Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes
In order to elucidate the functional significance of accessory cells in freshwater fishes, such as the rainbow trout, which displays a poor adaptability to seawater life, a search for such cells was performed in two stenohaline freshwater fishes: the loach and the gudgeon. Accessory cells were never...
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Online Access: | http://prodinra.inra.fr/ft/55540D31-C079-4D70-9E56-3423EA2380E5 http://prodinra.inra.fr/record/104805 https://doi.org/10.1002/aja.1001870104 |
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ftinraparis:oai:prodinra.inra.fr:104805 2023-05-15T18:41:17+02:00 Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes Pisam, Boeuf, Prunet, Patrick Rambourg 1990 application/pdf http://prodinra.inra.fr/ft/55540D31-C079-4D70-9E56-3423EA2380E5 http://prodinra.inra.fr/record/104805 https://doi.org/10.1002/aja.1001870104 eng eng http://creativecommons.org/licenses/by-nd-nc/1.0/ CC-BY-ND-NC American Journal of Anatomy (187), 21-31. (1990) fish;gill;freswater;seawater;adaptatibility poisson mitochondrie salinité ultrastructure eau douce eau de mer branchie osmorégulation adaptation ARTICLE 1990 ftinraparis https://doi.org/10.1002/aja.1001870104 2018-02-06T23:24:42Z In order to elucidate the functional significance of accessory cells in freshwater fishes, such as the rainbow trout, which displays a poor adaptability to seawater life, a search for such cells was performed in two stenohaline freshwater fishes: the loach and the gudgeon. Accessory cells were never encountered in these species; but, in contrast, two types of chloride cells were observed consistently that strikingly resembled the alpha- and beta-cells previously described in the guppy, a freshwater-adapted euryhaline fish. The alpha-cell, a pale and elongated chloride cell, was located at the base of the secondary lamellae in close contact with the arterioarterial pillar capillary. Darker, ovoid chloride cells resembling the beta-cell were found exclusively in the interlamellar region of the primary epithelium facing the central venous sinous. The latter cells frequently formed multicellular complexes linked together by deep, narrow, apical junctions. In another experiment, a stenohaline seawater fish, the turbot, was adapted to diluted 5% saltwater and to fresh water. In seawater, the gill epithelium contained only one type of chloride cell, always associated with accessory cells. Due to numerous cytoplasmic interdigitations between the accessory cells and the apical portion of the chloride cell, there was a noticeable increase in the length of the shallow apical junction, sealing off the intercellular space between the two cell types. In 5% saltwater, there was a decrease in the number of these interdigitations and a concomitant decrease in the length of the shallow apical junction. In fresh water, chloride cells were partially or completely separated from the outside medium by modified accessory cells. It is thus concluded that accessory cells are found exclusively in fish living in seawater or preadapted to seawater and that they probably are involved in the formation and modulation of paracellular pathways for ionic excretion. In contrast, the respective roles of the two types of chloride cells observed in freshwater fishes are still to be determined. Article in Journal/Newspaper Turbot Institut National de la Recherche Agronomique: ProdINRA Pillar ENVELOPE(166.217,166.217,-77.583,-77.583) American Journal of Anatomy 187 1 21 31 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
fish;gill;freswater;seawater;adaptatibility poisson mitochondrie salinité ultrastructure eau douce eau de mer branchie osmorégulation adaptation |
spellingShingle |
fish;gill;freswater;seawater;adaptatibility poisson mitochondrie salinité ultrastructure eau douce eau de mer branchie osmorégulation adaptation Pisam, Boeuf, Prunet, Patrick Rambourg Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
topic_facet |
fish;gill;freswater;seawater;adaptatibility poisson mitochondrie salinité ultrastructure eau douce eau de mer branchie osmorégulation adaptation |
description |
In order to elucidate the functional significance of accessory cells in freshwater fishes, such as the rainbow trout, which displays a poor adaptability to seawater life, a search for such cells was performed in two stenohaline freshwater fishes: the loach and the gudgeon. Accessory cells were never encountered in these species; but, in contrast, two types of chloride cells were observed consistently that strikingly resembled the alpha- and beta-cells previously described in the guppy, a freshwater-adapted euryhaline fish. The alpha-cell, a pale and elongated chloride cell, was located at the base of the secondary lamellae in close contact with the arterioarterial pillar capillary. Darker, ovoid chloride cells resembling the beta-cell were found exclusively in the interlamellar region of the primary epithelium facing the central venous sinous. The latter cells frequently formed multicellular complexes linked together by deep, narrow, apical junctions. In another experiment, a stenohaline seawater fish, the turbot, was adapted to diluted 5% saltwater and to fresh water. In seawater, the gill epithelium contained only one type of chloride cell, always associated with accessory cells. Due to numerous cytoplasmic interdigitations between the accessory cells and the apical portion of the chloride cell, there was a noticeable increase in the length of the shallow apical junction, sealing off the intercellular space between the two cell types. In 5% saltwater, there was a decrease in the number of these interdigitations and a concomitant decrease in the length of the shallow apical junction. In fresh water, chloride cells were partially or completely separated from the outside medium by modified accessory cells. It is thus concluded that accessory cells are found exclusively in fish living in seawater or preadapted to seawater and that they probably are involved in the formation and modulation of paracellular pathways for ionic excretion. In contrast, the respective roles of the two types of chloride cells observed in freshwater fishes are still to be determined. |
format |
Article in Journal/Newspaper |
author |
Pisam, Boeuf, Prunet, Patrick Rambourg |
author_facet |
Pisam, Boeuf, Prunet, Patrick Rambourg |
author_sort |
Pisam, |
title |
Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
title_short |
Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
title_full |
Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
title_fullStr |
Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
title_full_unstemmed |
Ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
title_sort |
ultrastructural features of mitochondrial rich cells in stenohaline freshwater and seawater fishes |
publishDate |
1990 |
url |
http://prodinra.inra.fr/ft/55540D31-C079-4D70-9E56-3423EA2380E5 http://prodinra.inra.fr/record/104805 https://doi.org/10.1002/aja.1001870104 |
long_lat |
ENVELOPE(166.217,166.217,-77.583,-77.583) |
geographic |
Pillar |
geographic_facet |
Pillar |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
American Journal of Anatomy (187), 21-31. (1990) |
op_rights |
http://creativecommons.org/licenses/by-nd-nc/1.0/ |
op_rightsnorm |
CC-BY-ND-NC |
op_doi |
https://doi.org/10.1002/aja.1001870104 |
container_title |
American Journal of Anatomy |
container_volume |
187 |
container_issue |
1 |
container_start_page |
21 |
op_container_end_page |
31 |
_version_ |
1766230786927755264 |