Effects of antioxidant agents against cyclosporine-induced
Background: To investigate the potential protective antioxidant role of ursodeoxycholic acid (UDCA), melatonin, and allopurinol treatment in cyclosporine (CsA)-induced hepatotoxicity. Methods: Hepatotoxicity was established in Sprague-Dawley rats by daily administration of CsA. Treatment groups were...
Main Authors: | , , , , , , , |
---|---|
Language: | unknown |
Published: |
2015
|
Subjects: | |
Online Access: | http://hdl.handle.net/11616/27062 |
id |
ftinonuuniv:oai:abakus.inonu.edu.tr:11616/27062 |
---|---|
record_format |
openpolar |
spelling |
ftinonuuniv:oai:abakus.inonu.edu.tr:11616/27062 2023-05-15T18:12:09+02:00 Effects of antioxidant agents against cyclosporine-induced hepatotoxicity Akbulut, S Elbe, H Eris, C Dogan, Z Toprak, G Yalcin, E Otan, E Turkoz, Y 2015 http://hdl.handle.net/11616/27062 unknown http://hdl.handle.net/11616/27062 JOURNAL OF SURGICAL RESEARCH 2015 ftinonuuniv 2022-03-28T19:52:09Z Background: To investigate the potential protective antioxidant role of ursodeoxycholic acid (UDCA), melatonin, and allopurinol treatment in cyclosporine (CsA)-induced hepatotoxicity. Methods: Hepatotoxicity was established in Sprague-Dawley rats by daily administration of CsA. Treatment groups were additionally administered UDCA, melatonin, or allopurinol treatments. Rats that received no CsA and no treatments served as a control group. Liver samples from each group were examined by histopathologic analysis to determine the effects of CsA treatment on liver morphology. Biochemical assays were also used to determine the effect of CsA treatment on liver function, in the presence or absence of UDCA, melatonin, or allopurinol. Results: CsA treatment induced hepatotoxicity, resulting in sinusoidal dilatation, congestion, infiltration, hydropic degeneration, and loss of glycogen storage in the liver. From a molecular perspective, the CsA treatment increased levels of malondialdehyde (MDA) levels, decreased levels of reduced glutathione and xanthine oxidase, and decreased activities of superoxide dismutase and catalase. The CsA treatment also resulted in decreased serum total antioxidant capacity, whereas alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin levels, and total oxidant status were increased. Treatment with UDCA, melatonin, or allopurinol reduced the CsA-induced histopathologic changes, as compared with CsA-treated samples. In addition, UDCA, melatonin, or allopurinol treatment mitigated the CsA-induced effects on glutathione and MDA levels, and on superoxide dismutase and catalase activities, as well as reduced the CsA-mediated perturbations in serum levels of total antioxidant capacity, total oxidant status, and alkaline phosphatase. Conclusions: UDCA, allopurinol, and melatonin may each help to protect against CsA-induced damage to liver tissues, possibly through effects on the antioxidant system. (C) 2015 Elsevier Inc. All rights reserved. C1 [Akbulut, Sami; Eris, Cengiz; Otan, Emrah] Inonu Univ, Fac Med, Dept Surg, TR-44280 Malatya, Turkey. [Akbulut, Sami; Eris, Cengiz; Otan, Emrah] Inonu Univ, Fac Med, Liver Transplant Inst, TR-44280 Malatya, Turkey. [Elbe, Hulya] Inonu Univ, Fac Med, Dept Histol & Embryol, TR-44280 Malatya, Turkey. [Dogan, Zumrut] Adiyaman Univ, Fac Med, Dept Anat, Adiyaman, Turkey. [Toprak, Gulten; Yalcin, Erhan] Dicle Univ, Fac Med, Dept Biochem, Diyarbakir, Turkey. [Turkoz, Yusuf] Inonu Univ, Fac Med, Dept Biochem, TR-44280 Malatya, Turkey. Other/Unknown Material sami Unknown |
institution |
Open Polar |
collection |
Unknown |
op_collection_id |
