Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica
One anaerobic, proteolytic bacterium isolated from Schirmacher oasis, Antarctica was characterised taxonomically. Based on morphology, biochemical characteristics and 16S rRNA sequence the isolate was identified as Clostridium species with closest similarity with Clostridium subterminale. Isolate wa...
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ftindianacasci:oai:repository.ias.ac.in:64329 2023-05-15T13:46:14+02:00 Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica Alam, S. I. Dube, Smita Reddy, G. S. N. Bhattacharya, B. K. Shivaji, S. Singh, Lokendra 2005-04-01 http://repository.ias.ac.in/64329/ http://www.sciencedirect.com/science/article/pii/S014102290500013X unknown Elsevier Science Alam, S. I. Dube, Smita Reddy, G. S. N. Bhattacharya, B. K. Shivaji, S. Singh, Lokendra (2005) Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica Enzyme and Microbial Technology, 36 (5-6). pp. 824-831. ISSN 0141-0229 QL Zoology Article PeerReviewed 2005 ftindianacasci 2013-01-20T12:25:37Z One anaerobic, proteolytic bacterium isolated from Schirmacher oasis, Antarctica was characterised taxonomically. Based on morphology, biochemical characteristics and 16S rRNA sequence the isolate was identified as Clostridium species with closest similarity with Clostridium subterminale. Isolate was psychrotrophic forming maximum cell mass between 5 and 10 °C and produced extracellular protease. Growth was observed in the pH range of 6.5-8.5 with optimum at pH 8. Protease was purified 12.7-fold with a total yield of 26.2%. Effect of temperature, pH and salt concentration on enzyme activity were studied. Protease was found to be a serine-type metaloenzyme, which is active in a broad range of pH. It was moderately thermolabile and resistant to SDS. Enzyme kinetics revealed a tendency to decrease K m with increase in temperature for casein substrate. Article in Journal/Newspaper Antarc* Antarctica Indian Academy of Sciences: Publication of Fellows |
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Indian Academy of Sciences: Publication of Fellows |
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topic |
QL Zoology |
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QL Zoology Alam, S. I. Dube, Smita Reddy, G. S. N. Bhattacharya, B. K. Shivaji, S. Singh, Lokendra Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica |
topic_facet |
QL Zoology |
description |
One anaerobic, proteolytic bacterium isolated from Schirmacher oasis, Antarctica was characterised taxonomically. Based on morphology, biochemical characteristics and 16S rRNA sequence the isolate was identified as Clostridium species with closest similarity with Clostridium subterminale. Isolate was psychrotrophic forming maximum cell mass between 5 and 10 °C and produced extracellular protease. Growth was observed in the pH range of 6.5-8.5 with optimum at pH 8. Protease was purified 12.7-fold with a total yield of 26.2%. Effect of temperature, pH and salt concentration on enzyme activity were studied. Protease was found to be a serine-type metaloenzyme, which is active in a broad range of pH. It was moderately thermolabile and resistant to SDS. Enzyme kinetics revealed a tendency to decrease K m with increase in temperature for casein substrate. |
format |
Article in Journal/Newspaper |
author |
Alam, S. I. Dube, Smita Reddy, G. S. N. Bhattacharya, B. K. Shivaji, S. Singh, Lokendra |
author_facet |
Alam, S. I. Dube, Smita Reddy, G. S. N. Bhattacharya, B. K. Shivaji, S. Singh, Lokendra |
author_sort |
Alam, S. I. |
title |
Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica |
title_short |
Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica |
title_full |
Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica |
title_fullStr |
Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica |
title_full_unstemmed |
Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica |
title_sort |
purification and characterisation of extracellular protease produced by clostridium sp. from schirmacher oasis, antarctica |
publisher |
Elsevier Science |
publishDate |
2005 |
url |
http://repository.ias.ac.in/64329/ http://www.sciencedirect.com/science/article/pii/S014102290500013X |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Alam, S. I. Dube, Smita Reddy, G. S. N. Bhattacharya, B. K. Shivaji, S. Singh, Lokendra (2005) Purification and characterisation of extracellular protease produced by Clostridium sp. from Schirmacher oasis, Antarctica Enzyme and Microbial Technology, 36 (5-6). pp. 824-831. ISSN 0141-0229 |
_version_ |
1766238706698551296 |