Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS
International audience Endocrine disrupting compounds (EDCs) are suspected to be responsible for many disorders of the human reproductive system. To establish a causality relationship between exposure to endocrine disruptors and disease, experiments on animals must be performed with improved or new...
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Online Access: | https://hal.science/hal-00727414 https://doi.org/10.1007/s00216-012-5723-2 |
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ftineris:oai:HAL:hal-00727414v1 2024-05-12T08:12:11+00:00 Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS Pouech, Charlène Tournier, Mikaël Quignot, Nadia Kiss, Agnèta Wiest, Laure Lafay, Florent Flament-Waton, Marie-Magdeleine Lemazurier, Emmanuel Cren-Olivé, Cécile ISA-TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé Institut des Sciences Analytiques (ISA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut National de l'Environnement Industriel et des Risques (INERIS) INERIS ANASTERAT 2012-03 https://hal.science/hal-00727414 https://doi.org/10.1007/s00216-012-5723-2 en eng HAL CCSD Springer Verlag info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-012-5723-2 hal-00727414 https://hal.science/hal-00727414 doi:10.1007/s00216-012-5723-2 ISSN: 1618-2642 EISSN: 1618-2650 Analytical and Bioanalytical Chemistry https://hal.science/hal-00727414 Analytical and Bioanalytical Chemistry, 2012, 402 (9), pp.2777-2788. ⟨10.1007/s00216-012-5723-2⟩ SERUM DERIVATIZATION VALIDATION METABOLITES TESTOSTERONE QUANTIFICATION QuEChERS Endocrine disruptors Hormones Testis CHROMATOGRAPHY-MASS-SPECTROMETRY Multi-residue LC-MS/MS PESTICIDES TISSUES TURBOT PSETTA-MAXIMA [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] info:eu-repo/semantics/article Journal articles 2012 ftineris https://doi.org/10.1007/s00216-012-5723-2 2024-04-17T23:48:11Z International audience Endocrine disrupting compounds (EDCs) are suspected to be responsible for many disorders of the human reproductive system. To establish a causality relationship between exposure to endocrine disruptors and disease, experiments on animals must be performed with improved or new analytical tools. Therefore, a simple, rapid, and effective multi-residue method was developed for the determination of four steroid hormones (i.e., testosterone, androstenedione, estrone, and estradiol), glucuronide and sulfate conjugates of estrone and estradiol and four endocrine disruptors in rat testis (i.e., bisphenol A, atrazine, and active metabolites of methoxychlor and vinclozolin). The sample preparation procedure was based on the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) approach. An analytical method was then developed to quantify these compounds at ultra-trace levels by liquid chromatography coupled to tandem mass spectrometry. The QuEChERS extraction was optimized with regard to the acetonitrile/water ratio used in the extraction step, the choice of the cleanup method and the acetonitrile/hexane ratio used in the cleanup step. The optimized extraction method exhibited recoveries between 89% and 108% for all tested compounds except the conjugates (31% to 58%). The detection limits of all compounds were below 20 ng g(-1) of wet weight of testis. The method was subsequently applied to determine the levels of hormones and EDCs in seven rat testis samples. Article in Journal/Newspaper Turbot INERIS: HAL (Institut National de l'Environnement Industriel et des Risques) Analytical and Bioanalytical Chemistry 402 9 2777 2788 |
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INERIS: HAL (Institut National de l'Environnement Industriel et des Risques) |
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ftineris |
language |
English |
topic |
SERUM DERIVATIZATION VALIDATION METABOLITES TESTOSTERONE QUANTIFICATION QuEChERS Endocrine disruptors Hormones Testis CHROMATOGRAPHY-MASS-SPECTROMETRY Multi-residue LC-MS/MS PESTICIDES TISSUES TURBOT PSETTA-MAXIMA [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] |
spellingShingle |
