Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring
The Atlantic salmon aquaculture industry relies on adjustments of female broodstock spawning season to meet the demand for delivery of embryos outside the natural spawning season. Earlier results from zebrafish have shown that parental micronutrient status program offspring metabolism. Therefore, th...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Online Access: | https://hdl.handle.net/11250/2728273 https://doi.org/10.1016/j.cbpa.2020.110717 |
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ftimr:oai:imr.brage.unit.no:11250/2728273 2023-05-15T15:31:13+02:00 Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring Skjærven, Kaja Helvik Oveland, Eystein Mommens, Maren Samori, Elsa Saito, Takaya Adam, Anne-Catrin Espe, Marit 2020 application/pdf https://hdl.handle.net/11250/2728273 https://doi.org/10.1016/j.cbpa.2020.110717 eng eng Norges forskningsråd: 267787 Comparative Biochemistry and Physiology A. 2020, 247 . urn:issn:1095-6433 https://hdl.handle.net/11250/2728273 https://doi.org/10.1016/j.cbpa.2020.110717 cristin:1886329 9 247 Comparative Biochemistry and Physiology A 110717 Peer reviewed Journal article 2020 ftimr https://doi.org/10.1016/j.cbpa.2020.110717 2021-09-23T20:15:14Z The Atlantic salmon aquaculture industry relies on adjustments of female broodstock spawning season to meet the demand for delivery of embryos outside the natural spawning season. Earlier results from zebrafish have shown that parental micronutrient status program offspring metabolism. Therefore, the main hypothesis of this study was to investigate if out-of-season (off-season) broodstock (spawning in June, in land-based recirculation systems) and their offspring deviate in micronutrient status when compared to broodstock and offspring from normal spawning season. Both seasons of female Atlantic salmon broodstock were fed the same diet and starved for approximately the same time interval prior to spawning. We compared nutrients related to the 1C metabolism (vitamin B12, folate, vitamin B6, methionine), free amino acids (FAAs) and lipid classes in broodstock muscle and liver tissues, and during offspring ontogeny. In general, the off-season broodstock showed higher levels of folate, vitamin B6 and selected FAAs in muscle tissue, and higher levels of folate and lipids (cholesterol and sphingomyelin) in liver tissue compared to normal-season. Furthermore, embryos from off-season had reduced amounts of all the measured lipid classes, like cholesterol and sphingomyelin, and lower levels of one type of folate and changes in FAAs and N-metabolites. We discovered significant differences between the seasons in mRNA levels of genes controlling fatty acid synthesis and 1C metabolism in both broodstock liver and offspring. Moreover, for genes controlling the methylation of DNA; both maintenance and de novo DNA methyltransferases (DNMTs) were expressed at higher levels in off-season compared to normal-season offspring. Our results show, in general that normal spawning season broodstock allocated more nutrients to eggs than off-season. Our results indicate a potential for improved maturation for off-season group to obtain a higher offspring growth potential, and this argues for a reassessment of the nutritional influence from broodstock to offspring and the consequences through nutritional programming. publishedVersion Article in Journal/Newspaper Atlantic salmon Institute for Marine Research: Brage IMR Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 247 110717 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
The Atlantic salmon aquaculture industry relies on adjustments of female broodstock spawning season to meet the demand for delivery of embryos outside the natural spawning season. Earlier results from zebrafish have shown that parental micronutrient status program offspring metabolism. Therefore, the main hypothesis of this study was to investigate if out-of-season (off-season) broodstock (spawning in June, in land-based recirculation systems) and their offspring deviate in micronutrient status when compared to broodstock and offspring from normal spawning season. Both seasons of female Atlantic salmon broodstock were fed the same diet and starved for approximately the same time interval prior to spawning. We compared nutrients related to the 1C metabolism (vitamin B12, folate, vitamin B6, methionine), free amino acids (FAAs) and lipid classes in broodstock muscle and liver tissues, and during offspring ontogeny. In general, the off-season broodstock showed higher levels of folate, vitamin B6 and selected FAAs in muscle tissue, and higher levels of folate and lipids (cholesterol and sphingomyelin) in liver tissue compared to normal-season. Furthermore, embryos from off-season had reduced amounts of all the measured lipid classes, like cholesterol and sphingomyelin, and lower levels of one type of folate and changes in FAAs and N-metabolites. We discovered significant differences between the seasons in mRNA levels of genes controlling fatty acid synthesis and 1C metabolism in both broodstock liver and offspring. Moreover, for genes controlling the methylation of DNA; both maintenance and de novo DNA methyltransferases (DNMTs) were expressed at higher levels in off-season compared to normal-season offspring. Our results show, in general that normal spawning season broodstock allocated more nutrients to eggs than off-season. Our results indicate a potential for improved maturation for off-season group to obtain a higher offspring growth potential, and this argues for a reassessment of the nutritional influence from broodstock to offspring and the consequences through nutritional programming. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Skjærven, Kaja Helvik Oveland, Eystein Mommens, Maren Samori, Elsa Saito, Takaya Adam, Anne-Catrin Espe, Marit |
spellingShingle |
Skjærven, Kaja Helvik Oveland, Eystein Mommens, Maren Samori, Elsa Saito, Takaya Adam, Anne-Catrin Espe, Marit Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring |
author_facet |
Skjærven, Kaja Helvik Oveland, Eystein Mommens, Maren Samori, Elsa Saito, Takaya Adam, Anne-Catrin Espe, Marit |
author_sort |
Skjærven, Kaja Helvik |
title |
Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring |
title_short |
Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring |
title_full |
Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring |
title_fullStr |
Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring |
title_full_unstemmed |
Out-of-season spawning affects the nutritional status and gene expression in both Atlantic salmon female broodstock and their offspring |
title_sort |
out-of-season spawning affects the nutritional status and gene expression in both atlantic salmon female broodstock and their offspring |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2728273 https://doi.org/10.1016/j.cbpa.2020.110717 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
9 247 Comparative Biochemistry and Physiology A 110717 |
op_relation |
Norges forskningsråd: 267787 Comparative Biochemistry and Physiology A. 2020, 247 . urn:issn:1095-6433 https://hdl.handle.net/11250/2728273 https://doi.org/10.1016/j.cbpa.2020.110717 cristin:1886329 |
op_doi |
https://doi.org/10.1016/j.cbpa.2020.110717 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
247 |
container_start_page |
110717 |
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1766361712442736640 |