Process intensification by microwave irradiation in immobilized-lipase catalysis in solvent-free synthesis of ethyl valerate

• Published in: Molecular Catalysis
• Main Authors: Bhavsar, Kalpesh V., Yadav, Ganapati D.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Elsevier Science 2018
• Subjects: TP Chemical technology; Antarc*; Antarctica
• Online Access: http://repository.ias.ac.in/125380/; https://doi.org/10.1016/j.mcat.2018.09.019

Valeric esters are a promising energy source and used in different industries ranging from biofuels, perfumes, food, flavors to pharmaceuticals. Ethyl valerate is one of the esters possessing all these attributes as an industrial chemical. In the current work an enzymatic route was examined using valeric acid and ethanol with commercially available lipases, namely, Lipozyme RM IM, Lipozyme TL IM and Novozyme 435 (Candida antarctica lipase B) under solvent fee condition and microwave irradiation. Novozym 435 could lead to 69.2% conversion of valeric acid at 50 °C using equimolar ratio of valeric acid and ethanol at enzyme loading of 10.06 g L−1 in 40 min under microwave irradiation. Lineweaver-Burk plot shows ternary complex mechanism with inhibition by ethanol. A reaction mechanism is proposed and kinetic rate equation derived. The enzyme is reusable.