Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout
The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the di...
Published in: | Journal of Comparative Neurology |
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1994
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ftinraparis:oai:prodinra.inra.fr:110804 2023-05-15T15:29:58+02:00 Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout Bailhache, Arazam, Klungland, Alestrom, Breton, B. Jego 1994 application/pdf http://prodinra.inra.fr/ft/79071C86-17B3-4AF5-8F2D-D667EFF024BD http://prodinra.inra.fr/record/110804 https://doi.org/10.1002/cne.903470310 eng eng http://creativecommons.org/licenses/by-nd-nc/1.0/ CC-BY-ND-NC Journal of Comparative Neurology (347), 444-454. (1994) salmon gonadotropin-releasing hormone;in situ hybridization;immunocytochemistry saumon de l'atlantique truite arc en ciel salmonidae salmo salar oncorhynchus mykiss poisson arn messager système nerveux central acide nucléique neuropeptide gnrh hybridation in situ méthode northern blot reproduction ARTICLE 1994 ftinraparis https://doi.org/10.1002/cne.903470310 2018-01-16T23:24:49Z The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the distribution of sGnRH perikarya led us to investigate this problem in two species, the Atlantic salmon and the rainbow trout, with in situ hybridization of sGnRH messenger, a highly specific molecular tool. By Northern blot analysis, the rainbow trout sGnRH messenger appears to be about 500 bases in length, which is close to those isolated from Atlantic salmon or masu salmon and characterized previously. In situ hybridization with riboprobes generated with Atlantic salmon sGnRH cDNA demonstrated that sGnRH perikarya are restricted to the ventral part of olfactory bulbs, telencephalon, and preoptic area. They are distributed on a nearly continuous line extending from the olfactory bulbs to the preoptic area in both salmonid species studied. Despite the presence of GnRH-like immunoreactivity in the preoptic magnocellular nucleus (NPOm) and in the tegmentum of the midbrain (MT), the sGnRH mRNA is not present in these two structures. Stained cells in NPOm could be target cells for GnRH and immunoreactive neurons in MT are likely to be chicken GnRH-II containing cells. Our study not only gives a precise distribution of the sGnRH system in two salmonids, Atlantic salmon and rainbow trout, but also clarifies the ambiguous data published up to now in rainbow trout Article in Journal/Newspaper Atlantic salmon Salmo salar Institut National de la Recherche Agronomique: ProdINRA Journal of Comparative Neurology 347 3 444 454 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
salmon gonadotropin-releasing hormone;in situ hybridization;immunocytochemistry saumon de l'atlantique truite arc en ciel salmonidae salmo salar oncorhynchus mykiss poisson arn messager système nerveux central acide nucléique neuropeptide gnrh hybridation in situ méthode northern blot reproduction |
spellingShingle |
salmon gonadotropin-releasing hormone;in situ hybridization;immunocytochemistry saumon de l'atlantique truite arc en ciel salmonidae salmo salar oncorhynchus mykiss poisson arn messager système nerveux central acide nucléique neuropeptide gnrh hybridation in situ méthode northern blot reproduction Bailhache, Arazam, Klungland, Alestrom, Breton, B. Jego Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
topic_facet |
salmon gonadotropin-releasing hormone;in situ hybridization;immunocytochemistry saumon de l'atlantique truite arc en ciel salmonidae salmo salar oncorhynchus mykiss poisson arn messager système nerveux central acide nucléique neuropeptide gnrh hybridation in situ méthode northern blot reproduction |
description |
The decapeptide gonadotropin-releasing hormone (GnRH) is a key hormone for the central regulation of reproduction. The distribution of salmon GnRH (sGnRH), which is the major form in salmonids, has been studied in different fish species by immunocytochemistry. Discrepancies in data concerning the distribution of sGnRH perikarya led us to investigate this problem in two species, the Atlantic salmon and the rainbow trout, with in situ hybridization of sGnRH messenger, a highly specific molecular tool. By Northern blot analysis, the rainbow trout sGnRH messenger appears to be about 500 bases in length, which is close to those isolated from Atlantic salmon or masu salmon and characterized previously. In situ hybridization with riboprobes generated with Atlantic salmon sGnRH cDNA demonstrated that sGnRH perikarya are restricted to the ventral part of olfactory bulbs, telencephalon, and preoptic area. They are distributed on a nearly continuous line extending from the olfactory bulbs to the preoptic area in both salmonid species studied. Despite the presence of GnRH-like immunoreactivity in the preoptic magnocellular nucleus (NPOm) and in the tegmentum of the midbrain (MT), the sGnRH mRNA is not present in these two structures. Stained cells in NPOm could be target cells for GnRH and immunoreactive neurons in MT are likely to be chicken GnRH-II containing cells. Our study not only gives a precise distribution of the sGnRH system in two salmonids, Atlantic salmon and rainbow trout, but also clarifies the ambiguous data published up to now in rainbow trout |
format |
Article in Journal/Newspaper |
author |
Bailhache, Arazam, Klungland, Alestrom, Breton, B. Jego |
author_facet |
Bailhache, Arazam, Klungland, Alestrom, Breton, B. Jego |
author_sort |
Bailhache, |
title |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_short |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_full |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_fullStr |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_full_unstemmed |
Localization of salmon gonadotropin releasing hormone mRNA and peptide in brain of atlantic salmon and rainbow trout |
title_sort |
localization of salmon gonadotropin releasing hormone mrna and peptide in brain of atlantic salmon and rainbow trout |
publishDate |
1994 |
url |
http://prodinra.inra.fr/ft/79071C86-17B3-4AF5-8F2D-D667EFF024BD http://prodinra.inra.fr/record/110804 https://doi.org/10.1002/cne.903470310 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Comparative Neurology (347), 444-454. (1994) |
op_rights |
http://creativecommons.org/licenses/by-nd-nc/1.0/ |
op_rightsnorm |
CC-BY-ND-NC |
op_doi |
https://doi.org/10.1002/cne.903470310 |
container_title |
Journal of Comparative Neurology |
container_volume |
347 |
container_issue |
3 |
container_start_page |
444 |
op_container_end_page |
454 |
_version_ |
1766360420100079616 |