The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens
Not Available Exposure to elevated temperature is an inherent feature of Atlantic cod (Gadus morhua) sea-cage culture in some regions (e.g., Newfoundland) and may also become an increasingly prevalent challenge for wild fish populations because of accelerated climate change. Therefore, understanding...
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ftindiancar:oai:krishi.icar.gov.in:123456789/22973 2023-05-15T15:26:44+02:00 The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens Not Available Tiago S. Hori A. Kurt Gamperl Gord Nash Marije Booman Ashoktaru Barat Matthew L. Rise 2013-01-01 http://krishi.icar.gov.in/jspui/handle/123456789/22973 English eng Genetics Society of Canada; NRC Research Press Not Available; 14 ISSN, 0008-4093 http://krishi.icar.gov.in/jspui/handle/123456789/22973 temperature Aeromonas salmonicida aquaculture Atlantic cod (Gadus morhua) microarray poly IC viral Research Paper 2013 ftindiancar 2022-03-19T18:23:04Z Not Available Exposure to elevated temperature is an inherent feature of Atlantic cod (Gadus morhua) sea-cage culture in some regions (e.g., Newfoundland) and may also become an increasingly prevalent challenge for wild fish populations because of accelerated climate change. Therefore, understanding how elevated temperatures impacts the immune response of this commercially important species may help to reduce the potential negative impacts of such challenges. Previously, we investigated the impacts of moderately elevated temperature on the antiviral responses of Atlantic cod (Hori et al. 2012) and reported that elevated temperature modulated the spleen transcriptome response to polyriboinosinic polyribocytidylic acid (pIC, a viral mimic). Herein, we report a complementary microarray study that investigated the impact of the same elevated temperature regime on the Atlantic cod spleen transcriptome response to intraperitoneal (IP) injection of formalin-killed Aeromonas salmonicida (ASAL). Fish were held at two different temperatures (10 °C and 16 °C) prior to immune stimulation and sampled 6 and 24 h post-injection (HPI). In this experiment, we identified 711 and 666 nonredundant ASAL-responsive genes at 6HPI and 24HPI, respectively. These included several known antibacterial genes, including hepcidin, cathelicidin, ferritin heavy subunit, and interleukin 8. However, we only identified 15 differentially expressed genes at 6HPI and 2 at 24HPI (FDR 1%) when comparing ASAL-injected fish held at 10 °C versus 16 °C. In contrast, the same comparisons with pIC-injected fish yielded 290 and 339 differentially expressed genes (FDR 1%) at 6HPI and 24HPI, respectively. These results suggest that moderately elevated temperature has a lesser effect on the Atlantic cod spleen transcriptome response to ASAL (i.e., the antibacterial response) than to pIC (i.e., antiviral response). Thus, the impacts of high temperatures on the cod’simmuneresponsemaybe pathogen dependent. Not Available Report atlantic cod Gadus morhua Newfoundland KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
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Open Polar |
collection |
KRISHI Publication and Data Inventory Repository (Knowledge based Resources Information Systems Hub for Innovations in Agriculture - Indian Council of Agricultural Research, ICAR) |
op_collection_id |
ftindiancar |
language |
English |
topic |
temperature Aeromonas salmonicida aquaculture Atlantic cod (Gadus morhua) microarray poly IC viral |
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temperature Aeromonas salmonicida aquaculture Atlantic cod (Gadus morhua) microarray poly IC viral Tiago S. Hori A. Kurt Gamperl Gord Nash Marije Booman Ashoktaru Barat Matthew L. Rise The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens |
topic_facet |
temperature Aeromonas salmonicida aquaculture Atlantic cod (Gadus morhua) microarray poly IC viral |
description |
Not Available Exposure to elevated temperature is an inherent feature of Atlantic cod (Gadus morhua) sea-cage culture in some regions (e.g., Newfoundland) and may also become an increasingly prevalent challenge for wild fish populations because of accelerated climate change. Therefore, understanding how elevated temperatures impacts the immune response of this commercially important species may help to reduce the potential negative impacts of such challenges. Previously, we investigated the impacts of moderately elevated temperature on the antiviral responses of Atlantic cod (Hori et al. 2012) and reported that elevated temperature modulated the spleen transcriptome response to polyriboinosinic polyribocytidylic acid (pIC, a viral mimic). Herein, we report a complementary microarray study that investigated the impact of the same elevated temperature regime on the Atlantic cod spleen transcriptome response to intraperitoneal (IP) injection of formalin-killed Aeromonas salmonicida (ASAL). Fish were held at two different temperatures (10 °C and 16 °C) prior to immune stimulation and sampled 6 and 24 h post-injection (HPI). In this experiment, we identified 711 and 666 nonredundant ASAL-responsive genes at 6HPI and 24HPI, respectively. These included several known antibacterial genes, including hepcidin, cathelicidin, ferritin heavy subunit, and interleukin 8. However, we only identified 15 differentially expressed genes at 6HPI and 2 at 24HPI (FDR 1%) when comparing ASAL-injected fish held at 10 °C versus 16 °C. In contrast, the same comparisons with pIC-injected fish yielded 290 and 339 differentially expressed genes (FDR 1%) at 6HPI and 24HPI, respectively. These results suggest that moderately elevated temperature has a lesser effect on the Atlantic cod spleen transcriptome response to ASAL (i.e., the antibacterial response) than to pIC (i.e., antiviral response). Thus, the impacts of high temperatures on the cod’simmuneresponsemaybe pathogen dependent. Not Available |
format |
Report |
author |
Tiago S. Hori A. Kurt Gamperl Gord Nash Marije Booman Ashoktaru Barat Matthew L. Rise |
author_facet |
Tiago S. Hori A. Kurt Gamperl Gord Nash Marije Booman Ashoktaru Barat Matthew L. Rise |
author_sort |
Tiago S. Hori |
title |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens |
title_short |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens |
title_full |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens |
title_fullStr |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens |
title_full_unstemmed |
The impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether Atlantic cod (Gadus morhua) are stimulated with bacterial versus viral antigens |
title_sort |
impact of a moderate chronic temperature increase on spleen immune-relevant gene transcription depends on whether atlantic cod (gadus morhua) are stimulated with bacterial versus viral antigens |
publisher |
Genetics Society of Canada; NRC Research Press |
publishDate |
2013 |
url |
http://krishi.icar.gov.in/jspui/handle/123456789/22973 |
genre |
atlantic cod Gadus morhua Newfoundland |
genre_facet |
atlantic cod Gadus morhua Newfoundland |
op_relation |
Not Available; 14 ISSN, 0008-4093 http://krishi.icar.gov.in/jspui/handle/123456789/22973 |
_version_ |
1766357215892996096 |