Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar)
The microbial communities that live in symbiosis with the mucosal surfaces of animals provide the host with defense strategies against pathogens. These microbial communities are largely shaped by the environment and the host genetics. Triploid Atlantic salmon (Salmo salar) are being considered for a...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2021
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/2763772 https://doi.org/10.1371/journal.pone.0243684 |
id |
ftimr:oai:imr.brage.unit.no:11250/2763772 |
---|---|
record_format |
openpolar |
spelling |
ftimr:oai:imr.brage.unit.no:11250/2763772 2023-05-15T15:29:44+02:00 Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) Brown, Ryan Moore, Lindsey Mani, Amir Patel, Sonal Salinas, Irene 2021 application/pdf https://hdl.handle.net/11250/2763772 https://doi.org/10.1371/journal.pone.0243684 eng eng Norges forskningsråd: 224885 PLOS ONE. 2021, 16 (2), 1-17. urn:issn:1932-6203 https://hdl.handle.net/11250/2763772 https://doi.org/10.1371/journal.pone.0243684 cristin:1917743 1-17 16 PLOS ONE 2 Peer reviewed Journal article 2021 ftimr https://doi.org/10.1371/journal.pone.0243684 2021-09-23T20:14:18Z The microbial communities that live in symbiosis with the mucosal surfaces of animals provide the host with defense strategies against pathogens. These microbial communities are largely shaped by the environment and the host genetics. Triploid Atlantic salmon (Salmo salar) are being considered for aquaculture as they are reproductively sterile and thus cannot contaminate the natural gene pool. It has not been previously investigated how the microbiome of triploid salmon compares to that of their diploid counterparts. In this study, we compare the steady-state skin and gill microbiome of both diploid and triploid salmon, and determine the effects of salmonid alphavirus 3 experimental infection on their microbial composition. Our results show limited differences in the skin-associated microbiome between triploid and diploid salmon, irrespective of infection. In the gills, we observed a high incidence of the bacterial pathogen Candidatus Branchiomonas, with higher abundance in diploid compared to triploid control fish. Diploid salmon infected with SAV3 showed greater histopathological signs of epitheliocystis compared to controls, a phenomenon not observed in triploid fish. Our results indicate that ploidy can affect the alpha diversity of the gills but not the skin-associated microbial community. Importantly, during a natural outbreak of Branchiomonas sp. the gill microbiome of diploid Atlantic salmon became significantly more dominated by this pathogen than in triploid animals. Thus, our results suggest that ploidy may play a role on Atlantic salmon gill health and provide insights into co-infection with SAV3 and C. Branchiomonas in Atlantic salmon. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR PLOS ONE 16 2 e0243684 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
The microbial communities that live in symbiosis with the mucosal surfaces of animals provide the host with defense strategies against pathogens. These microbial communities are largely shaped by the environment and the host genetics. Triploid Atlantic salmon (Salmo salar) are being considered for aquaculture as they are reproductively sterile and thus cannot contaminate the natural gene pool. It has not been previously investigated how the microbiome of triploid salmon compares to that of their diploid counterparts. In this study, we compare the steady-state skin and gill microbiome of both diploid and triploid salmon, and determine the effects of salmonid alphavirus 3 experimental infection on their microbial composition. Our results show limited differences in the skin-associated microbiome between triploid and diploid salmon, irrespective of infection. In the gills, we observed a high incidence of the bacterial pathogen Candidatus Branchiomonas, with higher abundance in diploid compared to triploid control fish. Diploid salmon infected with SAV3 showed greater histopathological signs of epitheliocystis compared to controls, a phenomenon not observed in triploid fish. Our results indicate that ploidy can affect the alpha diversity of the gills but not the skin-associated microbial community. Importantly, during a natural outbreak of Branchiomonas sp. the gill microbiome of diploid Atlantic salmon became significantly more dominated by this pathogen than in triploid animals. Thus, our results suggest that ploidy may play a role on Atlantic salmon gill health and provide insights into co-infection with SAV3 and C. Branchiomonas in Atlantic salmon. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Brown, Ryan Moore, Lindsey Mani, Amir Patel, Sonal Salinas, Irene |
spellingShingle |
Brown, Ryan Moore, Lindsey Mani, Amir Patel, Sonal Salinas, Irene Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) |
author_facet |
Brown, Ryan Moore, Lindsey Mani, Amir Patel, Sonal Salinas, Irene |
author_sort |
Brown, Ryan |
title |
Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) |
title_short |
Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) |
title_full |
Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) |
title_fullStr |
Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of Atlantic salmon (Salmo salar) |
title_sort |
effects of ploidy and salmonid alphavirus infection on the skin and gill microbiome of atlantic salmon (salmo salar) |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2763772 https://doi.org/10.1371/journal.pone.0243684 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
1-17 16 PLOS ONE 2 |
op_relation |
Norges forskningsråd: 224885 PLOS ONE. 2021, 16 (2), 1-17. urn:issn:1932-6203 https://hdl.handle.net/11250/2763772 https://doi.org/10.1371/journal.pone.0243684 cristin:1917743 |
op_doi |
https://doi.org/10.1371/journal.pone.0243684 |
container_title |
PLOS ONE |
container_volume |
16 |
container_issue |
2 |
container_start_page |
e0243684 |
_version_ |
1766360181273264128 |