Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase
International audience AbstractNervous necrosis virus (NNV), Genus Betanodavirus, is the causative agent of viral encephalopathy and retinopathy (VER), a neuropathological disease that causes fish mortalities worldwide. The NNV genome is composed of two single-stranded RNA molecules, RNA1 and RNA2,...
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ftinraparis:oai:HAL:hal-02163084v1 2024-09-30T14:45:30+00:00 Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase Souto, Sandra Vázquez-Salgado, Lucía Olveira, José G. Bandín, Isabel Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Ministerio the Innovacion y Competitividad (Spain) AGL2014-54532-C2-2-R Xunta de Galicia (Spain)Xunta de Galicia ED431C 2018/18 FEDEREuropean Union (EU) 2019-12 https://hal.science/hal-02163084 https://hal.science/hal-02163084/document https://hal.science/hal-02163084/file/13567_2019_Article_669.pdf https://doi.org/10.1186/s13567-019-0669-4 en eng HAL CCSD BioMed Central info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-019-0669-4 info:eu-repo/semantics/altIdentifier/pmid/31227007 hal-02163084 https://hal.science/hal-02163084 https://hal.science/hal-02163084/document https://hal.science/hal-02163084/file/13567_2019_Article_669.pdf doi:10.1186/s13567-019-0669-4 PRODINRA: 485212 PUBMED: 31227007 WOS: 000472524700001 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 0928-4249 EISSN: 1297-9716 Veterinary Research https://hal.science/hal-02163084 Veterinary Research, 2019, 50 (1), pp.50. ⟨10.1186/s13567-019-0669-4⟩ atlantic halibut nodavirus viral encephalopathy in-vitro turbot localization virulence signals capsprotein infection replication [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2019 ftinraparis https://doi.org/10.1186/s13567-019-0669-4 2024-09-10T14:44:14Z International audience AbstractNervous necrosis virus (NNV), Genus Betanodavirus, is the causative agent of viral encephalopathy and retinopathy (VER), a neuropathological disease that causes fish mortalities worldwide. The NNV genome is composed of two single-stranded RNA molecules, RNA1 and RNA2, encoding the RNA polymerase and the coat protein, respectively. Betanodaviruses are classified into four genotypes: red-spotted grouper nervous necrosis virus (RGNNV), striped jack nervous necrosis virus (SJNNV), barfin flounder nervous necrosis virus (BFNNV) and tiger puffer nervous necrosis virus (TPNNV). In Southern Europe the presence of RGNNV, SJNNV and their natural reassortants (in both RNA1/RNA2 forms: RGNNV/SJNNV and SJNNV/RGNNV) has been reported. Pathology caused by these genotypes is closely linked to water temperature and the RNA1 segment encoding amino acids 1–445 has been postulated to regulate viral adaptation to temperature. Reassortants isolated from sole (RGNNV/SJNNV) show 6 substitutions in this region when compared with the RGNNV genotype (positions 41, 48, 218, 223, 238 and 289). We have demonstrated that change of these positions to those present in the RGNNV genotype cause low and delayed replication in vitro when compared with that of the wild type strain at 25 and 30 °C. The experimental infections confirmed the impact of the mutations on viral replication because at 25 °C the viral load and the mortality were significantly lower in fish infected with the mutant than in those challenged with the non-mutated virus. It was not possible to challenge fish at 30 °C because of the scarce tolerance of sole to this temperature. Article in Journal/Newspaper Turbot Institut National de la Recherche Agronomique: ProdINRA Veterinary Research 50 1 |
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Open Polar |
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Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
atlantic halibut nodavirus viral encephalopathy in-vitro turbot localization virulence signals capsprotein infection replication [SDV]Life Sciences [q-bio] |
spellingShingle |
atlantic halibut nodavirus viral encephalopathy in-vitro turbot localization virulence signals capsprotein infection replication [SDV]Life Sciences [q-bio] Souto, Sandra Vázquez-Salgado, Lucía Olveira, José G. Bandín, Isabel Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
topic_facet |
atlantic halibut nodavirus viral encephalopathy in-vitro turbot localization virulence signals capsprotein infection replication [SDV]Life Sciences [q-bio] |
description |
International audience AbstractNervous necrosis virus (NNV), Genus Betanodavirus, is the causative agent of viral encephalopathy and retinopathy (VER), a neuropathological disease that causes fish mortalities worldwide. The NNV genome is composed of two single-stranded RNA molecules, RNA1 and RNA2, encoding the RNA polymerase and the coat protein, respectively. Betanodaviruses are classified into four genotypes: red-spotted grouper nervous necrosis virus (RGNNV), striped jack nervous necrosis virus (SJNNV), barfin flounder nervous necrosis virus (BFNNV) and tiger puffer nervous necrosis virus (TPNNV). In Southern Europe the presence of RGNNV, SJNNV and their natural reassortants (in both RNA1/RNA2 forms: RGNNV/SJNNV and SJNNV/RGNNV) has been reported. Pathology caused by these genotypes is closely linked to water temperature and the RNA1 segment encoding amino acids 1–445 has been postulated to regulate viral adaptation to temperature. Reassortants isolated from sole (RGNNV/SJNNV) show 6 substitutions in this region when compared with the RGNNV genotype (positions 41, 48, 218, 223, 238 and 289). We have demonstrated that change of these positions to those present in the RGNNV genotype cause low and delayed replication in vitro when compared with that of the wild type strain at 25 and 30 °C. The experimental infections confirmed the impact of the mutations on viral replication because at 25 °C the viral load and the mortality were significantly lower in fish infected with the mutant than in those challenged with the non-mutated virus. It was not possible to challenge fish at 30 °C because of the scarce tolerance of sole to this temperature. |
author2 |
Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)) Institut National de la Recherche Agronomique (INRA) Ministerio the Innovacion y Competitividad (Spain) AGL2014-54532-C2-2-R Xunta de Galicia (Spain)Xunta de Galicia ED431C 2018/18 FEDEREuropean Union (EU) |
format |
Article in Journal/Newspaper |
author |
Souto, Sandra Vázquez-Salgado, Lucía Olveira, José G. Bandín, Isabel |
author_facet |
Souto, Sandra Vázquez-Salgado, Lucía Olveira, José G. Bandín, Isabel |
author_sort |
Souto, Sandra |
title |
Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
title_short |
Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
title_full |
Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
title_fullStr |
Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
title_full_unstemmed |
Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
title_sort |
amino acidic substitutions in the polymerase n-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase |
publisher |
HAL CCSD |
publishDate |
2019 |
url |
https://hal.science/hal-02163084 https://hal.science/hal-02163084/document https://hal.science/hal-02163084/file/13567_2019_Article_669.pdf https://doi.org/10.1186/s13567-019-0669-4 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
ISSN: 0928-4249 EISSN: 1297-9716 Veterinary Research https://hal.science/hal-02163084 Veterinary Research, 2019, 50 (1), pp.50. ⟨10.1186/s13567-019-0669-4⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13567-019-0669-4 info:eu-repo/semantics/altIdentifier/pmid/31227007 hal-02163084 https://hal.science/hal-02163084 https://hal.science/hal-02163084/document https://hal.science/hal-02163084/file/13567_2019_Article_669.pdf doi:10.1186/s13567-019-0669-4 PRODINRA: 485212 PUBMED: 31227007 WOS: 000472524700001 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1186/s13567-019-0669-4 |
container_title |
Veterinary Research |
container_volume |
50 |
container_issue |
1 |
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1811646123774836736 |