A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar)
The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respect...
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Online Access: | https://hdl.handle.net/11250/3014633 https://doi.org/10.3390/metabo12020159 |
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ftimr:oai:imr.brage.unit.no:11250/3014633 2023-05-15T15:31:32+02:00 A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) Hundal, Bjørg Kristine Lutfi Royo, Esmail Sigholt, Trygve Rosenlund, Grethe Liland, Nina Sylvia Glencross, Brett Sissener, Nini 2022 application/pdf https://hdl.handle.net/11250/3014633 https://doi.org/10.3390/metabo12020159 eng eng Norges forskningsråd: 273215 Fiskeri- og havbruksnæringens forskningsfinansiering: 901282 Metabolites. 2022, 12 (2), . urn:issn:2218-1989 https://hdl.handle.net/11250/3014633 https://doi.org/10.3390/metabo12020159 cristin:2026318 26 12 Metabolites 2 Peer reviewed Journal article 2022 ftimr https://doi.org/10.3390/metabo12020159 2022-09-07T22:41:47Z The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respectively) were fed in triplicate through a full production cycle from an average starting weight of 275 g to slaughter size (~5 kg). Feeding low dietary EPA + DHA altered the hepatic energy metabolism, evidenced by reductions in tricarboxylic acid cycle intermediates originating from β-oxidation, which was compensated by elevated activity in alternative energy pathways (pentose phosphate pathway, branched chain amino acid catabolism and creatine metabolism). Increases in various acylcarnitines in the liver supported this and indicates issues with lipid metabolism (mitochondrial β-oxidation). Problems using lipids for energy in the lower EPA + DHA groups line up well with observed increases in liver lipids in these fish. It also aligns with the growth data, where fish fed the highest EPA + DHA grew better than the other groups. The study showed that diets 1.0 and 1.3 were insufficient for maintaining good liver metabolic health. However, diet 3.5 was significantly better than diet 1.6, indicating that diet 1.6 might also be suboptimal. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Metabolites 12 2 159 |
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Open Polar |
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Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
The present study aimed at elucidating the effects of graded levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the hepatic metabolic health of Atlantic salmon reared in sea cages. Diets containing 10, 13, 16 and 35 g/kg EPA + DHA (designated diets 1.0, 1.3, 1.6 and 3.5, respectively) were fed in triplicate through a full production cycle from an average starting weight of 275 g to slaughter size (~5 kg). Feeding low dietary EPA + DHA altered the hepatic energy metabolism, evidenced by reductions in tricarboxylic acid cycle intermediates originating from β-oxidation, which was compensated by elevated activity in alternative energy pathways (pentose phosphate pathway, branched chain amino acid catabolism and creatine metabolism). Increases in various acylcarnitines in the liver supported this and indicates issues with lipid metabolism (mitochondrial β-oxidation). Problems using lipids for energy in the lower EPA + DHA groups line up well with observed increases in liver lipids in these fish. It also aligns with the growth data, where fish fed the highest EPA + DHA grew better than the other groups. The study showed that diets 1.0 and 1.3 were insufficient for maintaining good liver metabolic health. However, diet 3.5 was significantly better than diet 1.6, indicating that diet 1.6 might also be suboptimal. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Hundal, Bjørg Kristine Lutfi Royo, Esmail Sigholt, Trygve Rosenlund, Grethe Liland, Nina Sylvia Glencross, Brett Sissener, Nini |
spellingShingle |
Hundal, Bjørg Kristine Lutfi Royo, Esmail Sigholt, Trygve Rosenlund, Grethe Liland, Nina Sylvia Glencross, Brett Sissener, Nini A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) |
author_facet |
Hundal, Bjørg Kristine Lutfi Royo, Esmail Sigholt, Trygve Rosenlund, Grethe Liland, Nina Sylvia Glencross, Brett Sissener, Nini |
author_sort |
Hundal, Bjørg Kristine |
title |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) |
title_short |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) |
title_full |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) |
title_fullStr |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
A Piece of the Puzzle—Possible Mechanisms for Why Low Dietary EPA and DHA Cause Hepatic Lipid Accumulation in Atlantic Salmon (Salmo salar) |
title_sort |
piece of the puzzle—possible mechanisms for why low dietary epa and dha cause hepatic lipid accumulation in atlantic salmon (salmo salar) |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3014633 https://doi.org/10.3390/metabo12020159 |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Slaughter |
geographic_facet |
Slaughter |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
26 12 Metabolites 2 |
op_relation |
Norges forskningsråd: 273215 Fiskeri- og havbruksnæringens forskningsfinansiering: 901282 Metabolites. 2022, 12 (2), . urn:issn:2218-1989 https://hdl.handle.net/11250/3014633 https://doi.org/10.3390/metabo12020159 cristin:2026318 |
op_doi |
https://doi.org/10.3390/metabo12020159 |
container_title |
Metabolites |
container_volume |
12 |
container_issue |
2 |
container_start_page |
159 |
_version_ |
1766362066755518464 |