Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition
Using germ cell-free (GCF), sterile, dnd-knockout salmon for farming could solve the problems associated with precocious maturation and genetic introgression of farmed breeds into wild populations. However, prior to using GCF fish in the salmon farming industry, it is crucial to understand if, or ho...
Published in: | Aquaculture |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/3048809 https://doi.org/10.1016/j.aquaculture.2022.738456 |
id |
ftimr:oai:imr.brage.unit.no:11250/3048809 |
---|---|
record_format |
openpolar |
spelling |
ftimr:oai:imr.brage.unit.no:11250/3048809 2023-05-15T15:32:46+02:00 Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition Kleppe, Lene Fjelldal, Per Gunnar Andersson, Eva Hansen, Tom Johnny Sanden, Monica Bruvik, Aina Skaftnesmo, Kai Ove Furmanek, Tomasz Kjærner-Semb, Erik Nordtorp Crespo, Diego Flavell, Simon Pedersen, Audun Østby Vogelsang, Petra Torsvik, Anja Kvestad, Karen Anita Olausson, Sara Norberg, Birgitta Schulz, Rüdiger W. Bogerd, J. Santi, N. Edvardsen, Rolf Brudvik Wargelius, Anna 2022 application/pdf https://hdl.handle.net/11250/3048809 https://doi.org/10.1016/j.aquaculture.2022.738456 eng eng Aquaculture. 2022, 560 . urn:issn:0044-8486 https://hdl.handle.net/11250/3048809 https://doi.org/10.1016/j.aquaculture.2022.738456 cristin:2052973 0 560 Aquaculture Peer reviewed Journal article 2022 ftimr https://doi.org/10.1016/j.aquaculture.2022.738456 2023-02-13T09:34:21Z Using germ cell-free (GCF), sterile, dnd-knockout salmon for farming could solve the problems associated with precocious maturation and genetic introgression of farmed breeds into wild populations. However, prior to using GCF fish in the salmon farming industry, it is crucial to understand if, or how, the GCF phenotype differs from wild type (WT) counterparts in terms of growth and welfare. To characterize the GCF phenotype throughout a production cycle, we reared GCF and WT salmon in indoor common garden tanks for 3 years, until harvest size. Regarding body size, smoltification markers (mRNA levels of gill Na+/K+-ATPase [NKA] subunits), plasma stress indicators (pH, glucose, sodium, chloride, calcium), relative heart size, prevalence of vertebra deformities and fillet proximate composition, GCF fish could not be distinguished from WTs. Transient differences were detected in plasma concentrations of lactate and osmolality, and only a few genes were differentially expressed in WT and GCF transcriptomes of muscle and pituitary. At harvest, fillets from GCF and WT salmon contained the same amount of omega-3 fatty acids, however the relative content of omega-3 fatty acids was higher in GCF compared to WT males. Towards harvest size, body growth rate, condition factor and relative liver size were significantly higher in WT than in GCF fish, probably relating to initiation of puberty in WTs. Since GCF salmon never become sexually mature, it is possible to postpone the time of harvest to exploit the growth potential uninhibited by sexual maturation. In conclusion, GCF salmon performed to a large extent similarly to their WT counterparts but had the clear advantage of never maturing. publishedVersion Article in Journal/Newspaper Atlantic salmon Institute for Marine Research: Brage IMR Aquaculture 560 738456 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
Using germ cell-free (GCF), sterile, dnd-knockout salmon for farming could solve the problems associated with precocious maturation and genetic introgression of farmed breeds into wild populations. However, prior to using GCF fish in the salmon farming industry, it is crucial to understand if, or how, the GCF phenotype differs from wild type (WT) counterparts in terms of growth and welfare. To characterize the GCF phenotype throughout a production cycle, we reared GCF and WT salmon in indoor common garden tanks for 3 years, until harvest size. Regarding body size, smoltification markers (mRNA levels of gill Na+/K+-ATPase [NKA] subunits), plasma stress indicators (pH, glucose, sodium, chloride, calcium), relative heart size, prevalence of vertebra deformities and fillet proximate composition, GCF fish could not be distinguished from WTs. Transient differences were detected in plasma concentrations of lactate and osmolality, and only a few genes were differentially expressed in WT and GCF transcriptomes of muscle and pituitary. At harvest, fillets from GCF and WT salmon contained the same amount of omega-3 fatty acids, however the relative content of omega-3 fatty acids was higher in GCF compared to WT males. Towards harvest size, body growth rate, condition factor and relative liver size were significantly higher in WT than in GCF fish, probably relating to initiation of puberty in WTs. Since GCF salmon never become sexually mature, it is possible to postpone the time of harvest to exploit the growth potential uninhibited by sexual maturation. In conclusion, GCF salmon performed to a large extent similarly to their WT counterparts but had the clear advantage of never maturing. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Kleppe, Lene Fjelldal, Per Gunnar Andersson, Eva Hansen, Tom Johnny Sanden, Monica Bruvik, Aina Skaftnesmo, Kai Ove Furmanek, Tomasz Kjærner-Semb, Erik Nordtorp Crespo, Diego Flavell, Simon Pedersen, Audun Østby Vogelsang, Petra Torsvik, Anja Kvestad, Karen Anita Olausson, Sara Norberg, Birgitta Schulz, Rüdiger W. Bogerd, J. Santi, N. Edvardsen, Rolf Brudvik Wargelius, Anna |
spellingShingle |
Kleppe, Lene Fjelldal, Per Gunnar Andersson, Eva Hansen, Tom Johnny Sanden, Monica Bruvik, Aina Skaftnesmo, Kai Ove Furmanek, Tomasz Kjærner-Semb, Erik Nordtorp Crespo, Diego Flavell, Simon Pedersen, Audun Østby Vogelsang, Petra Torsvik, Anja Kvestad, Karen Anita Olausson, Sara Norberg, Birgitta Schulz, Rüdiger W. Bogerd, J. Santi, N. Edvardsen, Rolf Brudvik Wargelius, Anna Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
author_facet |
Kleppe, Lene Fjelldal, Per Gunnar Andersson, Eva Hansen, Tom Johnny Sanden, Monica Bruvik, Aina Skaftnesmo, Kai Ove Furmanek, Tomasz Kjærner-Semb, Erik Nordtorp Crespo, Diego Flavell, Simon Pedersen, Audun Østby Vogelsang, Petra Torsvik, Anja Kvestad, Karen Anita Olausson, Sara Norberg, Birgitta Schulz, Rüdiger W. Bogerd, J. Santi, N. Edvardsen, Rolf Brudvik Wargelius, Anna |
author_sort |
Kleppe, Lene |
title |
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
title_short |
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
title_full |
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
title_fullStr |
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
title_full_unstemmed |
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
title_sort |
full production cycle performance of gene-edited, sterile atlantic salmon - growth, smoltification, welfare indicators and fillet composition |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3048809 https://doi.org/10.1016/j.aquaculture.2022.738456 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
0 560 Aquaculture |
op_relation |
Aquaculture. 2022, 560 . urn:issn:0044-8486 https://hdl.handle.net/11250/3048809 https://doi.org/10.1016/j.aquaculture.2022.738456 cristin:2052973 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2022.738456 |
container_title |
Aquaculture |
container_volume |
560 |
container_start_page |
738456 |
_version_ |
1766363259657519104 |