SERUM DERIVATIZATION VALIDATION METABOLITES TESTOSTERONE QUANTIFICATION QuEChERS Endocrine disruptors Hormones Testis CHROMATOGRAPHY-MASS-SPECTROMETRY Multi-residue LC-MS/MS PESTICIDES TISSUES TURBOT PSETTA-MAXIMA [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Pouech, Charlène Tournier, Mikaël Quignot, Nadia Kiss, Agnèta Wiest, Laure Lafay, Florent Flament-Waton, Marie-Magdeleine Lemazurier, Emmanuel Cren-Olivé, Cécile Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS |
topic_facet |
SERUM DERIVATIZATION VALIDATION METABOLITES TESTOSTERONE QUANTIFICATION QuEChERS Endocrine disruptors Hormones Testis CHROMATOGRAPHY-MASS-SPECTROMETRY Multi-residue LC-MS/MS PESTICIDES TISSUES TURBOT PSETTA-MAXIMA [CHIM.ANAL]Chemical Sciences/Analytical chemistry [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] |
description |
International audience Endocrine disrupting compounds (EDCs) are suspected to be responsible for many disorders of the human reproductive system. To establish a causality relationship between exposure to endocrine disruptors and disease, experiments on animals must be performed with improved or new analytical tools. Therefore, a simple, rapid, and effective multi-residue method was developed for the determination of four steroid hormones (i.e., testosterone, androstenedione, estrone, and estradiol), glucuronide and sulfate conjugates of estrone and estradiol and four endocrine disruptors in rat testis (i.e., bisphenol A, atrazine, and active metabolites of methoxychlor and vinclozolin). The sample preparation procedure was based on the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) approach. An analytical method was then developed to quantify these compounds at ultra-trace levels by liquid chromatography coupled to tandem mass spectrometry. The QuEChERS extraction was optimized with regard to the acetonitrile/water ratio used in the extraction step, the choice of the cleanup method and the acetonitrile/hexane ratio used in the cleanup step. The optimized extraction method exhibited recoveries between 89% and 108% for all tested compounds except the conjugates (31% to 58%). The detection limits of all compounds were below 20 ng g(-1) of wet weight of testis. The method was subsequently applied to determine the levels of hormones and EDCs in seven rat testis samples. |
author2 |
ISA-TRACES - Technologie et Recherche en Analyse Chimique pour l'Environnement et la Santé Institut des Sciences Analytiques (ISA) Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut National de l'Environnement Industriel et des Risques (INERIS) INERIS ANASTERAT |
format |
Article in Journal/Newspaper |
author |
Pouech, Charlène Tournier, Mikaël Quignot, Nadia Kiss, Agnèta Wiest, Laure Lafay, Florent Flament-Waton, Marie-Magdeleine Lemazurier, Emmanuel Cren-Olivé, Cécile |
author_facet |
Pouech, Charlène Tournier, Mikaël Quignot, Nadia Kiss, Agnèta Wiest, Laure Lafay, Florent Flament-Waton, Marie-Magdeleine Lemazurier, Emmanuel Cren-Olivé, Cécile |
author_sort |
Pouech, Charlène |
title |
Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS |
title_short |
Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS |
title_full |
Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS |
title_fullStr |
Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS |
title_full_unstemmed |
Multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by QuEChERS-based extraction and LC-MS/MS |
title_sort |
multi-residue analysis of free and conjugated hormones and endocrine disruptors in rat testis by quechers-based extraction and lc-ms/ms |
publisher |
HAL CCSD |
publishDate |
2012 |
url |
https://hal.science/hal-00727414 https://doi.org/10.1007/s00216-012-5723-2 |
genre |
Turbot |
genre_facet |
Turbot |
op_source |
ISSN: 1618-2642 EISSN: 1618-2650 Analytical and Bioanalytical Chemistry https://hal.science/hal-00727414 Analytical and Bioanalytical Chemistry, 2012, 402 (9), pp.2777-2788. ⟨10.1007/s00216-012-5723-2⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-012-5723-2 hal-00727414 https://hal.science/hal-00727414 doi:10.1007/s00216-012-5723-2 |
op_doi |
https://doi.org/10.1007/s00216-012-5723-2 |
container_title |
Analytical and Bioanalytical Chemistry |
container_volume |
402 |
container_issue |
9 |
container_start_page |
2777 |
op_container_end_page |
2788 |
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1798834479872081920 |