Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa
Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorp...
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Online Access: | https://hdl.handle.net/11250/2682647 https://doi.org/10.3390/ani10081374 |
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ftimr:oai:imr.brage.unit.no:11250/2682647 2023-05-15T15:31:23+02:00 Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa Tartor, Haitham Monjane, Adérito Luis Grove, Søren 2020 application/pdf https://hdl.handle.net/11250/2682647 https://doi.org/10.3390/ani10081374 eng eng Louse-off 1 Norges forskningsråd: 235477 urn:issn:2076-2615 https://hdl.handle.net/11250/2682647 https://doi.org/10.3390/ani10081374 cristin:1821464 10 Animals 8 Peer reviewed Journal article 2020 ftimr https://doi.org/10.3390/ani10081374 2021-09-23T20:15:01Z Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorption method was the least invasive. We also compared the abundance of antigen-specific immunoglobulin M subtype A antibodies (IgM-A Ab) and complement component 5 (C5) in mucus samples collected from vaccinated fish by the three methods. An enzyme-cascade-amplification strategy colorimetric immune assay was optimized and used to analyze IgM-A, and ELISA was used to analyze C5. The abundance of antigen-specific IgM-A in skin mucus was comparable between the three methods, but C5 was significantly lower in absorbed mucus in comparison to in the wiped or scraped mucus samples. Absorbed skin mucus samples collected from various body regions of salmon, levels of C5 were comparable, while specific IgM-A amounts varied between the regions. By comparing three mucus-absorbing materials (medical wipe, gauze, and cotton) for their ability to absorb and release IgM-A and C5, medical wipes proved to be ideal for IgM-A analysis, whereas gauze was the best for C5 analysis. publishedVersion Article in Journal/Newspaper Atlantic salmon Institute for Marine Research: Brage IMR Animals 10 8 1374 |
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Open Polar |
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Institute for Marine Research: Brage IMR |
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English |
description |
Protocols used to collect fish skin mucus may inadvertently compromise the sampled fish or the resulting sample. Here, we used three methods (wiping, scraping, and absorption) to collect skin mucus from Atlantic salmon and compared their invasiveness on fish skin epithelium. We found that the absorption method was the least invasive. We also compared the abundance of antigen-specific immunoglobulin M subtype A antibodies (IgM-A Ab) and complement component 5 (C5) in mucus samples collected from vaccinated fish by the three methods. An enzyme-cascade-amplification strategy colorimetric immune assay was optimized and used to analyze IgM-A, and ELISA was used to analyze C5. The abundance of antigen-specific IgM-A in skin mucus was comparable between the three methods, but C5 was significantly lower in absorbed mucus in comparison to in the wiped or scraped mucus samples. Absorbed skin mucus samples collected from various body regions of salmon, levels of C5 were comparable, while specific IgM-A amounts varied between the regions. By comparing three mucus-absorbing materials (medical wipe, gauze, and cotton) for their ability to absorb and release IgM-A and C5, medical wipes proved to be ideal for IgM-A analysis, whereas gauze was the best for C5 analysis. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Tartor, Haitham Monjane, Adérito Luis Grove, Søren |
spellingShingle |
Tartor, Haitham Monjane, Adérito Luis Grove, Søren Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
author_facet |
Tartor, Haitham Monjane, Adérito Luis Grove, Søren |
author_sort |
Tartor, Haitham |
title |
Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
title_short |
Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
title_full |
Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
title_fullStr |
Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
title_full_unstemmed |
Quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
title_sort |
quantification of defensive proteins in skin mucus of atlantic salmon using minimally invasive sampling and high-sensitivity elisa |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2682647 https://doi.org/10.3390/ani10081374 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
10 Animals 8 |
op_relation |
Louse-off 1 Norges forskningsråd: 235477 urn:issn:2076-2615 https://hdl.handle.net/11250/2682647 https://doi.org/10.3390/ani10081374 cristin:1821464 |
op_doi |
https://doi.org/10.3390/ani10081374 |
container_title |
Animals |
container_volume |
10 |
container_issue |
8 |
container_start_page |
1374 |
_version_ |
1766361886004084736 |