Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid
Isoniazid is a useful antitubercular drug widely employed in combination therapy with rifampicin. The synthesis of isoniazid from ethyl isonicotinate and hydrazine hydrate was studied in non-aqueous media via lipase-catalyzed hydrazinolysis under both conventional heating and microwave irradiation b...
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ftindianacasci:oai:repository.ias.ac.in:111662 2023-05-15T13:39:33+02:00 Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid Yadav, Ganapati D. Sajgure, Ashwini D. 2007-11 http://repository.ias.ac.in/111662/ http://onlinelibrary.wiley.com/doi/10.1002/jctb.1738/abstract unknown John Wiley & Sons, Inc Yadav, Ganapati D. Sajgure, Ashwini D. (2007) Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid Journal of Chemical Technology & Biotechnology, 82 (11). pp. 964-970. ISSN 0268-2575 T Technology (General) Article PeerReviewed 2007 ftindianacasci 2017-09-15T22:09:53Z Isoniazid is a useful antitubercular drug widely employed in combination therapy with rifampicin. The synthesis of isoniazid from ethyl isonicotinate and hydrazine hydrate was studied in non-aqueous media via lipase-catalyzed hydrazinolysis under both conventional heating and microwave irradiation by using different supported lipases. Among three different commercial lipases used, namely Novozym 435 (Candida antarctica lipase), Lipozyme RM IM (Rhizomucor miehei lipase) and Lipozyme TL IM (Thermomyces lanuginosus lipase), Novozym 435 was found to be the most effective, with conversion of 54% for equimolar concentrations at 50 °C in 4 h. The rate of reaction as well as final conversion increased synergistically under microwave irradiation in comparison with conventional heating, which showed 36.4% conversion, even after 24 h, for the control experiment. Effects of various process parameters such as speed of agitation, catalyst loading, substrate concentration, product concentration and temperature were studied. A kinetic model is also described. Article in Journal/Newspaper Antarc* Antarctica Indian Academy of Sciences: Publication of Fellows |
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Indian Academy of Sciences: Publication of Fellows |
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T Technology (General) |
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T Technology (General) Yadav, Ganapati D. Sajgure, Ashwini D. Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
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T Technology (General) |
description |
Isoniazid is a useful antitubercular drug widely employed in combination therapy with rifampicin. The synthesis of isoniazid from ethyl isonicotinate and hydrazine hydrate was studied in non-aqueous media via lipase-catalyzed hydrazinolysis under both conventional heating and microwave irradiation by using different supported lipases. Among three different commercial lipases used, namely Novozym 435 (Candida antarctica lipase), Lipozyme RM IM (Rhizomucor miehei lipase) and Lipozyme TL IM (Thermomyces lanuginosus lipase), Novozym 435 was found to be the most effective, with conversion of 54% for equimolar concentrations at 50 °C in 4 h. The rate of reaction as well as final conversion increased synergistically under microwave irradiation in comparison with conventional heating, which showed 36.4% conversion, even after 24 h, for the control experiment. Effects of various process parameters such as speed of agitation, catalyst loading, substrate concentration, product concentration and temperature were studied. A kinetic model is also described. |
format |
Article in Journal/Newspaper |
author |
Yadav, Ganapati D. Sajgure, Ashwini D. |
author_facet |
Yadav, Ganapati D. Sajgure, Ashwini D. |
author_sort |
Yadav, Ganapati D. |
title |
Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
title_short |
Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
title_full |
Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
title_fullStr |
Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
title_full_unstemmed |
Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
title_sort |
synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid |
publisher |
John Wiley & Sons, Inc |
publishDate |
2007 |
url |
http://repository.ias.ac.in/111662/ http://onlinelibrary.wiley.com/doi/10.1002/jctb.1738/abstract |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Yadav, Ganapati D. Sajgure, Ashwini D. (2007) Synergism of microwave irradiation and enzyme catalysis in synthesis of isoniazid Journal of Chemical Technology & Biotechnology, 82 (11). pp. 964-970. ISSN 0268-2575 |
_version_ |
1766120186119716864 |