Development and characterization of a cell line WAF from freshwater shark Wallago attu.
Not Available A new epithelial cell line, WAF was developed from caudal fin of freshwater shark, Wallago attu. The cell line was optimally maintained at 28 _C in Leibovitz-15 (L-15) medium supplemented with 20 % fetal bovine serum. The cell line was characterized by various cytogenetic and molecular...
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ftindiancar:oai:krishi.icar.gov.in:123456789/3483 2023-05-15T15:33:27+02:00 Development and characterization of a cell line WAF from freshwater shark Wallago attu. Not Available Akhilesh Dubey Mukunda Goswami Kamalendra Yadav Bhagwati S. Sharma 2014-01-01 http://krishi.icar.gov.in/jspui/handle/123456789/3483 English eng Springer Not Available; Dubey A, Goswami M, Yadav K, Sharma BS (2014). Development and characterization of a Cell line WAF from freshwater shark Wallago attu. Molecular Biology Reports. 41(2):915-24. http://krishi.icar.gov.in/jspui/handle/123456789/3483 Wallago attu Cell line Freshwater shark Characterization Research Paper 2014 ftindiancar 2022-03-19T18:21:24Z Not Available A new epithelial cell line, WAF was developed from caudal fin of freshwater shark, Wallago attu. The cell line was optimally maintained at 28 _C in Leibovitz-15 (L-15) medium supplemented with 20 % fetal bovine serum. The cell line was characterized by various cytogenetic and molecular markers. The cytogenetic analysis revealed a diploid count of 86 chromosomes at different passages. The origin of the cell lines was confirmed by the amplification of 547 and 654 bp sequences of 16S rRNA and cytochrome oxidase subunit I genes of mitochondrial DNA, respectively. WAF cells were characterized for their growth characteristics at different temperature and serum concentration. Epithelial morphology of the cell line was confirmed using immunocytochemistry. Further cell plating efficiency, transfection efficiency and viability of cryopreserved WAF cells was also determined. ytotoxicity and genotoxicity assessment of cadmium salts on WAF cells by MTT, NR and comet assay illustrated the utility of this cell line as an in vitro model for aquatic toxicological studies. The cell line will be further useful for studying oxidative stress markers against aquatic pollutants. Department of Biotechnology, Government of India Report Attu KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
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KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
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ftindiancar |
language |
English |
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Wallago attu Cell line Freshwater shark Characterization |
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Wallago attu Cell line Freshwater shark Characterization Akhilesh Dubey Mukunda Goswami Kamalendra Yadav Bhagwati S. Sharma Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
topic_facet |
Wallago attu Cell line Freshwater shark Characterization |
description |
Not Available A new epithelial cell line, WAF was developed from caudal fin of freshwater shark, Wallago attu. The cell line was optimally maintained at 28 _C in Leibovitz-15 (L-15) medium supplemented with 20 % fetal bovine serum. The cell line was characterized by various cytogenetic and molecular markers. The cytogenetic analysis revealed a diploid count of 86 chromosomes at different passages. The origin of the cell lines was confirmed by the amplification of 547 and 654 bp sequences of 16S rRNA and cytochrome oxidase subunit I genes of mitochondrial DNA, respectively. WAF cells were characterized for their growth characteristics at different temperature and serum concentration. Epithelial morphology of the cell line was confirmed using immunocytochemistry. Further cell plating efficiency, transfection efficiency and viability of cryopreserved WAF cells was also determined. ytotoxicity and genotoxicity assessment of cadmium salts on WAF cells by MTT, NR and comet assay illustrated the utility of this cell line as an in vitro model for aquatic toxicological studies. The cell line will be further useful for studying oxidative stress markers against aquatic pollutants. Department of Biotechnology, Government of India |
format |
Report |
author |
Akhilesh Dubey Mukunda Goswami Kamalendra Yadav Bhagwati S. Sharma |
author_facet |
Akhilesh Dubey Mukunda Goswami Kamalendra Yadav Bhagwati S. Sharma |
author_sort |
Akhilesh Dubey |
title |
Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
title_short |
Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
title_full |
Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
title_fullStr |
Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
title_full_unstemmed |
Development and characterization of a cell line WAF from freshwater shark Wallago attu. |
title_sort |
development and characterization of a cell line waf from freshwater shark wallago attu. |
publisher |
Springer |
publishDate |
2014 |
url |
http://krishi.icar.gov.in/jspui/handle/123456789/3483 |
genre |
Attu |
genre_facet |
Attu |
op_relation |
Not Available; Dubey A, Goswami M, Yadav K, Sharma BS (2014). Development and characterization of a Cell line WAF from freshwater shark Wallago attu. Molecular Biology Reports. 41(2):915-24. http://krishi.icar.gov.in/jspui/handle/123456789/3483 |
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1766364018742657024 |