Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin
This study is part of a long-term partnership between Cotonou Autonomous Seaport, the Polytechnic School of Abomey-Calavi, the French Institute of Research for Sustainable Development, and the Tropical Neurology Institute (Inserm U1094, IRD U270 EpiMaCT, University of Limoges). We are grateful to La...
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Language: | English |
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HAL CCSD
2022
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Online Access: | https://hal.inrae.fr/hal-03957367 https://hal.inrae.fr/hal-03957367/document https://hal.inrae.fr/hal-03957367/file/parasite220072.pdf https://doi.org/10.1051/parasite/2022058 |
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ftinraparis:oai:HAL:hal-03957367v1 |
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openpolar |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
Toxoplasma gondii Small mammals Parasite ecology Infectious disease Molecular epidemiology Urban eco-epidemiology Benin [SDV]Life Sciences [q-bio] |
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Toxoplasma gondii Small mammals Parasite ecology Infectious disease Molecular epidemiology Urban eco-epidemiology Benin [SDV]Life Sciences [q-bio] Etougbétché, Jonas, R. Hamidović, Azra Dossou, Henri-Joël Coan-Grosso, Maeva Roques, Roxane Plault, Nicolas Houéménou, Gualbert Badou, Sylvestre Missihoun, Antoine, A. Abdou Karim, Issaka, Youssao Galal, Lokman Diagne, Christophe Dardé, Marie-Laure Dobigny, Gauthier Mercier, Aurélien Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin |
topic_facet |
Toxoplasma gondii Small mammals Parasite ecology Infectious disease Molecular epidemiology Urban eco-epidemiology Benin [SDV]Life Sciences [q-bio] |
description |
This study is part of a long-term partnership between Cotonou Autonomous Seaport, the Polytechnic School of Abomey-Calavi, the French Institute of Research for Sustainable Development, and the Tropical Neurology Institute (Inserm U1094, IRD U270 EpiMaCT, University of Limoges). We are grateful to Ladji, Agla and Saint-Jean authorities as well as inhabitants who kindly authorized us to access their households for trapping and interview purposes. We thank the Autonomous Port of Cotonou authorities and staff who facilitated our access to their infrastructures. We also thank the CBGP Small Mammal Collection (Centre de Biologie pour la Gestion des Populations, 2018, “CBGP – Small mammal Collection”, https://doi.org/10.15454/WWNUPO) for the conservation of samples from Benin. International audience Toxoplasmosis, one of the most prevalent parasitic infections in humans and animals, is caused by the intracellular protozoan parasite Toxoplasma gondii. Small mammals play a key role as intermediate reservoir hosts in the maintenance of the T. gondii life cycle. In this study, we estimated the molecular prevalence and provide genetic diversity data for T. gondii in 632 small mammals sampled in four areas of Cotonou city, Benin. Both the brain and heart of each individual were screened through T. gondii-targeting qPCR, and positive samples were then genotyped using a set of 15 T. gondii-specific microsatellites. Prevalence data were statistically analyzed in order to assess the relative impact of individual host characteristics, spatial distribution, composition of small mammal community, and urban landscape features. An overall T. gondii molecular prevalence of 15.2% was found and seven genotypes, all belonging to the Africa 1 lineage, could be retrieved from the invasive black rat Rattus rattus and the native African giant shrew Crocidura olivieri. Statistical analyses did not suggest any significant influence of the environmental parameters used in this study. Rather, depending on the local context, T. gondii ... |
author2 |
