Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India
Not Available Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South-East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespi...
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ftindiancar:oai:krishi.icar.gov.in:123456789/61900 2023-05-15T18:41:15+02:00 Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India Journal of Fish Diseases T. Raja Swaminathan Sundar Raj Nithianantham Preena Prasannan Geetha P. K. Pradhan Neeraj Sood Rahul G Kuamr Arun Sudhager S Naresh Kumar Sood 2021-8-1 http://krishi.icar.gov.in/jspui/handle/123456789/61900 English eng Not Available Swaminathan TR, Raj NS, Preena PG, Pradhan PK, Sood N, Kumar RG, Sudhagar A, Sood NK. Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India. J Fish Dis. 2021 Aug 27. doi:10.1111/jfd.13519. Not Available 0140-7775 http://krishi.icar.gov.in/jspui/handle/123456789/61900 Megalocytivirus,Osphronemus goramy,infectious spleen and kidney necrosis virus,electron microscopy Research Paper 2021 ftindiancar https://doi.org/10.1111/jfd.13519 2022-01-15T18:13:50Z Not Available Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South-East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV) and scale drop disease virus (SDDV). ISKNV-mediated infectious spleen and kidney necrosis disease (ISKND) is a major viral disease in both freshwater and marine fish species. In this study, we report the isolation of ISKNV from diseased giant gourami, Osphronemus goramy, in India. Transmission electron microscopy of ultrathin sections of kidney and spleen revealed the presence of numerous polygonal naked viral particles having an outer nucleocapsid layer within the cytoplasm of enlarged cells (115-125 nm). Molecular and phylogenetic analyses confirmed the presence of ISKNV and the major capsid protein (MCP) (1,362 bp) gene in the infected fish had a high similarity to the other ISKNV-I isolates. Moreover, ISKNV was propagated in the Astronotus ocellatus fin (AOF) cell line and further confirmed genotypically. A high mortality rate (60%) was observed in gourami fish injected with ISKNV-positive tissue homogenate through challenge studies. Considering the lethal nature of ISKNV, the present study spotlights the implementation of stringent biosecurity practices for the proper control of the disease in the country. Report Turbot KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) Journal of Fish Diseases |
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Open Polar |
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KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
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ftindiancar |
language |
English |
topic |
Megalocytivirus,Osphronemus goramy,infectious spleen and kidney necrosis virus,electron microscopy |
spellingShingle |
Megalocytivirus,Osphronemus goramy,infectious spleen and kidney necrosis virus,electron microscopy T. Raja Swaminathan Sundar Raj Nithianantham Preena Prasannan Geetha P. K. Pradhan Neeraj Sood Rahul G Kuamr Arun Sudhager S Naresh Kumar Sood Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India |
topic_facet |
Megalocytivirus,Osphronemus goramy,infectious spleen and kidney necrosis virus,electron microscopy |
description |
Not Available Megalocytivirus cause diseases that have serious economic impacts on aquaculture, mainly in East and South-East Asia. Five primary genotypes are known: infectious spleen and kidney necrosis virus (ISKNV), red sea bream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), threespine stickleback iridovirus (TSIV) and scale drop disease virus (SDDV). ISKNV-mediated infectious spleen and kidney necrosis disease (ISKND) is a major viral disease in both freshwater and marine fish species. In this study, we report the isolation of ISKNV from diseased giant gourami, Osphronemus goramy, in India. Transmission electron microscopy of ultrathin sections of kidney and spleen revealed the presence of numerous polygonal naked viral particles having an outer nucleocapsid layer within the cytoplasm of enlarged cells (115-125 nm). Molecular and phylogenetic analyses confirmed the presence of ISKNV and the major capsid protein (MCP) (1,362 bp) gene in the infected fish had a high similarity to the other ISKNV-I isolates. Moreover, ISKNV was propagated in the Astronotus ocellatus fin (AOF) cell line and further confirmed genotypically. A high mortality rate (60%) was observed in gourami fish injected with ISKNV-positive tissue homogenate through challenge studies. Considering the lethal nature of ISKNV, the present study spotlights the implementation of stringent biosecurity practices for the proper control of the disease in the country. |
format |
Report |
author |
T. Raja Swaminathan Sundar Raj Nithianantham Preena Prasannan Geetha P. K. Pradhan Neeraj Sood Rahul G Kuamr Arun Sudhager S Naresh Kumar Sood |
author_facet |
T. Raja Swaminathan Sundar Raj Nithianantham Preena Prasannan Geetha P. K. Pradhan Neeraj Sood Rahul G Kuamr Arun Sudhager S Naresh Kumar Sood |
author_sort |
T. Raja Swaminathan |
title |
Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India |
title_short |
Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India |
title_full |
Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India |
title_fullStr |
Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India |
title_full_unstemmed |
Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India |
title_sort |
infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, osphronemus goramy, in india |
publisher |
Not Available |
publishDate |
2021 |
url |
http://krishi.icar.gov.in/jspui/handle/123456789/61900 |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
Swaminathan TR, Raj NS, Preena PG, Pradhan PK, Sood N, Kumar RG, Sudhagar A, Sood NK. Infectious spleen and kidney necrosis virus-associated large-scale mortality in farmed giant gourami, Osphronemus goramy, in India. J Fish Dis. 2021 Aug 27. doi:10.1111/jfd.13519. Not Available 0140-7775 http://krishi.icar.gov.in/jspui/handle/123456789/61900 |
op_doi |
https://doi.org/10.1111/jfd.13519 |
container_title |
Journal of Fish Diseases |
_version_ |
1766230742134685696 |