Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae
Cytoplasmic proteins from the antarctic psychrotrophic bacterium Pseudomonas syringae showed two phosphorylated proteins of molecular mass 66 kDa and 62 kDa. The phosphorylation of 66 kDa protein was enhanced in the presence of Triton X-100 solubilised membrane proteins at a higher temperature (30°C...
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John Wiley and Sons
1994
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Online Access: | http://repository.ias.ac.in/64327/ http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1994.tb07142.x/abstract |
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ftindianacasci:oai:repository.ias.ac.in:64327 2023-05-15T13:46:14+02:00 Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae Ray, M. K. Seshu Kumar, G. Shivaji, S. 1994-09 http://repository.ias.ac.in/64327/ http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1994.tb07142.x/abstract unknown John Wiley and Sons Ray, M. K. Seshu Kumar, G. Shivaji, S. (1994) Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae FEMS Microbiology Letters, 122 (1-2). pp. 49-54. ISSN 0378-1097 QL Zoology Article PeerReviewed 1994 ftindianacasci 2013-01-20T12:25:37Z Cytoplasmic proteins from the antarctic psychrotrophic bacterium Pseudomonas syringae showed two phosphorylated proteins of molecular mass 66 kDa and 62 kDa. The phosphorylation of 66 kDa protein was enhanced in the presence of Triton X-100 solubilised membrane proteins at a higher temperature (30°C) only. Western blot analysis and phosphoamino acid analysis indicated that the 66 kDa protein is phosphorylated at a tyrosine residue. Surprisingly, sodium orthovanadate, which is a known phosphotyrosine phosphatase (PTPase) inhibitor, inhibited the phosphorylation of the protein. The possible importance of this tyrosine phosphorylated protein to growth at low temperature is suggested. Article in Journal/Newspaper Antarc* Antarctic Indian Academy of Sciences: Publication of Fellows Antarctic The Antarctic Triton ENVELOPE(-55.615,-55.615,49.517,49.517) |
institution |
Open Polar |
collection |
Indian Academy of Sciences: Publication of Fellows |
op_collection_id |
ftindianacasci |
language |
unknown |
topic |
QL Zoology |
spellingShingle |
QL Zoology Ray, M. K. Seshu Kumar, G. Shivaji, S. Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae |
topic_facet |
QL Zoology |
description |
Cytoplasmic proteins from the antarctic psychrotrophic bacterium Pseudomonas syringae showed two phosphorylated proteins of molecular mass 66 kDa and 62 kDa. The phosphorylation of 66 kDa protein was enhanced in the presence of Triton X-100 solubilised membrane proteins at a higher temperature (30°C) only. Western blot analysis and phosphoamino acid analysis indicated that the 66 kDa protein is phosphorylated at a tyrosine residue. Surprisingly, sodium orthovanadate, which is a known phosphotyrosine phosphatase (PTPase) inhibitor, inhibited the phosphorylation of the protein. The possible importance of this tyrosine phosphorylated protein to growth at low temperature is suggested. |
format |
Article in Journal/Newspaper |
author |
Ray, M. K. Seshu Kumar, G. Shivaji, S. |
author_facet |
Ray, M. K. Seshu Kumar, G. Shivaji, S. |
author_sort |
Ray, M. K. |
title |
Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae |
title_short |
Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae |
title_full |
Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae |
title_fullStr |
Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae |
title_full_unstemmed |
Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae |
title_sort |
tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium pseudomonas syringae |
publisher |
John Wiley and Sons |
publishDate |
1994 |
url |
http://repository.ias.ac.in/64327/ http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1994.tb07142.x/abstract |
long_lat |
ENVELOPE(-55.615,-55.615,49.517,49.517) |
geographic |
Antarctic The Antarctic Triton |
geographic_facet |
Antarctic The Antarctic Triton |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Ray, M. K. Seshu Kumar, G. Shivaji, S. (1994) Tyrosine phosphorylation of a cytoplasmic protein from the antarctic psychrotrophic bacterium Pseudomonas syringae FEMS Microbiology Letters, 122 (1-2). pp. 49-54. ISSN 0378-1097 |
_version_ |
1766238704784900096 |