Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms
International audience Chile is currently the second largest producer of farmed salmon worldwide, but Flavobacterium psychrophilum, as one of the most detrimental pathogens, is responsible for major losses during the freshwater culturing step in salmonid fish farms. An antigenic study conducted 10 y...
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Online Access: | https://hal.science/hal-02938415 https://hal.science/hal-02938415/document https://hal.science/hal-02938415/file/Duchaud.pdf https://doi.org/10.1111/jfd.13199 |
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ftinraparis:oai:HAL:hal-02938415v1 2024-09-09T19:30:48+00:00 Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms Avendaño-Herrera, Rubén E. Tapia-Cammas, Diana Duchaud, Éric Irgang, Rute Universidad Andrés Bello Santiago (UNAB) Interdisciplinary Center for Aquaculture Research Universidad de Concepción = University of Concepción Chile (UdeC) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ) Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Agencia de Innovación y Desarrollo de AndalucÃa, IDEA 15110027 This study was supported by the FONDAP 15110027 grant awarded by the Agencia Nacional de Investigaci?n y Desarrollo (ANID, Chile). The authors are grateful to the collaboration of the veterinary teams from the different salmonid farms. Special thanks are expressed to Professor Jean-Francois Bernardet for his aid. 2020 https://hal.science/hal-02938415 https://hal.science/hal-02938415/document https://hal.science/hal-02938415/file/Duchaud.pdf https://doi.org/10.1111/jfd.13199 en eng HAL CCSD Wiley info:eu-repo/semantics/altIdentifier/doi/10.1111/jfd.13199 info:eu-repo/semantics/altIdentifier/pmid/32567047 hal-02938415 https://hal.science/hal-02938415 https://hal.science/hal-02938415/document https://hal.science/hal-02938415/file/Duchaud.pdf doi:10.1111/jfd.13199 PUBMED: 32567047 WOS: 000541376000001 info:eu-repo/semantics/OpenAccess ISSN: 0140-7775 EISSN: 1365-2761 Journal of Fish Diseases https://hal.science/hal-02938415 Journal of Fish Diseases, 2020, 43 (8), pp.877-888. ⟨10.1111/jfd.13199⟩ Chilean salmon farms Flavobacterium psychrophilum serological diversity serotype PCR Updated serotyping for Chilean F. psychrophilum serological diversity [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2020 ftinraparis https://doi.org/10.1111/jfd.13199 2024-06-18T14:46:27Z International audience Chile is currently the second largest producer of farmed salmon worldwide, but Flavobacterium psychrophilum, as one of the most detrimental pathogens, is responsible for major losses during the freshwater culturing step in salmonid fish farms. An antigenic study conducted 10 years ago reported four serological groups using 20 F. psychrophilum Chilean strains. To reduce disease outbreaks and to develop vaccine candidates, antigenic knowledge needs to be regularly updated using a significant number of additional recent F. psychrophilum isolates. The present study aimed at investigating the serological diversity of 118 F. psychrophilum isolates collected between 2006 and 2018 from farmed Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch). The current study supports an expansion of the known antigenic groups in Chile from 4 to 14. However, the use of the slide-agglutination technique for serotyping is costly, is labour-intensive and requires significant technical expertise. Addressing these points, the mPCR-based procedure was a very useful tool for serotyping the collected Chilean F. psychrophilum isolates. This technique revealed the presence of diverse mPCR serotypes (i.e. types 0, 1, 2 and 4). Therefore, mPCR should be employed to select the bacterial strain(s) for vaccine development and to conduct follow-up, selective breeding or epidemiological surveillance in Chilean fish farms. Given the presented findings, changes to Chilean fish-farming practices are vital for ensuring the continued productivity and well-being of farmed salmonids. Article in Journal/Newspaper Atlantic salmon Salmo salar Institut National de la Recherche Agronomique: ProdINRA Journal of Fish Diseases 43 8 877 888 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
Chilean salmon farms Flavobacterium psychrophilum serological diversity serotype PCR Updated serotyping for Chilean F. psychrophilum serological diversity [SDV]Life Sciences [q-bio] |
spellingShingle |