ftinonuuniv |
language |
unknown |
description |
Background: To investigate the potential protective antioxidant role of ursodeoxycholic acid (UDCA), melatonin, and allopurinol treatment in cyclosporine (CsA)-induced hepatotoxicity. Methods: Hepatotoxicity was established in Sprague-Dawley rats by daily administration of CsA. Treatment groups were additionally administered UDCA, melatonin, or allopurinol treatments. Rats that received no CsA and no treatments served as a control group. Liver samples from each group were examined by histopathologic analysis to determine the effects of CsA treatment on liver morphology. Biochemical assays were also used to determine the effect of CsA treatment on liver function, in the presence or absence of UDCA, melatonin, or allopurinol. Results: CsA treatment induced hepatotoxicity, resulting in sinusoidal dilatation, congestion, infiltration, hydropic degeneration, and loss of glycogen storage in the liver. From a molecular perspective, the CsA treatment increased levels of malondialdehyde (MDA) levels, decreased levels of reduced glutathione and xanthine oxidase, and decreased activities of superoxide dismutase and catalase. The CsA treatment also resulted in decreased serum total antioxidant capacity, whereas alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin levels, and total oxidant status were increased. Treatment with UDCA, melatonin, or allopurinol reduced the CsA-induced histopathologic changes, as compared with CsA-treated samples. In addition, UDCA, melatonin, or allopurinol treatment mitigated the CsA-induced effects on glutathione and MDA levels, and on superoxide dismutase and catalase activities, as well as reduced the CsA-mediated perturbations in serum levels of total antioxidant capacity, total oxidant status, and alkaline phosphatase. Conclusions: UDCA, allopurinol, and melatonin may each help to protect against CsA-induced damage to liver tissues, possibly through effects on the antioxidant system. (C) 2015 Elsevier Inc. All rights reserved. C1 [Akbulut, Sami; Eris, Cengiz; Otan, Emrah] Inonu Univ, Fac Med, Dept Surg, TR-44280 Malatya, Turkey. [Akbulut, Sami; Eris, Cengiz; Otan, Emrah] Inonu Univ, Fac Med, Liver Transplant Inst, TR-44280 Malatya, Turkey. [Elbe, Hulya] Inonu Univ, Fac Med, Dept Histol & Embryol, TR-44280 Malatya, Turkey. [Dogan, Zumrut] Adiyaman Univ, Fac Med, Dept Anat, Adiyaman, Turkey. [Toprak, Gulten; Yalcin, Erhan] Dicle Univ, Fac Med, Dept Biochem, Diyarbakir, Turkey. [Turkoz, Yusuf] Inonu Univ, Fac Med, Dept Biochem, TR-44280 Malatya, Turkey. |
author |
Akbulut, S Elbe, H Eris, C Dogan, Z Toprak, G Yalcin, E Otan, E Turkoz, Y |
spellingShingle |
Akbulut, S Elbe, H Eris, C Dogan, Z Toprak, G Yalcin, E Otan, E Turkoz, Y Effects of antioxidant agents against cyclosporine-induced |
author_facet |
Akbulut, S Elbe, H Eris, C Dogan, Z Toprak, G Yalcin, E Otan, E Turkoz, Y |
author_sort |
Akbulut, S |
title |
Effects of antioxidant agents against cyclosporine-induced |
title_short |
Effects of antioxidant agents against cyclosporine-induced |
title_full |
Effects of antioxidant agents against cyclosporine-induced |
title_fullStr |
Effects of antioxidant agents against cyclosporine-induced |
title_full_unstemmed |
Effects of antioxidant agents against cyclosporine-induced |
title_sort |
effects of antioxidant agents against cyclosporine-induced |
publishDate |
2015 |
url |
http://hdl.handle.net/11616/27062 |
genre |
sami |
genre_facet |
sami |
op_source |
JOURNAL OF SURGICAL RESEARCH |
op_relation |
http://hdl.handle.net/11616/27062 |
_version_ |
1766184712162770944 |