Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes
Background: In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF regulates the second meiotic division by blocking secondary spermatocytes in metaphase (metaphase II), and thereby lowers round spermatid formation. In vertebrates, mature oocytes are arrested at metaphase II until fe...
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ftinraparis:oai:prodinra.inra.fr:36655 2023-05-15T18:05:30+02:00 Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes Perrard, Marie-Hélène Chassaing, Emeric Montillet, Guillaume Sabido, Odile Durand, Philippe 2009 application/pdf http://prodinra.inra.fr/ft/70C4D870-59BF-46FC-82EB-49DC0199BF7E http://prodinra.inra.fr/record/36655 https://doi.org/10.1371/journal.pone.0007237 eng eng http://creativecommons.org/licenses/by-nd-nc/1.0/ CC-BY-ND-NC Plos One 10 (4), e7237. (2009) rattus rattus cytoplasme spermatocyte cellule de sertoli métabolisme méiose spermatogénèse facteur de croissance ARTICLE 2009 ftinraparis https://doi.org/10.1371/journal.pone.0007237 2015-10-30T07:22:29Z Background: In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF regulates the second meiotic division by blocking secondary spermatocytes in metaphase (metaphase II), and thereby lowers round spermatid formation. In vertebrates, mature oocytes are arrested at metaphase II until fertilization, because of the presence of cytostatic factor (CSF) in their cytoplasm. By analogy, we hypothesized the presence of CSF in male germ cells. Methodology/Principal Findings: We show here, that Mos, Emi2, cyclin E and Cdk2, the four proteins of CSF, and their respective mRNAs, are present in male rat meiotic cells; this was assessed by using Western blotting, immunocytochemistry and reverse transcriptase PCR. We measured the relative cellular levels of Mos, Emi2, Cyclin E and Cdk2 in the meiotic cells by flow cytometry and found that the four proteins increased throughout the first meiotic prophase, reaching their highest levels in middle to late pachytene spermatocytes, then decreased following the meiotic divisions. In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF increased the number of metaphases II, while enhancing Mos and Emi2 levels in middle to late pachytene spermatocytes, pachytene spermatocytes in division and secondary spermatocytes. Conclusion/Significance: Our results suggest that CSF is not restricted to the oocyte. In addition, they reinforce the view that NGF, by enhancing Mos in late spermatocytes, is one of the intra-testicular factors which adjusts the number of round spermatids that can be supported by Sertoli cells. Article in Journal/Newspaper Rattus rattus Institut National de la Recherche Agronomique: ProdINRA PLoS ONE 4 10 e7237 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
rattus rattus cytoplasme spermatocyte cellule de sertoli métabolisme méiose spermatogénèse facteur de croissance |
spellingShingle |
rattus rattus cytoplasme spermatocyte cellule de sertoli métabolisme méiose spermatogénèse facteur de croissance Perrard, Marie-Hélène Chassaing, Emeric Montillet, Guillaume Sabido, Odile Durand, Philippe Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes |
topic_facet |
rattus rattus cytoplasme spermatocyte cellule de sertoli métabolisme méiose spermatogénèse facteur de croissance |
description |
Background: In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF regulates the second meiotic division by blocking secondary spermatocytes in metaphase (metaphase II), and thereby lowers round spermatid formation. In vertebrates, mature oocytes are arrested at metaphase II until fertilization, because of the presence of cytostatic factor (CSF) in their cytoplasm. By analogy, we hypothesized the presence of CSF in male germ cells. Methodology/Principal Findings: We show here, that Mos, Emi2, cyclin E and Cdk2, the four proteins of CSF, and their respective mRNAs, are present in male rat meiotic cells; this was assessed by using Western blotting, immunocytochemistry and reverse transcriptase PCR. We measured the relative cellular levels of Mos, Emi2, Cyclin E and Cdk2 in the meiotic cells by flow cytometry and found that the four proteins increased throughout the first meiotic prophase, reaching their highest levels in middle to late pachytene spermatocytes, then decreased following the meiotic divisions. In co-cultures of pachytene spermatocytes with Sertoli cells, beta-NGF increased the number of metaphases II, while enhancing Mos and Emi2 levels in middle to late pachytene spermatocytes, pachytene spermatocytes in division and secondary spermatocytes. Conclusion/Significance: Our results suggest that CSF is not restricted to the oocyte. In addition, they reinforce the view that NGF, by enhancing Mos in late spermatocytes, is one of the intra-testicular factors which adjusts the number of round spermatids that can be supported by Sertoli cells. |
format |
Article in Journal/Newspaper |
author |
Perrard, Marie-Hélène Chassaing, Emeric Montillet, Guillaume Sabido, Odile Durand, Philippe |
author_facet |
Perrard, Marie-Hélène Chassaing, Emeric Montillet, Guillaume Sabido, Odile Durand, Philippe |
author_sort |
Perrard, Marie-Hélène |
title |
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes |
title_short |
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes |
title_full |
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes |
title_fullStr |
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes |
title_full_unstemmed |
Cytostatic Factor Proteins Are Present in Male Meiotic Cells and beta-Nerve Growth Factor Increases Mos Levels in Rat Late Spermatocytes |
title_sort |
cytostatic factor proteins are present in male meiotic cells and beta-nerve growth factor increases mos levels in rat late spermatocytes |
publishDate |
2009 |
url |
http://prodinra.inra.fr/ft/70C4D870-59BF-46FC-82EB-49DC0199BF7E http://prodinra.inra.fr/record/36655 https://doi.org/10.1371/journal.pone.0007237 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Plos One 10 (4), e7237. (2009) |
op_rights |
http://creativecommons.org/licenses/by-nd-nc/1.0/ |
op_rightsnorm |
CC-BY-ND-NC |
op_doi |
https://doi.org/10.1371/journal.pone.0007237 |
container_title |
PLoS ONE |
container_volume |
4 |
container_issue |
10 |
container_start_page |
e7237 |
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1766176981106294784 |