Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model
Acid mine drainage (AMD) is widely acknowledged as a substantial threat to the biodiversity of aquatic ecosystems. The present study aimed to study the toxicological effects of Cu-rich AMD from the Sulitjelma mine in zebrafish larvae. The AMD from this mine was found to contain elevated levels of di...
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ftimr:oai:imr.brage.unit.no:11250/3109799 2024-02-04T10:04:53+01:00 Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model Varshney, Shubham Lundås, Mikkel Siriyappagouder, Prabhugouda Kristensen, Torstein Olsvik, Pål Asgeir 2024 application/pdf https://hdl.handle.net/11250/3109799 https://doi.org/10.1016/j.ecoenv.2023.115796 eng eng urn:issn:0147-6513 https://hdl.handle.net/11250/3109799 https://doi.org/10.1016/j.ecoenv.2023.115796 cristin:2211574 Ecotoxicology and Environmental Safety Others 2024 ftimr https://doi.org/10.1016/j.ecoenv.2023.115796 2024-01-10T23:47:43Z Acid mine drainage (AMD) is widely acknowledged as a substantial threat to the biodiversity of aquatic ecosystems. The present study aimed to study the toxicological effects of Cu-rich AMD from the Sulitjelma mine in zebrafish larvae. The AMD from this mine was found to contain elevated levels of dissolved metals including Mg (46.7 mg/L), Al (20.2 mg/L), Cu (18.3 mg/L), Fe (19.8 mg/L) and Zn (10.6 mg/L). To investigate the toxicological effects, the study commenced by exposing zebrafish embryos to various concentrations of AMD (ranging from 0.75% to 9%) to determine the median lethal concentration (LC50). Results showed that 96 h LC50 for zebrafish larvae following AMD exposure was 2.86% (95% CI: 2.32–3.52%). Based on acute toxicity results, zebrafish embryos (<2 hpf) were exposed to 0.1% AMD (Cu: 21.7 µg/L) and 0.45% AMD (Cu: 85.7 µg/L) for 96 h to assess development, swimming behaviour, heart rate, respiration and transcriptional responses at 116 hpf. Light microscopy results showed that both 0.1% and 0.45% AMD reduced the body length, eye size and swim bladder area of zebrafish larvae and caused phenotypic abnormalities. Swimming behaviour results showed that 0.45% AMD significantly decreased the locomotion of zebrafish larvae. Heart rate was not affected by AMD exposure. Furthermore, exposure caused a significant increase in oxygen consumption indicating vascular stress in developing larvae. Taken altogether, the study shows that even heavily diluted AMD with environmentally relevant levels of Cu caused toxicity in zebrafish larvae. acceptedVersion Other/Unknown Material Sulitjelma Institute for Marine Research: Brage IMR Sulitjelma ENVELOPE(16.077,16.077,67.133,67.133) Ecotoxicology and Environmental Safety 269 115796 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
Acid mine drainage (AMD) is widely acknowledged as a substantial threat to the biodiversity of aquatic ecosystems. The present study aimed to study the toxicological effects of Cu-rich AMD from the Sulitjelma mine in zebrafish larvae. The AMD from this mine was found to contain elevated levels of dissolved metals including Mg (46.7 mg/L), Al (20.2 mg/L), Cu (18.3 mg/L), Fe (19.8 mg/L) and Zn (10.6 mg/L). To investigate the toxicological effects, the study commenced by exposing zebrafish embryos to various concentrations of AMD (ranging from 0.75% to 9%) to determine the median lethal concentration (LC50). Results showed that 96 h LC50 for zebrafish larvae following AMD exposure was 2.86% (95% CI: 2.32–3.52%). Based on acute toxicity results, zebrafish embryos (<2 hpf) were exposed to 0.1% AMD (Cu: 21.7 µg/L) and 0.45% AMD (Cu: 85.7 µg/L) for 96 h to assess development, swimming behaviour, heart rate, respiration and transcriptional responses at 116 hpf. Light microscopy results showed that both 0.1% and 0.45% AMD reduced the body length, eye size and swim bladder area of zebrafish larvae and caused phenotypic abnormalities. Swimming behaviour results showed that 0.45% AMD significantly decreased the locomotion of zebrafish larvae. Heart rate was not affected by AMD exposure. Furthermore, exposure caused a significant increase in oxygen consumption indicating vascular stress in developing larvae. Taken altogether, the study shows that even heavily diluted AMD with environmentally relevant levels of Cu caused toxicity in zebrafish larvae. acceptedVersion |
format |
Other/Unknown Material |
author |
Varshney, Shubham Lundås, Mikkel Siriyappagouder, Prabhugouda Kristensen, Torstein Olsvik, Pål Asgeir |
spellingShingle |
Varshney, Shubham Lundås, Mikkel Siriyappagouder, Prabhugouda Kristensen, Torstein Olsvik, Pål Asgeir Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model |
author_facet |
Varshney, Shubham Lundås, Mikkel Siriyappagouder, Prabhugouda Kristensen, Torstein Olsvik, Pål Asgeir |
author_sort |
Varshney, Shubham |
title |
Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model |
title_short |
Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model |
title_full |
Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model |
title_fullStr |
Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model |
title_full_unstemmed |
Ecotoxicological assessment of Cu-rich acid mine drainage of Sulitjelma mine using zebrafish larvae as an animal model |
title_sort |
ecotoxicological assessment of cu-rich acid mine drainage of sulitjelma mine using zebrafish larvae as an animal model |
publishDate |
2024 |
url |
https://hdl.handle.net/11250/3109799 https://doi.org/10.1016/j.ecoenv.2023.115796 |
long_lat |
ENVELOPE(16.077,16.077,67.133,67.133) |
geographic |
Sulitjelma |
geographic_facet |
Sulitjelma |
genre |
Sulitjelma |
genre_facet |
Sulitjelma |
op_source |
Ecotoxicology and Environmental Safety |
op_relation |
urn:issn:0147-6513 https://hdl.handle.net/11250/3109799 https://doi.org/10.1016/j.ecoenv.2023.115796 cristin:2211574 |
op_doi |
https://doi.org/10.1016/j.ecoenv.2023.115796 |
container_title |
Ecotoxicology and Environmental Safety |
container_volume |
269 |
container_start_page |
115796 |
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1789973637446500352 |