Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta)
Piscine orthoreovirus -1 (PRV-1) causes the disease heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon, and the virus has been detected in wild anadromous Atlantic salmon and brown trout. However, the infection prevalence, viral kinetics, and disease severity in different life s...
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Online Access: | https://hdl.handle.net/11250/3111682 https://doi.org/10.3389/fmars.2023.1151577 |
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ftimr:oai:imr.brage.unit.no:11250/3111682 2024-02-11T10:02:00+01:00 Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) Kannimuthu, Dhamotharan Roh, HyeongJin Morton, Hugh Craig Penaranda, Ma Michelle Demogina Vossgård, Anne Hansen, Tom Johnny Fjelldal, Per Gunnar Karlsbakk, Egil Erlingsson Fiksdal, Ingrid Uglenes Dahle, Maria Berg-Rolness, Hakon Schau Mæhle, Stig Ghebretnsae, Dawit Berhe Nordbø, Joachim Patel, Sonal Madhun, Abdullah Sami Grove, Søren Kvamme, Bjørn Olav 2023 application/pdf https://hdl.handle.net/11250/3111682 https://doi.org/10.3389/fmars.2023.1151577 eng eng Havforskningsinstituttet: 15821 Frontiers in Marine Science. 2023, 10 . urn:issn:2296-7745 https://hdl.handle.net/11250/3111682 https://doi.org/10.3389/fmars.2023.1151577 cristin:2150496 17 10 Frontiers in Marine Science Peer reviewed Journal article 2023 ftimr https://doi.org/10.3389/fmars.2023.1151577 2024-01-17T23:47:42Z Piscine orthoreovirus -1 (PRV-1) causes the disease heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon, and the virus has been detected in wild anadromous Atlantic salmon and brown trout. However, the infection prevalence, viral kinetics, and disease severity in different life stages of Atlantic salmon and brown trout are unknown. The current study aimed to evaluate and compare susceptibility to PRV-1 infection and development of HSMI in different life stages of anadromous Atlantic salmon (Salmo salar) and brown trout (Salmo trutta). We challenged Atlantic salmon and brown trout fry, parr, and post-smolts with PRV-1 by bath, cohabitation, or IP injection. The kinetics of viral infection and disease development were evaluated by RT-qPCR, in situ hybridization, and histology. Our results indicated that PRV-1 infection prevalence and viral kinetics depend on the developmental stage and challenge method in both Atlantic salmon and brown trout. All developmental stages of Atlantic salmon and brown trout can be infected with PRV-1. However, brown trout showed a lower infection prevalence, with positive cases exhibiting only mild infections without any pathological changes in the target organs, while all life stages of Atlantic salmon developed heart lesions characteristic of HSMI. These results strongly suggest that brown trout are less susceptible to PRV-1 infection than Atlantic salmon and further confirm the species-specific susceptibility and disease development for PRV-1 infection. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Frontiers in Marine Science 10 |
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Open Polar |
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Institute for Marine Research: Brage IMR |
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ftimr |
language |
English |
description |
Piscine orthoreovirus -1 (PRV-1) causes the disease heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon, and the virus has been detected in wild anadromous Atlantic salmon and brown trout. However, the infection prevalence, viral kinetics, and disease severity in different life stages of Atlantic salmon and brown trout are unknown. The current study aimed to evaluate and compare susceptibility to PRV-1 infection and development of HSMI in different life stages of anadromous Atlantic salmon (Salmo salar) and brown trout (Salmo trutta). We challenged Atlantic salmon and brown trout fry, parr, and post-smolts with PRV-1 by bath, cohabitation, or IP injection. The kinetics of viral infection and disease development were evaluated by RT-qPCR, in situ hybridization, and histology. Our results indicated that PRV-1 infection prevalence and viral kinetics depend on the developmental stage and challenge method in both Atlantic salmon and brown trout. All developmental stages of Atlantic salmon and brown trout can be infected with PRV-1. However, brown trout showed a lower infection prevalence, with positive cases exhibiting only mild infections without any pathological changes in the target organs, while all life stages of Atlantic salmon developed heart lesions characteristic of HSMI. These results strongly suggest that brown trout are less susceptible to PRV-1 infection than Atlantic salmon and further confirm the species-specific susceptibility and disease development for PRV-1 infection. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Kannimuthu, Dhamotharan Roh, HyeongJin Morton, Hugh Craig Penaranda, Ma Michelle Demogina Vossgård, Anne Hansen, Tom Johnny Fjelldal, Per Gunnar Karlsbakk, Egil Erlingsson Fiksdal, Ingrid Uglenes Dahle, Maria Berg-Rolness, Hakon Schau Mæhle, Stig Ghebretnsae, Dawit Berhe Nordbø, Joachim Patel, Sonal Madhun, Abdullah Sami Grove, Søren Kvamme, Bjørn Olav |
spellingShingle |
Kannimuthu, Dhamotharan Roh, HyeongJin Morton, Hugh Craig Penaranda, Ma Michelle Demogina Vossgård, Anne Hansen, Tom Johnny Fjelldal, Per Gunnar Karlsbakk, Egil Erlingsson Fiksdal, Ingrid Uglenes Dahle, Maria Berg-Rolness, Hakon Schau Mæhle, Stig Ghebretnsae, Dawit Berhe Nordbø, Joachim Patel, Sonal Madhun, Abdullah Sami Grove, Søren Kvamme, Bjørn Olav Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) |
author_facet |
Kannimuthu, Dhamotharan Roh, HyeongJin Morton, Hugh Craig Penaranda, Ma Michelle Demogina Vossgård, Anne Hansen, Tom Johnny Fjelldal, Per Gunnar Karlsbakk, Egil Erlingsson Fiksdal, Ingrid Uglenes Dahle, Maria Berg-Rolness, Hakon Schau Mæhle, Stig Ghebretnsae, Dawit Berhe Nordbø, Joachim Patel, Sonal Madhun, Abdullah Sami Grove, Søren Kvamme, Bjørn Olav |
author_sort |
Kannimuthu, Dhamotharan |
title |
Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) |
title_short |
Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) |
title_full |
Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) |
title_fullStr |
Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) |
title_full_unstemmed |
Experimental transmission of piscine orthoreovirus-1 (PRV-1) in different life stages of Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) |
title_sort |
experimental transmission of piscine orthoreovirus-1 (prv-1) in different life stages of atlantic salmon (salmo salar) and brown trout (salmo trutta) |
publishDate |
2023 |
url |
https://hdl.handle.net/11250/3111682 https://doi.org/10.3389/fmars.2023.1151577 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
17 10 Frontiers in Marine Science |
op_relation |
Havforskningsinstituttet: 15821 Frontiers in Marine Science. 2023, 10 . urn:issn:2296-7745 https://hdl.handle.net/11250/3111682 https://doi.org/10.3389/fmars.2023.1151577 cristin:2150496 |
op_doi |
https://doi.org/10.3389/fmars.2023.1151577 |
container_title |
Frontiers in Marine Science |
container_volume |
10 |
_version_ |
1790597901574995968 |