The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway
Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, incl...
Published in: | Aquatic Toxicology |
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Online Access: | http://prodinra.inra.fr/ft/D2149F7A-6488-408C-921E-625FF3A17EEF http://prodinra.inra.fr/ft/AC28EA72-C884-4246-AD90-4A980BA5EEEE http://prodinra.inra.fr/record/398796 https://doi.org/10.1016/j.aquatox.2017.07.001 |
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ftinraparis:oai:prodinra.inra.fr:398796 2023-05-15T15:57:58+02:00 The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine Rolland, Jean Luc 2017 application/pdf http://prodinra.inra.fr/ft/D2149F7A-6488-408C-921E-625FF3A17EEF http://prodinra.inra.fr/ft/AC28EA72-C884-4246-AD90-4A980BA5EEEE http://prodinra.inra.fr/record/398796 https://doi.org/10.1016/j.aquatox.2017.07.001 eng eng https://creativecommons.org/licenses/by-sa/3.0/ https://creativecommons.org/licenses/by-nc-nd/3.0/ CC-BY-SA CC-BY-NC-ND Aquatic Toxicology (190), 133-141. (2017) Shellfish;Toxin;Harmful algae;Phytoplankton crassostrea gigas hémocyte réaction hémocytaire ARTICLE 2017 ftinraparis https://doi.org/10.1016/j.aquatox.2017.07.001 2020-01-21T23:25:44Z Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which is present in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalve molluscs to microbial infection. Article in Journal/Newspaper Crassostrea gigas Institut National de la Recherche Agronomique: ProdINRA Aquatic Toxicology 190 133 141 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
Shellfish;Toxin;Harmful algae;Phytoplankton crassostrea gigas hémocyte réaction hémocytaire |
spellingShingle |
Shellfish;Toxin;Harmful algae;Phytoplankton crassostrea gigas hémocyte réaction hémocytaire Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
topic_facet |
Shellfish;Toxin;Harmful algae;Phytoplankton crassostrea gigas hémocyte réaction hémocytaire |
description |
Exposure of the toxin-producing dinoflagellate Alexandrium catenella (A. catenella) was previously demonstrated to cause apoptosis of hemocytes in the oyster species Crassostrea gigas. In this work, a coumarin-labeled saxitoxin appeared to spread throughout the cytoplasm of the hemocytes. PSTs, including saxitoxin, were also shown to be directly responsible for inducing apoptosis in hemocytes, a process dependent on caspase activation and independent of reactive oxygen species (ROS) production. A series of in vitro labeling and microscopy experiments revealed that STX and analogs there of induced nuclear condensation, phosphatidylserine exposure, membrane permeability, and DNA fragmentation of hemocytes. Unlike in vertebrates, gonyautoxin-5 (GTX5), which is present in high concentrations in A. catenella, was found to be more toxic than saxitoxin (STX) to oyster immune cells. Altogether, results show that PSTs produced by toxic dinoflagellates enter the cytoplasm and induce apoptosis of oyster immune cells through a caspase-dependent pathway. Because of the central role of hemocytes in mollusc immune defense, PST-induced death of hemocytes could negatively affect resistance of bivalve molluscs to microbial infection. |
author2 |
Rolland, Jean Luc |
format |
Article in Journal/Newspaper |
author |
Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine |
author_facet |
Abi-Khalil, Celina Finkelstein, Darren S. Conejero, Geneviève Du Bois, Justin Destoumieux-Garzon, Delphine |
author_sort |
Abi-Khalil, Celina |
title |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_short |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_full |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_fullStr |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_full_unstemmed |
The paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
title_sort |
paralytic shellfish toxin, saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells through a caspase-dependent pathway |
publishDate |
2017 |
url |
http://prodinra.inra.fr/ft/D2149F7A-6488-408C-921E-625FF3A17EEF http://prodinra.inra.fr/ft/AC28EA72-C884-4246-AD90-4A980BA5EEEE http://prodinra.inra.fr/record/398796 https://doi.org/10.1016/j.aquatox.2017.07.001 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Aquatic Toxicology (190), 133-141. (2017) |
op_rights |
https://creativecommons.org/licenses/by-sa/3.0/ https://creativecommons.org/licenses/by-nc-nd/3.0/ |
op_rightsnorm |
CC-BY-SA CC-BY-NC-ND |
op_doi |
https://doi.org/10.1016/j.aquatox.2017.07.001 |
container_title |
Aquatic Toxicology |
container_volume |
190 |
container_start_page |
133 |
op_container_end_page |
141 |
_version_ |
1766393681492836352 |