Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds
Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Se...
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Online Access: | https://hdl.handle.net/11250/3042653 https://doi.org/10.3389/fmicb.2022.1005625 |
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ftimr:oai:imr.brage.unit.no:11250/3042653 2023-05-15T14:55:06+02:00 Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds Schneider, Yannik Karl Heinz Hagestad, Ole Christian Li, Chun Hansen, Espen Holst Andersen, Jeanette Hammer 2022 application/pdf https://hdl.handle.net/11250/3042653 https://doi.org/10.3389/fmicb.2022.1005625 eng eng Frontiers in Microbiology. 2022, 13 . urn:issn:1664-302X https://hdl.handle.net/11250/3042653 https://doi.org/10.3389/fmicb.2022.1005625 cristin:2066160 16 13 Frontiers in Microbiology Peer reviewed Journal article 2022 ftimr https://doi.org/10.3389/fmicb.2022.1005625 2023-01-18T23:43:45Z Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Sea. In the frame of the isolation campaign, it was investigated how different sample treatments, isolation media and sample-sources, such as animals and sediments, affected the yield of actinobacterial isolates to aid further isolation campaigns. Special attention was given to sediments, where we expected spores of spore forming bacteria to enrich. Beside actinobacteria a high share of bacilli was obtained which was not desired. An experimental protocol for down-scaled cultivation and extraction was tested and compared with an established low-throughput cultivation and extraction protocol. The heat-shock method proved suitable to enrich spore-, or endospore forming bacteria such as bacilli. Finally, a group bioactive compounds could be tentatively identified using UHPLC–MS/MS analysis of the active fractions. publishedVersion Article in Journal/Newspaper Arctic Barents Sea Greenland Greenland Sea Norwegian Sea Institute for Marine Research: Brage IMR Arctic Barents Sea Norwegian Sea Greenland Frontiers in Microbiology 13 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
Actinobacteria are among the most prolific producers of bioactive secondary metabolites. In order to collect Arctic marine bacteria for the discovery of new bioactive metabolites, actinobacteria were selectively isolated during a research cruise in the Greenland Sea, Norwegian Sea and the Barents Sea. In the frame of the isolation campaign, it was investigated how different sample treatments, isolation media and sample-sources, such as animals and sediments, affected the yield of actinobacterial isolates to aid further isolation campaigns. Special attention was given to sediments, where we expected spores of spore forming bacteria to enrich. Beside actinobacteria a high share of bacilli was obtained which was not desired. An experimental protocol for down-scaled cultivation and extraction was tested and compared with an established low-throughput cultivation and extraction protocol. The heat-shock method proved suitable to enrich spore-, or endospore forming bacteria such as bacilli. Finally, a group bioactive compounds could be tentatively identified using UHPLC–MS/MS analysis of the active fractions. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Schneider, Yannik Karl Heinz Hagestad, Ole Christian Li, Chun Hansen, Espen Holst Andersen, Jeanette Hammer |
spellingShingle |
Schneider, Yannik Karl Heinz Hagestad, Ole Christian Li, Chun Hansen, Espen Holst Andersen, Jeanette Hammer Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
author_facet |
Schneider, Yannik Karl Heinz Hagestad, Ole Christian Li, Chun Hansen, Espen Holst Andersen, Jeanette Hammer |
author_sort |
Schneider, Yannik Karl Heinz |
title |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_short |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_full |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_fullStr |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_full_unstemmed |
Selective isolation of Arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
title_sort |
selective isolation of arctic marine actinobacteria and a down-scaled fermentation and extraction strategy for identifying bioactive compounds |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3042653 https://doi.org/10.3389/fmicb.2022.1005625 |
geographic |
Arctic Barents Sea Norwegian Sea Greenland |
geographic_facet |
Arctic Barents Sea Norwegian Sea Greenland |
genre |
Arctic Barents Sea Greenland Greenland Sea Norwegian Sea |
genre_facet |
Arctic Barents Sea Greenland Greenland Sea Norwegian Sea |
op_source |
16 13 Frontiers in Microbiology |
op_relation |
Frontiers in Microbiology. 2022, 13 . urn:issn:1664-302X https://hdl.handle.net/11250/3042653 https://doi.org/10.3389/fmicb.2022.1005625 cristin:2066160 |
op_doi |
https://doi.org/10.3389/fmicb.2022.1005625 |
container_title |
Frontiers in Microbiology |
container_volume |
13 |
_version_ |
1766326885631918080 |