A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism b...
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Elsevier Science
1995
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ftindianacasci:oai:repository.ias.ac.in:88615 2023-05-15T18:45:35+02:00 A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. 1995-04-24 http://repository.ias.ac.in/88615/ http://www.sciencedirect.com/science/article/pii/037811199500087M unknown Elsevier Science Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. (1995) A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy Gene, 156 (2). pp. 223-227. ISSN 0378-1119 QH301 Biology Article PeerReviewed 1995 ftindianacasci 2013-01-20T13:33:37Z Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity. Article in Journal/Newspaper yellow poppy Indian Academy of Sciences: Publication of Fellows |
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Open Polar |
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Indian Academy of Sciences: Publication of Fellows |
op_collection_id |
ftindianacasci |
language |
unknown |
topic |
QH301 Biology |
spellingShingle |
QH301 Biology Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
topic_facet |
QH301 Biology |
description |
Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity. |
format |
Article in Journal/Newspaper |
author |
Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. |
author_facet |
Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. |
author_sort |
Sulaiman, I. M. |
title |
A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
title_short |
A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
title_full |
A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
title_fullStr |
A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
title_full_unstemmed |
A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy |
title_sort |
multicopy dna sequence from meconopsis simplicifolia discriminates between the different species of this endangered himalayan poppy |
publisher |
Elsevier Science |
publishDate |
1995 |
url |
http://repository.ias.ac.in/88615/ http://www.sciencedirect.com/science/article/pii/037811199500087M |
genre |
yellow poppy |
genre_facet |
yellow poppy |
op_relation |
Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. (1995) A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy Gene, 156 (2). pp. 223-227. ISSN 0378-1119 |
_version_ |
1766236680161853440 |