Marinomonas polaris sp. nov., a psychrohalotolerant strain isolated from coastal sea water off the subantarctic Kerguelen islands

• Main Authors: Gupta, Pratima, Chaturvedi, Preeti, Pradhan, Suman, Delille, Daniel, Shivaji, Sisinthy
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Society for General Microbiology 2006
• Subjects: QL Zoology; Kerguelen; Kerguelen Islands; Kerguelen Island
• Online Access: http://repository.ias.ac.in/64277/; http://ijs.sgmjournals.org/content/56/2/361.abstract?sid=d2c82c26-071c-4e6c-9528-e121daa97f64

Two aerobic, psychrohalotolerant, motile bacterial isolates, CK13 T and CK16, isolated from sea-water samples collected off the subantarctic Kerguelen island, were characterized by using a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence data, the strains were 99·6% similar and exhibited 93-97% similarity with the seven recognized species of Marinomonas. The most closely related species were Marinomonas pontica and Marinomonas primoryensis, with 97 and 96% similarity at the 16S rRNA gene sequence level, respectively. DNA-DNA hybridization values between strain CK13 T and M. pontica and M. primoryensis were only 58 and 40%, respectively. The major fatty acids present in strain CK13 T were iso-C 16:0 , C 16:0 , C 16:1 ω7c and C 18:1 ω7c. The DNA G+C content of strain CK13T was 41·2 mol%. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. All the above characteristics support the affiliation of strain CK13 T to the genus Marinomonas. Phylogenetic analysis and phenotypic and genotypic distinctiveness confirmed that strains CK13 T and CK16 are members of a novel species of the genus Marinomonas, for which the name Marinomonas polaris sp. nov. is proposed. The type strain is CK13 T (=MTCC 6645 T =DSM 16579 T =JCM 12522 T ).