In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model
A novel 3D Atlantic salmon co-culture model was developed using primary hepatocytes and kidney epithelial cells isolated from the same fish. Mono and co-cultures of primary hepatocytes and kidney epithelial cells were exposed for 48 h to glyphosate (5, 50 and 500 μM). For comparison, cells were also...
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Online Access: | https://hdl.handle.net/11250/3017124 https://doi.org/10.1016/j.fct.2022.113012 |
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ftimr:oai:imr.brage.unit.no:11250/3017124 2023-05-15T15:30:45+02:00 In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model Søfteland, Liv Ingeborg Rosvoll Olsvik, Pål Asgeir 2022 application/pdf https://hdl.handle.net/11250/3017124 https://doi.org/10.1016/j.fct.2022.113012 eng eng Food and Chemical Toxicology. 2022, 164 . urn:issn:0278-6915 https://hdl.handle.net/11250/3017124 https://doi.org/10.1016/j.fct.2022.113012 cristin:2047670 0 164 Food and Chemical Toxicology Peer reviewed Journal article 2022 ftimr https://doi.org/10.1016/j.fct.2022.113012 2022-09-14T22:41:47Z A novel 3D Atlantic salmon co-culture model was developed using primary hepatocytes and kidney epithelial cells isolated from the same fish. Mono and co-cultures of primary hepatocytes and kidney epithelial cells were exposed for 48 h to glyphosate (5, 50 and 500 μM). For comparison, cells were also exposed to chlorpyrifos, benzo(a)pyrene and cadmium. Cell staining, cell viability assessments, RT-qPCR and global metabolomic profiling were used to examine the toxicological effects on liver and renal function and to compare responses in 3D and 2D cultures. The 3D hepatocyte cell culture was considered superior to the 2D culture due to the ATP binding cassette subfamily B member 1 (Abcb1) response and was thus used further in co-culture with kidney cells. Metabolomic analysis of co-cultured cells showed that glyphosate exposure (500 μM) altered lipid metabolism in both hepatocytes and kidney cells. Elevated levels of several types of PUFAs and long-chain fatty acids were observed in exposed hepatocytes, owing to increased uptake and phospholipid remodelling. Glyphosate suppressed the expression of estrogen receptor 1 (Esr1) and vitellogenin (Vtg) and altered histidine metabolism in exposed hepatocytes. Increased levels of cholesterol and downregulation of clusterin (Clu) suggest that glyphosate treatment affected membrane stability in Atlantic salmon kidney cells. This study demonstrates the usefulness of applying 3D co-culture models in risk assessment. publishedVersion Article in Journal/Newspaper Atlantic salmon Institute for Marine Research: Brage IMR Food and Chemical Toxicology 164 113012 |
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Institute for Marine Research: Brage IMR |
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English |
description |
A novel 3D Atlantic salmon co-culture model was developed using primary hepatocytes and kidney epithelial cells isolated from the same fish. Mono and co-cultures of primary hepatocytes and kidney epithelial cells were exposed for 48 h to glyphosate (5, 50 and 500 μM). For comparison, cells were also exposed to chlorpyrifos, benzo(a)pyrene and cadmium. Cell staining, cell viability assessments, RT-qPCR and global metabolomic profiling were used to examine the toxicological effects on liver and renal function and to compare responses in 3D and 2D cultures. The 3D hepatocyte cell culture was considered superior to the 2D culture due to the ATP binding cassette subfamily B member 1 (Abcb1) response and was thus used further in co-culture with kidney cells. Metabolomic analysis of co-cultured cells showed that glyphosate exposure (500 μM) altered lipid metabolism in both hepatocytes and kidney cells. Elevated levels of several types of PUFAs and long-chain fatty acids were observed in exposed hepatocytes, owing to increased uptake and phospholipid remodelling. Glyphosate suppressed the expression of estrogen receptor 1 (Esr1) and vitellogenin (Vtg) and altered histidine metabolism in exposed hepatocytes. Increased levels of cholesterol and downregulation of clusterin (Clu) suggest that glyphosate treatment affected membrane stability in Atlantic salmon kidney cells. This study demonstrates the usefulness of applying 3D co-culture models in risk assessment. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Søfteland, Liv Ingeborg Rosvoll Olsvik, Pål Asgeir |
spellingShingle |
Søfteland, Liv Ingeborg Rosvoll Olsvik, Pål Asgeir In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model |
author_facet |
Søfteland, Liv Ingeborg Rosvoll Olsvik, Pål Asgeir |
author_sort |
Søfteland, Liv Ingeborg Rosvoll |
title |
In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model |
title_short |
In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model |
title_full |
In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model |
title_fullStr |
In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model |
title_full_unstemmed |
In vitro toxicity of glyphosate in Atlantic salmon evaluated with a 3D hepatocyte-kidney co-culture model |
title_sort |
in vitro toxicity of glyphosate in atlantic salmon evaluated with a 3d hepatocyte-kidney co-culture model |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3017124 https://doi.org/10.1016/j.fct.2022.113012 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
0 164 Food and Chemical Toxicology |
op_relation |
Food and Chemical Toxicology. 2022, 164 . urn:issn:0278-6915 https://hdl.handle.net/11250/3017124 https://doi.org/10.1016/j.fct.2022.113012 cristin:2047670 |
op_doi |
https://doi.org/10.1016/j.fct.2022.113012 |
container_title |
Food and Chemical Toxicology |
container_volume |
164 |
container_start_page |
113012 |
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1766361207714873344 |