Ecole Polytechnique d'Abomey Calavi (EPAC) Université d’Abomey-Calavi = University of Abomey Calavi (UAC) Institut d'Epidémiologie Neurologique et de Neurologie Tropicale Centre de Biologie pour la Gestion des Populations (UMR CBGP) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC) (CNR Toxoplasmose-Toxoplasma BRC) CHU Limoges Unité Peste - Plague Unit Antananarivo, Madagascar Institut Pasteur de Madagascar Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) This work was supported by funds from the French Agence Nationale de la Recherche (ANR project IntroTox 17-CE35-0004 given to A. Mercier), the Nouvelle Aquitaine region of France, and recurrent funding (given to G. Dobigny) from the French Institute of Research for Sustainable Development (IRD). ANR-17-CE35-0004,IntroTox,PHENOMÈNES D'INTROGRESSIONS DANS L'ÉTUDE DE LA DIVERSITÉ GÉNÉTIQUE DU TOXOPLASME ENTRE LA FRANCE ET L'AFRIQUE DE L'OUEST ET CENTRALE : DES INFLUENCES HUMAINES ET ENVIRONEMENTALES(2017) |
format |
Article in Journal/Newspaper |
author |
Etougbétché, Jonas, R. Hamidović, Azra Dossou, Henri-Joël Coan-Grosso, Maeva Roques, Roxane Plault, Nicolas Houéménou, Gualbert Badou, Sylvestre Missihoun, Antoine, A. Abdou Karim, Issaka, Youssao Galal, Lokman Diagne, Christophe Dardé, Marie-Laure Dobigny, Gauthier Mercier, Aurélien |
author_facet |
Etougbétché, Jonas, R. Hamidović, Azra Dossou, Henri-Joël Coan-Grosso, Maeva Roques, Roxane Plault, Nicolas Houéménou, Gualbert Badou, Sylvestre Missihoun, Antoine, A. Abdou Karim, Issaka, Youssao Galal, Lokman Diagne, Christophe Dardé, Marie-Laure Dobigny, Gauthier Mercier, Aurélien |
author_sort |
Etougbétché, Jonas, R. |
title |
Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin |
title_short |
Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin |
title_full |
Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin |
title_fullStr |
Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin |
title_full_unstemmed |
Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin |
title_sort |
molecular prevalence, genetic characterization and patterns of toxoplasma gondii infection in domestic small mammals from cotonou, benin |
publisher |
HAL CCSD |
publishDate |
2022 |
url |
https://hal.inrae.fr/hal-03957367 https://hal.inrae.fr/hal-03957367/document https://hal.inrae.fr/hal-03957367/file/parasite220072.pdf https://doi.org/10.1051/parasite/2022058 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 1252-607X EISSN: 1776-1042 Parasite https://hal.inrae.fr/hal-03957367 Parasite, 2022, 29, pp.58. ⟨10.1051/parasite/2022058⟩ https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220072/parasite220072.html |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1051/parasite/2022058 info:eu-repo/semantics/altIdentifier/pmid/36562439 hal-03957367 https://hal.inrae.fr/hal-03957367 https://hal.inrae.fr/hal-03957367/document https://hal.inrae.fr/hal-03957367/file/parasite220072.pdf doi:10.1051/parasite/2022058 IRD: fdi:010086827 PUBMED: 36562439 WOS: 000901872900002 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1051/parasite/2022058 |
container_title |
Parasite |
container_volume |
29 |
container_start_page |
58 |
_version_ |
1811644228863787008 |
spelling |
ftinraparis:oai:HAL:hal-03957367v1 2024-09-30T14:41:49+00:00 Molecular prevalence, genetic characterization and patterns of Toxoplasma gondii infection in domestic small mammals from Cotonou, Benin Prévalence moléculaire, caractérisation génétique et schémas d’infection par Toxoplasma gondii chez les petits mammifères domestiques de Cotonou, Bénin Etougbétché, Jonas, R. Hamidović, Azra Dossou, Henri-Joël Coan-Grosso, Maeva Roques, Roxane Plault, Nicolas Houéménou, Gualbert Badou, Sylvestre Missihoun, Antoine, A. Abdou Karim, Issaka, Youssao Galal, Lokman Diagne, Christophe Dardé, Marie-Laure Dobigny, Gauthier Mercier, Aurélien Ecole Polytechnique d'Abomey Calavi (EPAC) Université d’Abomey-Calavi = University of Abomey Calavi (UAC) Institut d'Epidémiologie Neurologique et de Neurologie Tropicale Centre de Biologie pour la Gestion des Populations (UMR CBGP) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD France-Sud )-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM) Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC) (CNR Toxoplasmose-Toxoplasma BRC) CHU Limoges Unité Peste - Plague Unit Antananarivo, Madagascar Institut Pasteur de Madagascar Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP) This work was supported by funds from the French Agence Nationale de la Recherche (ANR project IntroTox 17-CE35-0004 given to A. Mercier), the Nouvelle Aquitaine region of France, and recurrent funding (given to G. Dobigny) from the French Institute of Research for Sustainable Development (IRD). ANR-17-CE35-0004,IntroTox,PHENOMÈNES D'INTROGRESSIONS DANS L'ÉTUDE DE LA DIVERSITÉ GÉNÉTIQUE DU TOXOPLASME ENTRE LA FRANCE ET L'AFRIQUE DE L'OUEST ET CENTRALE : DES INFLUENCES HUMAINES ET ENVIRONEMENTALES(2017) 2022-12-21 https://hal.inrae.fr/hal-03957367 https://hal.inrae.fr/hal-03957367/document https://hal.inrae.fr/hal-03957367/file/parasite220072.pdf https://doi.org/10.1051/parasite/2022058 en eng HAL CCSD EDP Sciences info:eu-repo/semantics/altIdentifier/doi/10.1051/parasite/2022058 info:eu-repo/semantics/altIdentifier/pmid/36562439 hal-03957367 https://hal.inrae.fr/hal-03957367 https://hal.inrae.fr/hal-03957367/document https://hal.inrae.fr/hal-03957367/file/parasite220072.pdf doi:10.1051/parasite/2022058 IRD: fdi:010086827 PUBMED: 36562439 WOS: 000901872900002 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1252-607X EISSN: 1776-1042 Parasite https://hal.inrae.fr/hal-03957367 Parasite, 2022, 29, pp.58. ⟨10.1051/parasite/2022058⟩ https://www.parasite-journal.org/articles/parasite/full_html/2022/01/parasite220072/parasite220072.html Toxoplasma gondii Small mammals Parasite ecology Infectious disease Molecular epidemiology Urban eco-epidemiology Benin [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2022 ftinraparis https://doi.org/10.1051/parasite/2022058 2024-09-17T14:54:42Z This study is part of a long-term partnership between Cotonou Autonomous Seaport, the Polytechnic School of Abomey-Calavi, the French Institute of Research for Sustainable Development, and the Tropical Neurology Institute (Inserm U1094, IRD U270 EpiMaCT, University of Limoges). We are grateful to Ladji, Agla and Saint-Jean authorities as well as inhabitants who kindly authorized us to access their households for trapping and interview purposes. We thank the Autonomous Port of Cotonou authorities and staff who facilitated our access to their infrastructures. We also thank the CBGP Small Mammal Collection (Centre de Biologie pour la Gestion des Populations, 2018, “CBGP – Small mammal Collection”, https://doi.org/10.15454/WWNUPO) for the conservation of samples from Benin. International audience Toxoplasmosis, one of the most prevalent parasitic infections in humans and animals, is caused by the intracellular protozoan parasite Toxoplasma gondii. Small mammals play a key role as intermediate reservoir hosts in the maintenance of the T. gondii life cycle. In this study, we estimated the molecular prevalence and provide genetic diversity data for T. gondii in 632 small mammals sampled in four areas of Cotonou city, Benin. Both the brain and heart of each individual were screened through T. gondii-targeting qPCR, and positive samples were then genotyped using a set of 15 T. gondii-specific microsatellites. Prevalence data were statistically analyzed in order to assess the relative impact of individual host characteristics, spatial distribution, composition of small mammal community, and urban landscape features. An overall T. gondii molecular prevalence of 15.2% was found and seven genotypes, all belonging to the Africa 1 lineage, could be retrieved from the invasive black rat Rattus rattus and the native African giant shrew Crocidura olivieri. Statistical analyses did not suggest any significant influence of the environmental parameters used in this study. Rather, depending on the local context, T. gondii ... Article in Journal/Newspaper Rattus rattus Institut National de la Recherche Agronomique: ProdINRA Parasite 29 58 |