ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport.
International audience Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the E...
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ftinraparis:oai:HAL:hal-00593947v1 2024-09-09T19:39:20+00:00 ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène Institut Jean-Pierre Bourgin (IJPB) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Ecophysiologie Végétale, Agronomie et Nutritions (EVA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA) Biochimie et Physiologie Moléculaire des Plantes (BPMP) Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) 2011 https://hal.science/hal-00593947 https://hal.science/hal-00593947/document https://hal.science/hal-00593947/file/41793_20110818101442454_1.pdf https://doi.org/10.1371/journal.pone.0016645 en eng HAL CCSD Public Library of Science info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0016645 info:eu-repo/semantics/altIdentifier/pmid/21408051 hal-00593947 https://hal.science/hal-00593947 https://hal.science/hal-00593947/document https://hal.science/hal-00593947/file/41793_20110818101442454_1.pdf doi:10.1371/journal.pone.0016645 PRODINRA: 41793 PUBMED: 21408051 WOS: 000286835000004 info:eu-repo/semantics/OpenAccess ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-00593947 PLoS ONE, 2011, 6 (2), pp.e16645. ⟨10.1371/journal.pone.0016645⟩ Water abscisic acid water stress membrane transport root stomata aquaporin xylem [SDV.BV]Life Sciences [q-bio]/Vegetal Biology info:eu-repo/semantics/article Journal articles 2011 ftinraparis https://doi.org/10.1371/journal.pone.0016645 2024-07-09T14:19:43Z International audience Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components. Article in Journal/Newspaper eskimo* Institut National de la Recherche Agronomique: ProdINRA PLoS ONE 6 2 e16645 |
institution |
Open Polar |
collection |
Institut National de la Recherche Agronomique: ProdINRA |
op_collection_id |
ftinraparis |
language |
English |
topic |
Water abscisic acid water stress membrane transport root stomata aquaporin xylem [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
spellingShingle |
Water abscisic acid water stress membrane transport root stomata aquaporin xylem [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. |
topic_facet |
Water abscisic acid water stress membrane transport root stomata aquaporin xylem [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
description |
International audience Water economy in agricultural practices is an issue that is being addressed through studies aimed at understanding both plant water-use efficiency (WUE), i.e. biomass produced per water consumed, and responses to water shortage. In the model species Arabidopsis thaliana, the ESKIMO1 (ESK1) gene has been described as involved in freezing, cold and salt tolerance as well as in water economy: esk1 mutants have very low evapo-transpiration rates and high water-use efficiency. In order to establish ESK1 function, detailed characterization of esk1 mutants has been carried out. The stress hormone ABA (abscisic acid) was present at high levels in esk1 compared to wild type, nevertheless, the weak water loss of esk1 was independent of stomata closure through ABA biosynthesis, as combining mutant in this pathway with esk1 led to additive phenotypes. Measurement of root hydraulic conductivity suggests that the esk1 vegetative apparatus suffers water deficit due to a defect in water transport. ESK1 promoter-driven reporter gene expression was observed in xylem and fibers, the vascular tissue responsible for the transport of water and mineral nutrients from the soil to the shoots, via the roots. Moreover, in cross sections of hypocotyls, roots and stems, esk1 xylem vessels were collapsed. Finally, using Fourier-Transform Infrared (FTIR) spectroscopy, severe chemical modifications of xylem cell wall composition were highlighted in the esk1 mutants. Taken together our findings show that ESK1 is necessary for the production of functional xylem vessels, through its implication in the laying down of secondary cell wall components. |
author2 |
Institut Jean-Pierre Bourgin (IJPB) Institut National de la Recherche Agronomique (INRA)-AgroParisTech Ecophysiologie Végétale, Agronomie et Nutritions (EVA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA) Biochimie et Physiologie Moléculaire des Plantes (BPMP) Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) |
format |
Article in Journal/Newspaper |
author |
Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène |
author_facet |
Lefebvre, Valérie Fortabat, Marie-Noëlle Ducamp, Aloïse North, Helen M Maia-Grondard, Alessandra Trouverie, Jacques Boursiac, Yann Mouille, Gregory Durand-Tardif, Mylène |
author_sort |
Lefebvre, Valérie |
title |
ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. |
title_short |
ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. |
title_full |
ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. |
title_fullStr |
ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. |
title_full_unstemmed |
ESKIMO1 disruption in Arabidopsis alters vascular tissue and impairs water transport. |
title_sort |
eskimo1 disruption in arabidopsis alters vascular tissue and impairs water transport. |
publisher |
HAL CCSD |
publishDate |
2011 |
url |
https://hal.science/hal-00593947 https://hal.science/hal-00593947/document https://hal.science/hal-00593947/file/41793_20110818101442454_1.pdf https://doi.org/10.1371/journal.pone.0016645 |
genre |
eskimo* |
genre_facet |
eskimo* |
op_source |
ISSN: 1932-6203 EISSN: 1932-6203 PLoS ONE https://hal.science/hal-00593947 PLoS ONE, 2011, 6 (2), pp.e16645. ⟨10.1371/journal.pone.0016645⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0016645 info:eu-repo/semantics/altIdentifier/pmid/21408051 hal-00593947 https://hal.science/hal-00593947 https://hal.science/hal-00593947/document https://hal.science/hal-00593947/file/41793_20110818101442454_1.pdf doi:10.1371/journal.pone.0016645 PRODINRA: 41793 PUBMED: 21408051 WOS: 000286835000004 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1371/journal.pone.0016645 |
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PLoS ONE |
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6 |
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e16645 |
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