Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells
Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be viru...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Text |
Language: | unknown |
Published: |
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1111/1462-2920.14776 http://infoscience.epfl.ch/record/268685 |
id |
ftinfoscience:oai:infoscience.epfl.ch:268685 |
---|---|
record_format |
openpolar |
spelling |
ftinfoscience:oai:infoscience.epfl.ch:268685 2023-05-15T15:58:37+02:00 Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique 2019-08-08T09:36:09Z https://doi.org/10.1111/1462-2920.14776 http://infoscience.epfl.ch/record/268685 unknown doi:10.1111/1462-2920.14776 http://infoscience.epfl.ch/record/268685 http://infoscience.epfl.ch/record/268685 Text 2019 ftinfoscience https://doi.org/10.1111/1462-2920.14776 2023-02-13T22:53:10Z Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause to hemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams, respectively. We propose that hemocyte cytotoxicity, is a lethality trait shared by a broad range of mollusk pathogens and we speculate that T6SS was involved in parallel evolution of pathogen for mollusks. Text Crassostrea gigas Pacific oyster EPFL Infoscience (Ecole Polytechnique Fédérale Lausanne) Pacific Environmental Microbiology 22 10 4198 4211 |
institution |
Open Polar |
collection |
EPFL Infoscience (Ecole Polytechnique Fédérale Lausanne) |
op_collection_id |
ftinfoscience |
language |
unknown |
description |
Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are necessary for virulence but act independently to cause to hemocyte (oyster immune cell) cytotoxicity. A phylogenetically closely related T6SS was identified in V. aestuarianus and V. tapetis, which infect adult oysters and clams, respectively. We propose that hemocyte cytotoxicity, is a lethality trait shared by a broad range of mollusk pathogens and we speculate that T6SS was involved in parallel evolution of pathogen for mollusks. |
format |
Text |
author |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique |
spellingShingle |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
author_facet |
Piel, Damien Bruto, Maxime James, Adèle Labreuche, Yannick Lambert, Christophe Janicot, Adrian Chenivesse, Sabine Petton, Bruno Wegner, K. Mathias Stoudmann, Candice Blokesch, Melanie Le Roux, Frédérique |
author_sort |
Piel, Damien |
title |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_short |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_fullStr |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_full_unstemmed |
Selection of Vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
title_sort |
selection of vibrio crassostreae relies on a plasmid expressing a type 6 secretion system cytotoxic for host immune cells |
publishDate |
2019 |
url |
https://doi.org/10.1111/1462-2920.14776 http://infoscience.epfl.ch/record/268685 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
http://infoscience.epfl.ch/record/268685 |
op_relation |
doi:10.1111/1462-2920.14776 http://infoscience.epfl.ch/record/268685 |
op_doi |
https://doi.org/10.1111/1462-2920.14776 |
container_title |
Environmental Microbiology |
container_volume |
22 |
container_issue |
10 |
container_start_page |
4198 |
op_container_end_page |
4211 |
_version_ |
1766394389778661376 |