A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy

Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism b...

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Bibliographic Details
Main Authors: Sulaiman, I. M., Ehtesham, N. Z., Hasnain, S. E.
Format: Article in Journal/Newspaper
Language:unknown
Published: Elsevier Science 1995
Subjects:
QH301 Biology
yellow poppy
Online Access:http://repository.ias.ac.in/88615/
http://www.sciencedirect.com/science/article/pii/037811199500087M
id ftindianacasci:oai:repository.ias.ac.in:88615
record_format openpolar
spelling ftindianacasci:oai:repository.ias.ac.in:88615 2023-05-15T18:45:35+02:00 A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. 1995-04-24 http://repository.ias.ac.in/88615/ http://www.sciencedirect.com/science/article/pii/037811199500087M unknown Elsevier Science Sulaiman, I. M. Ehtesham, N. Z. Hasnain, S. E. (1995) A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy Gene, 156 (2). pp. 223-227. ISSN 0378-1119 QH301 Biology Article PeerReviewed 1995 ftindianacasci 2013-01-20T13:33:37Z Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity. Article in Journal/Newspaper yellow poppy Indian Academy of Sciences: Publication of Fellows
institution Open Polar
collection Indian Academy of Sciences: Publication of Fellows
op_collection_id ftindianacasci
language unknown
topic QH301 Biology
spellingShingle QH301 Biology
Sulaiman, I. M.
Ehtesham, N. Z.
Hasnain, S. E.
A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
topic_facet QH301 Biology
description Clones harboring multicopy DNA sequences were isolated on the basis of reverse genome hybridization to Meconopsis paniculata (Himalayan yellow poppy) DNA from a Sau3A partial genomic plasmid library of M. simplicifolia (Himalayan blue poppy). Restriction-endonuclease-dependent genetic polymorphism between five species of Meconopsis, M. aculeata, M. paniculata, M. simplicifolia, M. sinuata and M. villosa, belonging to geographically isolated populations, was evident in genomic DNA filter hybridizations when probed with a clone (pIMS10) isolated from this library. Pooled DNA from seedlings originating from plants of individual populations of M. paniculata, M. simplicifolia and M. villosa gave similar band patterns, with respect to a given enzyme, suggesting intra-population genetic homogeneity.
format Article in Journal/Newspaper
author Sulaiman, I. M.
Ehtesham, N. Z.
Hasnain, S. E.
author_facet Sulaiman, I. M.
Ehtesham, N. Z.
Hasnain, S. E.
author_sort Sulaiman, I. M.
title A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
title_short A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
title_full A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
title_fullStr A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
title_full_unstemmed A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy
title_sort multicopy dna sequence from meconopsis simplicifolia discriminates between the different species of this endangered himalayan poppy
publisher Elsevier Science
publishDate 1995
url http://repository.ias.ac.in/88615/
http://www.sciencedirect.com/science/article/pii/037811199500087M
genre yellow poppy
genre_facet yellow poppy
op_relation Sulaiman, I. M.
Ehtesham, N. Z.
Hasnain, S. E. (1995) A multicopy DNA sequence from Meconopsis simplicifolia discriminates between the different species of this endangered Himalayan poppy Gene, 156 (2). pp. 223-227. ISSN 0378-1119
_version_ 1766236680161853440

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