Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets
It is well established that farmed Atlantic salmon (Salmo salar) need n3-highly unsaturated fatty acids (HUFA) in their diet to thrive and grow. However, the biological functions to the individual HUFAs may differ, implying that future supplementation could require fixed ratios for maximum benefit....
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Online Access: | https://hdl.handle.net/11250/3015763 https://doi.org/10.1016/j.aquaculture.2022.738318 |
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ftimr:oai:imr.brage.unit.no:11250/3015763 2023-05-15T15:31:27+02:00 Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets Løvmo, Signe Dille Sundh, Henrik Whatmore, Paul Nordvi, Malene Fosse Sigholt, Trygve Madaro, Angelico Bardal, Tora Olsen, Rolf Erik 2022 application/pdf https://hdl.handle.net/11250/3015763 https://doi.org/10.1016/j.aquaculture.2022.738318 eng eng Aquaculture. 2022, 557 . urn:issn:0044-8486 https://hdl.handle.net/11250/3015763 https://doi.org/10.1016/j.aquaculture.2022.738318 cristin:2046953 Navngivelse-DelPåSammeVilkår 4.0 Internasjonal http://creativecommons.org/licenses/by-sa/4.0/deed.no CC-BY-SA 13 557 Aquaculture 738318 Peer reviewed Journal article 2022 ftimr https://doi.org/10.1016/j.aquaculture.2022.738318 2022-09-07T22:41:47Z It is well established that farmed Atlantic salmon (Salmo salar) need n3-highly unsaturated fatty acids (HUFA) in their diet to thrive and grow. However, the biological functions to the individual HUFAs may differ, implying that future supplementation could require fixed ratios for maximum benefit. The intestinal barrier is essential to f ish health, and any disruption of the barrier can have detrimental effects. The current experiment was designed to examine the response of the intestinal hindgut when fed a low HUFA diet with 8.5 g/kg EPA + DHA (4.5% total fatty acid) or two high HUFA diets, with either high DHA (28.7 g/kg and 5.9% total fatty acid) or high EPA (25 g/kg and 14.2% total fatty acid). The diets were fed to Atlantic salmon post-smolt over 10 weeks and thereafter exposed to 3 weeks of chronic stress. After 10 weeks of feeding there were no differences in intestinal permeability and integrity, but intestinal morphology indicated increased intestinal health in the high EPA group. Gene expression also suggest that fish fed the high EPA diet had more regulation of pathways related to protein turnover compared to the high DHA fed fish. There was also indication of lower energy utilization in the low HUFA fed fish than high HUFA. Subjecting fish to 3 weeks of chronic stress led to a reduction in transepithelial resistance, increased ion flux and active L-lysine transport across the intestinal barrier in addition to a decrease in mucosal fold, enterocyte height and supranuclear vacuole density and an increase in thickness of the intestinal muscularis. After stress, the low HUFA group showed signs of inflammation with increased infiltration of MHCII positive cells. Gene expression also showed that low HUFA fed fish had a lower response to chronic stress compared to the high HUFA groups. Comparing fish fed either high DHA or EPA exposed to chronic stress showed few physical effects, but a lower density of supranuclear vacuoles and upregulation in immune-related gene expression indicate inflammation in the ... Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Aquaculture 557 738318 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
It is well established that farmed Atlantic salmon (Salmo salar) need n3-highly unsaturated fatty acids (HUFA) in their diet to thrive and grow. However, the biological functions to the individual HUFAs may differ, implying that future supplementation could require fixed ratios for maximum benefit. The intestinal barrier is essential to f ish health, and any disruption of the barrier can have detrimental effects. The current experiment was designed to examine the response of the intestinal hindgut when fed a low HUFA diet with 8.5 g/kg EPA + DHA (4.5% total fatty acid) or two high HUFA diets, with either high DHA (28.7 g/kg and 5.9% total fatty acid) or high EPA (25 g/kg and 14.2% total fatty acid). The diets were fed to Atlantic salmon post-smolt over 10 weeks and thereafter exposed to 3 weeks of chronic stress. After 10 weeks of feeding there were no differences in intestinal permeability and integrity, but intestinal morphology indicated increased intestinal health in the high EPA group. Gene expression also suggest that fish fed the high EPA diet had more regulation of pathways related to protein turnover compared to the high DHA fed fish. There was also indication of lower energy utilization in the low HUFA fed fish than high HUFA. Subjecting fish to 3 weeks of chronic stress led to a reduction in transepithelial resistance, increased ion flux and active L-lysine transport across the intestinal barrier in addition to a decrease in mucosal fold, enterocyte height and supranuclear vacuole density and an increase in thickness of the intestinal muscularis. After stress, the low HUFA group showed signs of inflammation with increased infiltration of MHCII positive cells. Gene expression also showed that low HUFA fed fish had a lower response to chronic stress compared to the high HUFA groups. Comparing fish fed either high DHA or EPA exposed to chronic stress showed few physical effects, but a lower density of supranuclear vacuoles and upregulation in immune-related gene expression indicate inflammation in the ... |
format |
Article in Journal/Newspaper |
author |
Løvmo, Signe Dille Sundh, Henrik Whatmore, Paul Nordvi, Malene Fosse Sigholt, Trygve Madaro, Angelico Bardal, Tora Olsen, Rolf Erik |
spellingShingle |
Løvmo, Signe Dille Sundh, Henrik Whatmore, Paul Nordvi, Malene Fosse Sigholt, Trygve Madaro, Angelico Bardal, Tora Olsen, Rolf Erik Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets |
author_facet |
Løvmo, Signe Dille Sundh, Henrik Whatmore, Paul Nordvi, Malene Fosse Sigholt, Trygve Madaro, Angelico Bardal, Tora Olsen, Rolf Erik |
author_sort |
Løvmo, Signe Dille |
title |
Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets |
title_short |
Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets |
title_full |
Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets |
title_fullStr |
Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets |
title_full_unstemmed |
Intestinal health in Atlantic salmon post-smolt (Salmo salar) when fed low- and high HUFA diets |
title_sort |
intestinal health in atlantic salmon post-smolt (salmo salar) when fed low- and high hufa diets |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3015763 https://doi.org/10.1016/j.aquaculture.2022.738318 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
13 557 Aquaculture 738318 |
op_relation |
Aquaculture. 2022, 557 . urn:issn:0044-8486 https://hdl.handle.net/11250/3015763 https://doi.org/10.1016/j.aquaculture.2022.738318 cristin:2046953 |
op_rights |
Navngivelse-DelPåSammeVilkår 4.0 Internasjonal http://creativecommons.org/licenses/by-sa/4.0/deed.no |
op_rightsnorm |
CC-BY-SA |
op_doi |
https://doi.org/10.1016/j.aquaculture.2022.738318 |
container_title |
Aquaculture |
container_volume |
557 |
container_start_page |
738318 |
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1766361968586784768 |