PCR-based sex determination of cetaceans and dugong from the Indian seas
Not Available A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the geno...
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ftindiancar:oai:krishi.icar.gov.in:123456789/62319 2023-05-15T15:36:22+02:00 PCR-based sex determination of cetaceans and dugong from the Indian seas Not Available Rajagopalan,M Anoop,B Jayasankar,P 2008 http://krishi.icar.gov.in/jspui/handle/123456789/62319 English eng Current Science Association Not Available http://krishi.icar.gov.in/jspui/handle/123456789/62319 Conservation marine mammals PCR- based sex determination vulnerable/endangered species. Article 2008 ftindiancar 2021-09-25T17:08:35Z Not Available A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act. Not Available Article in Journal/Newspaper Balaenoptera musculus Blue whale Sperm whale KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) Indian Pacific |
institution |
Open Polar |
collection |
KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
op_collection_id |
ftindiancar |
language |
English |
topic |
Conservation marine mammals PCR- based sex determination vulnerable/endangered species. |
spellingShingle |
Conservation marine mammals PCR- based sex determination vulnerable/endangered species. Rajagopalan,M Anoop,B Jayasankar,P PCR-based sex determination of cetaceans and dugong from the Indian seas |
topic_facet |
Conservation marine mammals PCR- based sex determination vulnerable/endangered species. |
description |
Not Available A sex-determination technique based on PCR amplifi- cation of genomic DNA extracted from the skin tissue has been standardized in cetaceans and dugong sam-pled from the Indian seas. A Y-chromosome-specific region (SRY or Sex-determining Y-chromosome gene) of 210–224 bp size in the genome has been amplified (only in males) using specific PCR primers. A fragment of the ZFX/ZFY (zinc finger protein genes located both on the X and Y chromosomes respectively) re-gion in the size range 442–445 bp is also amplified (in both sexes) using another pair of primers simultaneously as positive controls for confirmation of sex. Molecular sexing was standardized in spinner dolphin (Stenella longirostris), bridled dolphin (Stenella attenuata), bottlenose dolphin (Tursiops aduncus), Indo-Pacific humpbacked dolphin (Sousa chinensis), Risso’s dolphin (Grampus griseus), finless porpoise (Neopho-caena phocaenoides), sperm whale (Physeter macro-cephalus), blue whale (Balaenoptera musculus), Bryde’s whale (Balaenoptera edeni) and dugong (Dugong du-gon), which are all vulnerable/endangered species pro- tected under the Indian Wildlife Act. Not Available |
format |
Article in Journal/Newspaper |
author |
Rajagopalan,M Anoop,B Jayasankar,P |
author_facet |
Rajagopalan,M Anoop,B Jayasankar,P |
author_sort |
Rajagopalan,M |
title |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_short |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_full |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_fullStr |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_full_unstemmed |
PCR-based sex determination of cetaceans and dugong from the Indian seas |
title_sort |
pcr-based sex determination of cetaceans and dugong from the indian seas |
publisher |
Current Science Association |
publishDate |
2008 |
url |
http://krishi.icar.gov.in/jspui/handle/123456789/62319 |
geographic |
Indian Pacific |
geographic_facet |
Indian Pacific |
genre |
Balaenoptera musculus Blue whale Sperm whale |
genre_facet |
Balaenoptera musculus Blue whale Sperm whale |
op_relation |
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/62319 |
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1766366714561298432 |