Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius)
Not Available Cellular interleukin-10 (IL-10) gene from the peripheral blood mononuclear cells of the healthy Dromedary camel (Camelus dromedarius) and viral IL-10 (vIL-10) from the skin scabs of the Dromedary camels infected with contagious ecthyma (a parapoxviral infection in the camels) were ampl...
Main Authors: | , , , , , , |
---|---|
Format: | Report |
Language: | English |
Published: |
Elsevier
2013
|
Subjects: | |
Online Access: | http://krishi.icar.gov.in/jspui/handle/123456789/39975 |
id |
ftindiancar:oai:krishi.icar.gov.in:123456789/39975 |
---|---|
record_format |
openpolar |
spelling |
ftindiancar:oai:krishi.icar.gov.in:123456789/39975 2023-05-15T18:04:22+02:00 Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) Not Available G. Nagarajan Shelesh Kumar Swami Shyam Singh Dahiya G. Sivakumar S.D. Narnaware F.C. Tuteja N.V. Patil 2013-01-08 http://krishi.icar.gov.in/jspui/handle/123456789/39975 English eng Elsevier Not Available; Not Available http://krishi.icar.gov.in/jspui/handle/123456789/39975 Pseudocowpoxvirus Interleukin-10 Camel Research Paper 2013 ftindiancar 2021-02-18T12:27:30Z Not Available Cellular interleukin-10 (IL-10) gene from the peripheral blood mononuclear cells of the healthy Dromedary camel (Camelus dromedarius) and viral IL-10 (vIL-10) from the skin scabs of the Dromedary camels infected with contagious ecthyma (a parapoxviral infection in the camels) were amplified by polymerase chain reaction, cloned and characterized. Sequence analysis revealed that the open reading frame (ORF) of dromedarian camel IL-10 is 537 bp in length, encoding 178 amino acid polypeptide while open reading frame of vIL-10 from camel is 561 bp, encoding 187 amino acid polypeptide. The Dromedary camel IL-10 exhibited 62.6% and 68.5% sequence identity at the nucleotide and amino acid level, respectively, with vIL-10 from camel. Sequence analysis also revealed that the Dromedary camel IL-10 shared 99.4% and 98.3% identity at the nucleotide and amino acid level, respectively, with the Bactrian camel (Camelus bactrianus). But vIL-10 from camel shared 84.7% and 83.4% sequence identity at the nucleotide and amino acid level, respectively, with vIL-10 from reindeer (Rangifer tarandus), which is a ruminant species belonging to the order Artiodactyla. The present study was conducted to evaluate the evolutionary origin of the camel parapoxvirus with parapoxviruses of cattle and sheep and the resultant sequence analysis revealed that camel parapoxvirus is closely related to cattle parapoxvirus than sheep parapoxvirus (Orf virus). Not Available Report Rangifer tarandus KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) Dromedary ENVELOPE(163.033,163.033,-78.317,-78.317) |
institution |
Open Polar |
collection |
KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
op_collection_id |
ftindiancar |
language |
English |
topic |
Pseudocowpoxvirus Interleukin-10 Camel |
spellingShingle |
Pseudocowpoxvirus Interleukin-10 Camel G. Nagarajan Shelesh Kumar Swami Shyam Singh Dahiya G. Sivakumar S.D. Narnaware F.C. Tuteja N.V. Patil Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) |
topic_facet |
Pseudocowpoxvirus Interleukin-10 Camel |
description |
Not Available Cellular interleukin-10 (IL-10) gene from the peripheral blood mononuclear cells of the healthy Dromedary camel (Camelus dromedarius) and viral IL-10 (vIL-10) from the skin scabs of the Dromedary camels infected with contagious ecthyma (a parapoxviral infection in the camels) were amplified by polymerase chain reaction, cloned and characterized. Sequence analysis revealed that the open reading frame (ORF) of dromedarian camel IL-10 is 537 bp in length, encoding 178 amino acid polypeptide while open reading frame of vIL-10 from camel is 561 bp, encoding 187 amino acid polypeptide. The Dromedary camel IL-10 exhibited 62.6% and 68.5% sequence identity at the nucleotide and amino acid level, respectively, with vIL-10 from camel. Sequence analysis also revealed that the Dromedary camel IL-10 shared 99.4% and 98.3% identity at the nucleotide and amino acid level, respectively, with the Bactrian camel (Camelus bactrianus). But vIL-10 from camel shared 84.7% and 83.4% sequence identity at the nucleotide and amino acid level, respectively, with vIL-10 from reindeer (Rangifer tarandus), which is a ruminant species belonging to the order Artiodactyla. The present study was conducted to evaluate the evolutionary origin of the camel parapoxvirus with parapoxviruses of cattle and sheep and the resultant sequence analysis revealed that camel parapoxvirus is closely related to cattle parapoxvirus than sheep parapoxvirus (Orf virus). Not Available |
format |
Report |
author |
G. Nagarajan Shelesh Kumar Swami Shyam Singh Dahiya G. Sivakumar S.D. Narnaware F.C. Tuteja N.V. Patil |
author_facet |
G. Nagarajan Shelesh Kumar Swami Shyam Singh Dahiya G. Sivakumar S.D. Narnaware F.C. Tuteja N.V. Patil |
author_sort |
G. Nagarajan |
title |
Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) |
title_short |
Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) |
title_full |
Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) |
title_fullStr |
Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) |
title_full_unstemmed |
Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius) |
title_sort |
comparison of virokine from camel pseudocowpoxvirus (pcpv) with interleukin 10 of the dromedary camel (camelus dromedarius) |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://krishi.icar.gov.in/jspui/handle/123456789/39975 |
long_lat |
ENVELOPE(163.033,163.033,-78.317,-78.317) |
geographic |
Dromedary |
geographic_facet |
Dromedary |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_relation |
Not Available; Not Available http://krishi.icar.gov.in/jspui/handle/123456789/39975 |
_version_ |
1766175736565071872 |