bonnieu, Anne 2011 application/pdf http://prodinra.inra.fr/ft/131994A2-EDF6-4E94-B41C-DB874D2D7DC8 http://prodinra.inra.fr/record/45915 https://doi.org/10.1016/j.mce.2010.09.008 eng eng http://creativecommons.org/licenses/by-nd-nc/1.0/ CC-BY-ND-NC Molecular and Cellular Endocrinology 1-2 (332), 38–47. (2011) HYPOXIC STIMULUS;MYOSTATIN;SKELETAL MUSCLE ATROPHY;COPD;MYOTUBES ;RAT;MALADIE PULMONAIRE OBSTRUCTIVE CHRONIQUE rattus rattus anticorps maladie respiratoire maladie génétique atrophie musculaire hypoxie myostatine ARTICLE 2011 ftinraparis https://doi.org/10.1016/j.mce.2010.09.008 2015-11-10T23:26:50Z Myostatin and hypoxia signalling pathways are able to induce skeletal muscle atrophy, but whether a relationship between these two pathways exists is currently unknown. Here, we tested the hypothesis that a potential mechanism for hypoxia effect on skeletal muscle may be through regulation of myostatin. We reported an induction of myostatin expression in muscles of rats exposed to chronic hypoxia. Interestingly, we also demonstrated increased skeletal muscle myostatin protein expression in skeletal muscle of hypoxemic patients with severe chronic obstructive pulmonary disease (COPD). Parallel studies in human skeletal muscle cell cultures showed that induction of myostatin expression in myotubes treated with hypoxia-mimicking agent such as cobalt chloride (CoCl2) is associated with myotube atrophy. Furthermore, we demonstrated that inhibition of myostatin by means of genetic deletion of myostatin or treatment with blocking antimyostatin antibodies inhibits the CoCl2-induced atrophy in muscle cells. Finally, addition of recombinant myostatin restored the CoCl2-induced atrophy in myostatin deficient myotubes. These results strongly suggest that myostatin can play an essential role in the adaptation of skeletal muscle to hypoxic environment Article in Journal/Newspaper Rattus rattus Institut National de la Recherche Agronomique: ProdINRA Molecular and Cellular Endocrinology 332 1-2 38 47 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
HYPOXIC STIMULUS;MYOSTATIN;SKELETAL MUSCLE ATROPHY;COPD;MYOTUBES ;RAT;MALADIE PULMONAIRE OBSTRUCTIVE CHRONIQUE rattus rattus anticorps maladie respiratoire maladie génétique atrophie musculaire hypoxie myostatine |
spellingShingle |
HYPOXIC STIMULUS;MYOSTATIN;SKELETAL MUSCLE ATROPHY;COPD;MYOTUBES ;RAT;MALADIE PULMONAIRE OBSTRUCTIVE CHRONIQUE rattus rattus anticorps maladie respiratoire maladie génétique atrophie musculaire hypoxie myostatine Hayot, Maurice Rodriguez, Julie Vernus, Barbara Carnac, Gilles Jean, Elise Allen, David Goret, Lucie Obert, Philippe Candau, Robin Bonnieu, Anne Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
topic_facet |
HYPOXIC STIMULUS;MYOSTATIN;SKELETAL MUSCLE ATROPHY;COPD;MYOTUBES ;RAT;MALADIE PULMONAIRE OBSTRUCTIVE CHRONIQUE rattus rattus anticorps maladie respiratoire maladie génétique atrophie musculaire hypoxie myostatine |
description |
Myostatin and hypoxia signalling pathways are able to induce skeletal muscle atrophy, but whether a relationship between these two pathways exists is currently unknown. Here, we tested the hypothesis that a potential mechanism for hypoxia effect on skeletal muscle may be through regulation of myostatin. We reported an induction of myostatin expression in muscles of rats exposed to chronic hypoxia. Interestingly, we also demonstrated increased skeletal muscle myostatin protein expression in skeletal muscle of hypoxemic patients with severe chronic obstructive pulmonary disease (COPD). Parallel studies in human skeletal muscle cell cultures showed that induction of myostatin expression in myotubes treated with hypoxia-mimicking agent such as cobalt chloride (CoCl2) is associated with myotube atrophy. Furthermore, we demonstrated that inhibition of myostatin by means of genetic deletion of myostatin or treatment with blocking antimyostatin antibodies inhibits the CoCl2-induced atrophy in muscle cells. Finally, addition of recombinant myostatin restored the CoCl2-induced atrophy in myostatin deficient myotubes. These results strongly suggest that myostatin can play an essential role in the adaptation of skeletal muscle to hypoxic environment |
format |
Article in Journal/Newspaper |
author |
Hayot, Maurice Rodriguez, Julie Vernus, Barbara Carnac, Gilles Jean, Elise Allen, David Goret, Lucie Obert, Philippe Candau, Robin Bonnieu, Anne |
author_facet |
Hayot, Maurice Rodriguez, Julie Vernus, Barbara Carnac, Gilles Jean, Elise Allen, David Goret, Lucie Obert, Philippe Candau, Robin Bonnieu, Anne |
author_sort |
Hayot, Maurice |
title |
Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
title_short |
Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
title_full |
Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
title_fullStr |
Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
title_full_unstemmed |
Myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
title_sort |
myostatin up-regulation is associated with the skeletal muscle response to hypoxic stimuli |
publishDate |
2011 |
url |
http://prodinra.inra.fr/ft/131994A2-EDF6-4E94-B41C-DB874D2D7DC8 http://prodinra.inra.fr/record/45915 https://doi.org/10.1016/j.mce.2010.09.008 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Molecular and Cellular Endocrinology 1-2 (332), 38–47. (2011) |
op_rights |
http://creativecommons.org/licenses/by-nd-nc/1.0/ |
op_rightsnorm |
CC-BY-ND-NC |
op_doi |
https://doi.org/10.1016/j.mce.2010.09.008 |
container_title |
Molecular and Cellular Endocrinology |
container_volume |
332 |
container_issue |
1-2 |
container_start_page |
38 |
op_container_end_page |
47 |
_version_ |
1766176801292288000 |