Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH)
Parvicapsula pseudobranchicola is a marine myxosporean parasite infecting farmed Atlantic salmon (Salmo salar). A major site for the parasite is the pseudobranch, which may be destroyed in heavily infected fish. Parvicapsulosis may be associated with significant mortality, although the main effect o...
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BioMed Central
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Online Access: | http://hdl.handle.net/11250/281496 https://doi.org/10.1186/s13071-015-0718-4 |
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ftimr:oai:imr.brage.unit.no:11250/281496 2023-05-15T15:30:59+02:00 Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) Markussen, Turhan Agusti, Celia Karlsbakk, Egil Nylund, Are Brevik, Øyvind Hansen, Haakon 2015-02-15 application/pdf http://hdl.handle.net/11250/281496 https://doi.org/10.1186/s13071-015-0718-4 eng eng BioMed Central Markussen, T., Agusti, C., Karlsbakk, E., Nylund, A., Brevik, O., Hansen, H., 2015. Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH). Parasites & Vectors. 8, 105. urn:issn:1756-3305 http://hdl.handle.net/11250/281496 https://doi.org/10.1186/s13071-015-0718-4 Navngivelse 3.0 Norge http://creativecommons.org/licenses/by/3.0/no/ CC-BY 6 s. 8 Parasites & Vectors VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Journal article Peer reviewed 2015 ftimr https://doi.org/10.1186/s13071-015-0718-4 2021-09-23T20:14:41Z Parvicapsula pseudobranchicola is a marine myxosporean parasite infecting farmed Atlantic salmon (Salmo salar). A major site for the parasite is the pseudobranch, which may be destroyed in heavily infected fish. Parvicapsulosis may be associated with significant mortality, although the main effect of infections seems to be runting. In situ hybridization (ISH) is, in the absence of specific antibodies, the preferred method for the detection of cell- and tissue tropisms of myxozoans in the early phases of infection of the host, and provides information about the possible association between the pathogen and pathology. A positive diagnosis of parvicapsulosis is based on histopathology and PCR. The aim of the present work was to develop a specific, sensitive and robust ISH assay for the detection of P. pseudobranchicola in tissues. Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Parasites & Vectors 8 1 105 |
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Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
topic |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 |
spellingShingle |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 Markussen, Turhan Agusti, Celia Karlsbakk, Egil Nylund, Are Brevik, Øyvind Hansen, Haakon Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) |
topic_facet |
VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 |
description |
Parvicapsula pseudobranchicola is a marine myxosporean parasite infecting farmed Atlantic salmon (Salmo salar). A major site for the parasite is the pseudobranch, which may be destroyed in heavily infected fish. Parvicapsulosis may be associated with significant mortality, although the main effect of infections seems to be runting. In situ hybridization (ISH) is, in the absence of specific antibodies, the preferred method for the detection of cell- and tissue tropisms of myxozoans in the early phases of infection of the host, and provides information about the possible association between the pathogen and pathology. A positive diagnosis of parvicapsulosis is based on histopathology and PCR. The aim of the present work was to develop a specific, sensitive and robust ISH assay for the detection of P. pseudobranchicola in tissues. |
format |
Article in Journal/Newspaper |
author |
Markussen, Turhan Agusti, Celia Karlsbakk, Egil Nylund, Are Brevik, Øyvind Hansen, Haakon |
author_facet |
Markussen, Turhan Agusti, Celia Karlsbakk, Egil Nylund, Are Brevik, Øyvind Hansen, Haakon |
author_sort |
Markussen, Turhan |
title |
Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) |
title_short |
Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) |
title_full |
Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) |
title_fullStr |
Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) |
title_full_unstemmed |
Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH) |
title_sort |
detection of the myxosporean parasite parvicapsula pseudobranchicola in atlantic salmon (salmo salar l.) using in situ hybridization (ish) |
publisher |
BioMed Central |
publishDate |
2015 |
url |
http://hdl.handle.net/11250/281496 https://doi.org/10.1186/s13071-015-0718-4 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
6 s. 8 Parasites & Vectors |
op_relation |
Markussen, T., Agusti, C., Karlsbakk, E., Nylund, A., Brevik, O., Hansen, H., 2015. Detection of the myxosporean parasite Parvicapsula pseudobranchicola in Atlantic salmon (Salmo salar L.) using in situ hybridization (ISH). Parasites & Vectors. 8, 105. urn:issn:1756-3305 http://hdl.handle.net/11250/281496 https://doi.org/10.1186/s13071-015-0718-4 |
op_rights |
Navngivelse 3.0 Norge http://creativecommons.org/licenses/by/3.0/no/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1186/s13071-015-0718-4 |
container_title |
Parasites & Vectors |
container_volume |
8 |
container_issue |
1 |
container_start_page |
105 |
_version_ |
1766361464692539392 |