Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands

• Main Authors: Shivaji, S., Gupta, Pratima, Chaturvedi, Preeti, Suresh, K., Delille, Daniel
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Society for General Microbiology 2005
• Subjects: QL Zoology; Kerguelen; Kerguelen Islands
• Online Access: http://repository.ias.ac.in/64281/; http://ijs.sgmjournals.org/content/55/4/1453.abstract?sid=c0a34976-4052-4bbd-b7d2-1d872ed089cd

A psychrotolerant, Gram-negative, motile bacterium, designated CK 47 T , was isolated from sea water off the subantarctic Kerguelen islands (50° 40' S 68° 25' E). The isolate grew optimally at 22°C and minimum and maximum temperature of growth were 4 and 37°C, respectively. It required Na + for growth and exhibited optimum growth at pH 8·5 and 4% NaCl. It utilized hexane, heptane and petroleum ether as sole sources of carbon. Strain CK 47 T had Q9 as the major respiratory quinone and C 16:0 (21·7%), C 17:0 (21·3%), C 18:0 (5·7%), C 18:1 ω7c (9·0%) and C 18:1 ω9c (31·4%) as predominant fatty acids. The G+C content of the DNA was 58 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that CK 47 T formed a coherent cluster within the genus Marinobacter. It exhibited highest 16S rRNA gene sequence similarity of 96·8% with Marinobacter lipolyticus. However, the level of DNA-DNA relatedness between strain CK47 T and M. lipolyticus was only 55%. On the basis of phenotypic characteristics, and phylogenetic and genotypic distinctiveness, strain CK 47 T is considered to represent a novel species of the genus Marinobacter. The name Marinobacter maritimus sp. nov. is proposed, with CK 47 T (=JCM 12521 T =MTCC 6519 T ) as the type strain.