Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin
The following study aimed to develop a fatty liver model in primary hepatocytes isolated from Atlantic salmon. In order to induce the fatty liver, oleic acid (OA) at 0.2 or 0.4 mM was used. Metformin, known to prevent and cure fatty liver in mammalian cells, was used at 1 or 10 mM for 24 hr before a...
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Language: | English |
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Online Access: | http://hdl.handle.net/11250/2639987 https://doi.org/10.1111/anu.12905 |
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ftimr:oai:imr.brage.unit.no:11250/2639987 2023-05-15T15:31:38+02:00 Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin Espe, Marit Xie, Shiwei Chen, Shijun Araujo, Pedro Holen, Elisabeth 2019 application/pdf http://hdl.handle.net/11250/2639987 https://doi.org/10.1111/anu.12905 eng eng Aquaculture Nutrition. 2019, 25 (3), 737-746. urn:issn:1353-5773 http://hdl.handle.net/11250/2639987 https://doi.org/10.1111/anu.12905 cristin:1702591 737-746 25 Aquaculture Nutrition 3 Journal article Peer reviewed 2019 ftimr https://doi.org/10.1111/anu.12905 2021-09-23T20:14:37Z The following study aimed to develop a fatty liver model in primary hepatocytes isolated from Atlantic salmon. In order to induce the fatty liver, oleic acid (OA) at 0.2 or 0.4 mM was used. Metformin, known to prevent and cure fatty liver in mammalian cells, was used at 1 or 10 mM for 24 hr before addition of OA to test possible prevention effect of metformin on the OA‐induced fatty liver phenotype. Cells grown in 0.2 mM OA did not increase the mean number of lipid droplets, while cells grown in 0.4 mM OA increased the number of lipid droplets within the liver cells (p < 0.0001). Metformin pretreatment prior to OA supplementation reduced the mean number of lipid droplets. Gene expression of ApoB100, CD36 and PPARa increased in cells treated with metformin and most so at 10 mM. On the other hand, gene expression of LXR, SREBP2 and CPT‐1 decreased at both concentrations of metformin, while OA treatment did not affect these genes. Gene expression of IL‐8 increased by 0.4 mM OA (p = 0.002). Metformin reduced the gene expression of IL‐8. Thus, metformin efficiently enhanced the expression of genes related to transport and oxidation of lipids in hepatic cells of salmon, but required higher concentrations of OA and metformin than those required in rodent models to increase and prevent lipid accumulation, respectively. publishedVersion Article in Journal/Newspaper Atlantic salmon Institute for Marine Research: Brage IMR Aquaculture Nutrition 25 3 737 746 |
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Open Polar |
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Institute for Marine Research: Brage IMR |
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English |
description |
The following study aimed to develop a fatty liver model in primary hepatocytes isolated from Atlantic salmon. In order to induce the fatty liver, oleic acid (OA) at 0.2 or 0.4 mM was used. Metformin, known to prevent and cure fatty liver in mammalian cells, was used at 1 or 10 mM for 24 hr before addition of OA to test possible prevention effect of metformin on the OA‐induced fatty liver phenotype. Cells grown in 0.2 mM OA did not increase the mean number of lipid droplets, while cells grown in 0.4 mM OA increased the number of lipid droplets within the liver cells (p < 0.0001). Metformin pretreatment prior to OA supplementation reduced the mean number of lipid droplets. Gene expression of ApoB100, CD36 and PPARa increased in cells treated with metformin and most so at 10 mM. On the other hand, gene expression of LXR, SREBP2 and CPT‐1 decreased at both concentrations of metformin, while OA treatment did not affect these genes. Gene expression of IL‐8 increased by 0.4 mM OA (p = 0.002). Metformin reduced the gene expression of IL‐8. Thus, metformin efficiently enhanced the expression of genes related to transport and oxidation of lipids in hepatic cells of salmon, but required higher concentrations of OA and metformin than those required in rodent models to increase and prevent lipid accumulation, respectively. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Espe, Marit Xie, Shiwei Chen, Shijun Araujo, Pedro Holen, Elisabeth |
spellingShingle |
Espe, Marit Xie, Shiwei Chen, Shijun Araujo, Pedro Holen, Elisabeth Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin |
author_facet |
Espe, Marit Xie, Shiwei Chen, Shijun Araujo, Pedro Holen, Elisabeth |
author_sort |
Espe, Marit |
title |
Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin |
title_short |
Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin |
title_full |
Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin |
title_fullStr |
Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin |
title_full_unstemmed |
Development of a fatty liver model using oleic acid in primary liver cells isolated from Atlantic salmon and the prevention of lipid accumulation using metformin |
title_sort |
development of a fatty liver model using oleic acid in primary liver cells isolated from atlantic salmon and the prevention of lipid accumulation using metformin |
publishDate |
2019 |
url |
http://hdl.handle.net/11250/2639987 https://doi.org/10.1111/anu.12905 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
737-746 25 Aquaculture Nutrition 3 |
op_relation |
Aquaculture Nutrition. 2019, 25 (3), 737-746. urn:issn:1353-5773 http://hdl.handle.net/11250/2639987 https://doi.org/10.1111/anu.12905 cristin:1702591 |
op_doi |
https://doi.org/10.1111/anu.12905 |
container_title |
Aquaculture Nutrition |
container_volume |
25 |
container_issue |
3 |
container_start_page |
737 |
op_container_end_page |
746 |
_version_ |
1766362169415303168 |