Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua)
Inclusion of new environmental toxicants increase with the amount of plant ingredients substituting marine proteins and oils in feed for farmed Atlantic salmon (Salma salar). Agricultural pesticides like chlorpyrifos-methyl, present in commercial salmon feeds, may affect salmon immune and detoxifica...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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Online Access: | https://hdl.handle.net/11250/3027016 https://doi.org/10.1016/j.fsi.2022.06.060 |
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ftimr:oai:imr.brage.unit.no:11250/3027016 2023-05-15T15:27:16+02:00 Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) Holen, Elisabeth Espe, Marit Larsen, Anett Kristin Olsvik, Pål Asgeir 2022 application/pdf https://hdl.handle.net/11250/3027016 https://doi.org/10.1016/j.fsi.2022.06.060 eng eng Regionale forskningsfond Nord-Norge: 298990 Fish and Shellfish Immunology. 2022, 127 549-560. urn:issn:1050-4648 https://hdl.handle.net/11250/3027016 https://doi.org/10.1016/j.fsi.2022.06.060 cristin:2058316 549-560 127 Fish and Shellfish Immunology Peer reviewed Journal article 2022 ftimr https://doi.org/10.1016/j.fsi.2022.06.060 2022-10-26T22:42:26Z Inclusion of new environmental toxicants increase with the amount of plant ingredients substituting marine proteins and oils in feed for farmed Atlantic salmon (Salma salar). Agricultural pesticides like chlorpyrifos-methyl, present in commercial salmon feeds, may affect salmon immune and detoxification responses. Atlantic cod (Gadus morhua), surrounding the net pens, grazing on feces and uneaten pellets may be affected accordingly. The aim of this study was to analyze transcription responses in Atlantic cod head kidney tissue and isolated leukocytes following dietary chlorpyrifos-methyl inclusions and possible interactions with proinflammatory signals. Head kidney tissues and leukocytes were isolated from cod fed diets contaminated with chlorpyrifos-methyl (0.5 mg/kg, 2.4 mg/kg, 23.2 mg/kg) for 30 days. The isolated leukocytes were further challenged with bacteria (lipopolysaccharide (LPS), virus (polyinosinic acid:polycytidylic acid (PIC) mimic and l-arginine, an immuno-modulating amino acid, in vitro. The LPS-induced transcription of the interleukin genes il-1β, il-6, il-8 increased in leukocytes isolated from cod fed chlorpyrifos-methyl 23.2 mg/kg, compared to cod fed the control diet, indicating increased inflammation. Transcriptional levels of carnitine palmitoyl transferase (cpt1a), aryl hydrogen receptor (ahr) and catalase (cat) were all reduced by dietary inclusions of chlorpyrifos-methyl in the leukocytes. The findings suggests that dietary chlorpyrifos-methyl exposure impair inflammation, detoxification and redox signaling in cod leukocytes. publishedVersion Article in Journal/Newspaper atlantic cod Atlantic salmon Gadus morhua Institute for Marine Research: Brage IMR Salma ENVELOPE(32.133,32.133,65.817,65.817) Fish & Shellfish Immunology 127 549 560 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
Inclusion of new environmental toxicants increase with the amount of plant ingredients substituting marine proteins and oils in feed for farmed Atlantic salmon (Salma salar). Agricultural pesticides like chlorpyrifos-methyl, present in commercial salmon feeds, may affect salmon immune and detoxification responses. Atlantic cod (Gadus morhua), surrounding the net pens, grazing on feces and uneaten pellets may be affected accordingly. The aim of this study was to analyze transcription responses in Atlantic cod head kidney tissue and isolated leukocytes following dietary chlorpyrifos-methyl inclusions and possible interactions with proinflammatory signals. Head kidney tissues and leukocytes were isolated from cod fed diets contaminated with chlorpyrifos-methyl (0.5 mg/kg, 2.4 mg/kg, 23.2 mg/kg) for 30 days. The isolated leukocytes were further challenged with bacteria (lipopolysaccharide (LPS), virus (polyinosinic acid:polycytidylic acid (PIC) mimic and l-arginine, an immuno-modulating amino acid, in vitro. The LPS-induced transcription of the interleukin genes il-1β, il-6, il-8 increased in leukocytes isolated from cod fed chlorpyrifos-methyl 23.2 mg/kg, compared to cod fed the control diet, indicating increased inflammation. Transcriptional levels of carnitine palmitoyl transferase (cpt1a), aryl hydrogen receptor (ahr) and catalase (cat) were all reduced by dietary inclusions of chlorpyrifos-methyl in the leukocytes. The findings suggests that dietary chlorpyrifos-methyl exposure impair inflammation, detoxification and redox signaling in cod leukocytes. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Holen, Elisabeth Espe, Marit Larsen, Anett Kristin Olsvik, Pål Asgeir |
spellingShingle |
Holen, Elisabeth Espe, Marit Larsen, Anett Kristin Olsvik, Pål Asgeir Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) |
author_facet |
Holen, Elisabeth Espe, Marit Larsen, Anett Kristin Olsvik, Pål Asgeir |
author_sort |
Holen, Elisabeth |
title |
Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) |
title_short |
Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) |
title_full |
Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) |
title_fullStr |
Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) |
title_full_unstemmed |
Dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (Gadus morhua) |
title_sort |
dietary chlorpyrifos-methyl exposure impair transcription of immune-, detoxification- and redox signaling genes in leukocytes isolated from cod (gadus morhua) |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3027016 https://doi.org/10.1016/j.fsi.2022.06.060 |
long_lat |
ENVELOPE(32.133,32.133,65.817,65.817) |
geographic |
Salma |
geographic_facet |
Salma |
genre |
atlantic cod Atlantic salmon Gadus morhua |
genre_facet |
atlantic cod Atlantic salmon Gadus morhua |
op_source |
549-560 127 Fish and Shellfish Immunology |
op_relation |
Regionale forskningsfond Nord-Norge: 298990 Fish and Shellfish Immunology. 2022, 127 549-560. urn:issn:1050-4648 https://hdl.handle.net/11250/3027016 https://doi.org/10.1016/j.fsi.2022.06.060 cristin:2058316 |
op_doi |
https://doi.org/10.1016/j.fsi.2022.06.060 |
container_title |
Fish & Shellfish Immunology |
container_volume |
127 |
container_start_page |
549 |
op_container_end_page |
560 |
_version_ |
1766357718947332096 |