Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry”
The article ‘No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry’ (Choquet et al. 2020) concludes that “no evidence supports a potential for hybridization between C. finmarchicus and C. glacialis”. We argue that the InDel markers used by Choque...
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Online Access: | https://hdl.handle.net/11250/2983520 https://doi.org/10.1002/lno.11725 |
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ftimr:oai:imr.brage.unit.no:11250/2983520 2023-05-15T15:47:55+02:00 Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” Parent, Geneviève J. Plourde, Stéphane Browman, Howard Turgeon, Julie Petrusek, Adam 2021 application/pdf https://hdl.handle.net/11250/2983520 https://doi.org/10.1002/lno.11725 eng eng Limnology and Oceanography. 2021, 66 (10), 3597-3602. urn:issn:0024-3590 https://hdl.handle.net/11250/2983520 https://doi.org/10.1002/lno.11725 cristin:2000394 3597-3602 66 Limnology and Oceanography 10 Peer reviewed Journal article 2021 ftimr https://doi.org/10.1002/lno.11725 2022-03-09T23:39:25Z The article ‘No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry’ (Choquet et al. 2020) concludes that “no evidence supports a potential for hybridization between C. finmarchicus and C. glacialis”. We argue that the InDel markers used by Choquet et al. (2020) may have limited capacity to detect admixed genotypes between C. finmarchicus and C. glacialis due to an inappropriate choice of reference sample for each species during the marker development. We first review terms and concepts used in genetic classification using reference samples and describe problems associated with the selection of genetic markers in the context of possible hybridization. We reanalyze InDel genotypes provided with Choquet et al. (2017) and identified an admixed individual. We then contrast methods used by Choquet et al. (2017) and Parent et al. (2012) and explain how Parent et al. (2012) developed microsatellite markers capable of discriminating admixed genotypes from parental species. In this comment, we have identified a major issue that must be considered when selecting reference samples in the context of testing for possible hybridization. publishedVersion Article in Journal/Newspaper Calanus finmarchicus Subarctic Institute for Marine Research: Brage IMR Indel’ ENVELOPE(35.282,35.282,66.963,66.963) Limnology and Oceanography 66 10 3597 3602 |
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Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
The article ‘No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry’ (Choquet et al. 2020) concludes that “no evidence supports a potential for hybridization between C. finmarchicus and C. glacialis”. We argue that the InDel markers used by Choquet et al. (2020) may have limited capacity to detect admixed genotypes between C. finmarchicus and C. glacialis due to an inappropriate choice of reference sample for each species during the marker development. We first review terms and concepts used in genetic classification using reference samples and describe problems associated with the selection of genetic markers in the context of possible hybridization. We reanalyze InDel genotypes provided with Choquet et al. (2017) and identified an admixed individual. We then contrast methods used by Choquet et al. (2017) and Parent et al. (2012) and explain how Parent et al. (2012) developed microsatellite markers capable of discriminating admixed genotypes from parental species. In this comment, we have identified a major issue that must be considered when selecting reference samples in the context of testing for possible hybridization. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Parent, Geneviève J. Plourde, Stéphane Browman, Howard Turgeon, Julie Petrusek, Adam |
spellingShingle |
Parent, Geneviève J. Plourde, Stéphane Browman, Howard Turgeon, Julie Petrusek, Adam Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” |
author_facet |
Parent, Geneviève J. Plourde, Stéphane Browman, Howard Turgeon, Julie Petrusek, Adam |
author_sort |
Parent, Geneviève J. |
title |
Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” |
title_short |
Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” |
title_full |
Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” |
title_fullStr |
Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” |
title_full_unstemmed |
Defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: Comment on Choquet et al. 2020 “No evidence for hybridization between Calanus finmarchicus and C. glacialis in a subarctic area of sympatry” |
title_sort |
defining what constitutes a reliable dataset to test for hybridization and introgression in marine zooplankton: comment on choquet et al. 2020 “no evidence for hybridization between calanus finmarchicus and c. glacialis in a subarctic area of sympatry” |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2983520 https://doi.org/10.1002/lno.11725 |
long_lat |
ENVELOPE(35.282,35.282,66.963,66.963) |
geographic |
Indel’ |
geographic_facet |
Indel’ |
genre |
Calanus finmarchicus Subarctic |
genre_facet |
Calanus finmarchicus Subarctic |
op_source |
3597-3602 66 Limnology and Oceanography 10 |
op_relation |
Limnology and Oceanography. 2021, 66 (10), 3597-3602. urn:issn:0024-3590 https://hdl.handle.net/11250/2983520 https://doi.org/10.1002/lno.11725 cristin:2000394 |
op_doi |
https://doi.org/10.1002/lno.11725 |
container_title |
Limnology and Oceanography |
container_volume |
66 |
container_issue |
10 |
container_start_page |
3597 |
op_container_end_page |
3602 |
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1766382885913231360 |