ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport
Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has...
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ftinraparis:oai:prodinra.inra.fr:41793 2023-05-15T16:06:46+02:00 ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloise North, Helen Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Marie-Hélène 2011 application/pdf http://prodinra.inra.fr/ft/4BBDF148-1537-412C-8B12-130CECF5AFC4 http://prodinra.inra.fr/record/41793 https://doi.org/10.1371/journal.pone.0016645 eng eng http://creativecommons.org/licenses/by-nd-nc/1.0/ CC-BY-ND-NC Plos One 2 (6), e16645. (2011) WATER;ABSCISIC ACID;WATER STRESS;MEMBRANE TRANSPORT;ROOT;STOMATA;AQUAPORIN;XYLEM;BIOLOGIE MOLECULAIRE;BIOLOGIE DU DEVELOPPEMENT arabidopsis racine xylème physiologie végétale stress abiotique stress hydrique ARTICLE 2011 ftinraparis https://doi.org/10.1371/journal.pone.0016645 2015-10-30T07:41:45Z Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components. Article in Journal/Newspaper eskimo* Institut National de la Recherche Agronomique: ProdINRA PLoS ONE 6 2 e16645 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
WATER;ABSCISIC ACID;WATER STRESS;MEMBRANE TRANSPORT;ROOT;STOMATA;AQUAPORIN;XYLEM;BIOLOGIE MOLECULAIRE;BIOLOGIE DU DEVELOPPEMENT arabidopsis racine xylème physiologie végétale stress abiotique stress hydrique |
spellingShingle |
WATER;ABSCISIC ACID;WATER STRESS;MEMBRANE TRANSPORT;ROOT;STOMATA;AQUAPORIN;XYLEM;BIOLOGIE MOLECULAIRE;BIOLOGIE DU DEVELOPPEMENT arabidopsis racine xylème physiologie végétale stress abiotique stress hydrique Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloise North, Helen Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Marie-Hélène ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
topic_facet |
WATER;ABSCISIC ACID;WATER STRESS;MEMBRANE TRANSPORT;ROOT;STOMATA;AQUAPORIN;XYLEM;BIOLOGIE MOLECULAIRE;BIOLOGIE DU DEVELOPPEMENT arabidopsis racine xylème physiologie végétale stress abiotique stress hydrique |
description |
Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components. |
format |
Article in Journal/Newspaper |
author |
Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloise North, Helen Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Marie-Hélène |
author_facet |
Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloise North, Helen Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Marie-Hélène |
author_sort |
Lefebvre, Valérie |
title |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_short |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_full |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_fullStr |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_full_unstemmed |
ESKIMO1 Disruption in Arabidopsis Alters Vascular Tissue and Impairs Water Transport |
title_sort |
eskimo1 disruption in arabidopsis alters vascular tissue and impairs water transport |
publishDate |
2011 |
url |
http://prodinra.inra.fr/ft/4BBDF148-1537-412C-8B12-130CECF5AFC4 http://prodinra.inra.fr/record/41793 https://doi.org/10.1371/journal.pone.0016645 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
Plos One 2 (6), e16645. (2011) |
op_rights |
http://creativecommons.org/licenses/by-nd-nc/1.0/ |
op_rightsnorm |
CC-BY-ND-NC |
op_doi |
https://doi.org/10.1371/journal.pone.0016645 |
container_title |
PLoS ONE |
container_volume |
6 |
container_issue |
2 |
container_start_page |
e16645 |
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1766402798176436224 |