The challenge to get MRI metabolic maps in fleshy fruit: tomato example

• Main Authors: Pagés, Guilhem, Deborde, Catherine, Lemaire-Chamley, Martine, Moing, Annick, Bonny, Jean-Marie
• Other Authors: Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INRAE, PROBE Research Infrastructure, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), BIBS - Plateforme Bioressources : Imagerie, Biochimie & Structure, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INRAE, PROBE Research Infrastructure, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaboHUB-Bordeaux, MetaboHUB, IB2019_GelSeed project of Inrae BAP Division
• Format: Conference Object
• Language: English
• Published: HAL CCSD 2024
• Subjects: [SDV]Life Sciences [q-bio]; Tromso
• Online Access: https://hal.science/hal-04683887; https://hal.science/hal-04683887/document; https://hal.science/hal-04683887/file/2024-Pages-resume%20poster%20MRPM.pdf

International audience Besides its classical water-related images, MRI is able to give metabolic information mainly thanks to two approaches. The first one is classical magnetic resonance spectroscopy imaging (MRSI) which leads to one NMR spectrum per image voxel. The second one is chemical exchange saturation transfer (CEST) contrast which gives a map weighed by the metabolite exchangeable protons. CEST MRI monitors the water signal intensity in function of the saturation frequency in a so-called z-spectrum. Saturating the signal at the frequency of exchangeable protons partially saturates the water signal leading to its decrease. Each contrast presents its own advantages and drawbacks. However, both methods have the same limitation, which concerns the excellent homogeneity of the magnetic field required over the entire image.