Chilean salmon farms Flavobacterium psychrophilum serological diversity serotype PCR Updated serotyping for Chilean F. psychrophilum serological diversity [SDV]Life Sciences [q-bio] Avendaño-Herrera, Rubén E. Tapia-Cammas, Diana Duchaud, Éric Irgang, Rute Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms |
topic_facet |
Chilean salmon farms Flavobacterium psychrophilum serological diversity serotype PCR Updated serotyping for Chilean F. psychrophilum serological diversity [SDV]Life Sciences [q-bio] |
description |
International audience Chile is currently the second largest producer of farmed salmon worldwide, but Flavobacterium psychrophilum, as one of the most detrimental pathogens, is responsible for major losses during the freshwater culturing step in salmonid fish farms. An antigenic study conducted 10 years ago reported four serological groups using 20 F. psychrophilum Chilean strains. To reduce disease outbreaks and to develop vaccine candidates, antigenic knowledge needs to be regularly updated using a significant number of additional recent F. psychrophilum isolates. The present study aimed at investigating the serological diversity of 118 F. psychrophilum isolates collected between 2006 and 2018 from farmed Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss) and coho salmon (Oncorhynchus kisutch). The current study supports an expansion of the known antigenic groups in Chile from 4 to 14. However, the use of the slide-agglutination technique for serotyping is costly, is labour-intensive and requires significant technical expertise. Addressing these points, the mPCR-based procedure was a very useful tool for serotyping the collected Chilean F. psychrophilum isolates. This technique revealed the presence of diverse mPCR serotypes (i.e. types 0, 1, 2 and 4). Therefore, mPCR should be employed to select the bacterial strain(s) for vaccine development and to conduct follow-up, selective breeding or epidemiological surveillance in Chilean fish farms. Given the presented findings, changes to Chilean fish-farming practices are vital for ensuring the continued productivity and well-being of farmed salmonids. |
author2 |
Universidad Andrés Bello Santiago (UNAB) Interdisciplinary Center for Aquaculture Research Universidad de Concepción = University of Concepción Chile (UdeC) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ) Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Agencia de Innovación y Desarrollo de AndalucÃa, IDEA 15110027 This study was supported by the FONDAP 15110027 grant awarded by the Agencia Nacional de Investigaci?n y Desarrollo (ANID, Chile). The authors are grateful to the collaboration of the veterinary teams from the different salmonid farms. Special thanks are expressed to Professor Jean-Francois Bernardet for his aid. |
format |
Article in Journal/Newspaper |
author |
Avendaño-Herrera, Rubén E. Tapia-Cammas, Diana Duchaud, Éric Irgang, Rute |
author_facet |
Avendaño-Herrera, Rubén E. Tapia-Cammas, Diana Duchaud, Éric Irgang, Rute |
author_sort |
Avendaño-Herrera, Rubén E. |
title |
Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms |
title_short |
Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms |
title_full |
Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms |
title_fullStr |
Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms |
title_full_unstemmed |
Serological diversity in Flavobacterium psychrophilum: A critical update using isolates retrieved from Chilean salmon farms |
title_sort |
serological diversity in flavobacterium psychrophilum: a critical update using isolates retrieved from chilean salmon farms |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-02938415 https://hal.science/hal-02938415/document https://hal.science/hal-02938415/file/Duchaud.pdf https://doi.org/10.1111/jfd.13199 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
ISSN: 0140-7775 EISSN: 1365-2761 Journal of Fish Diseases https://hal.science/hal-02938415 Journal of Fish Diseases, 2020, 43 (8), pp.877-888. ⟨10.1111/jfd.13199⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1111/jfd.13199 info:eu-repo/semantics/altIdentifier/pmid/32567047 hal-02938415 https://hal.science/hal-02938415 https://hal.science/hal-02938415/document https://hal.science/hal-02938415/file/Duchaud.pdf doi:10.1111/jfd.13199 PUBMED: 32567047 WOS: 000541376000001 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1111/jfd.13199 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
8 |
container_start_page |
877 |
op_container_end_page |
888 |
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1809899780049993728 |