Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration
Farming of lumpfish for biological removal of sea lice from farmed Atlantic salmon has expanded rapidly in Europe and Canada over the last 5–6 years and the lumpfish has become an economically important species. There are, however, health challenges associated with bacterial diseases. In recent year...
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Online Access: | http://hdl.handle.net/11250/2611597 https://doi.org/10.1016/j.aquaculture.2019.734279 |
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ftimr:oai:imr.brage.unit.no:11250/2611597 2024-09-15T17:56:34+00:00 Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration Kverme, Karen obrestad Haugland, Gyri Teien Hannisdal, Rita Marielle, Kallekleiv Colquhoun, Duncan John Lunestad, Bjørn Tore Wergeland, Heidrun Inger Samuelsen, Ole Bent 2019 application/pdf http://hdl.handle.net/11250/2611597 https://doi.org/10.1016/j.aquaculture.2019.734279 eng eng Fiskeri- og havbruksnæringens forskningsfond: 901468 Norges forskningsråd: 244148 Aquaculture. 2019, 512 . urn:issn:0044-8486 http://hdl.handle.net/11250/2611597 https://doi.org/10.1016/j.aquaculture.2019.734279 cristin:1718542 7 512 Aquaculture Journal article Peer reviewed 2019 ftimr https://doi.org/10.1016/j.aquaculture.2019.734279 2024-07-31T03:37:25Z Farming of lumpfish for biological removal of sea lice from farmed Atlantic salmon has expanded rapidly in Europe and Canada over the last 5–6 years and the lumpfish has become an economically important species. There are, however, health challenges associated with bacterial diseases. In recent years, there has been an increase in antibacterial treatments prescribed for this fish species despite a lack of knowledge regarding pharmacokinetics and effect of treatment with different antibiotics. The present study examined the uptake, tissue distribution, metabolism and elimination of the antibacterial agent florfenicol in lumpfish (Cyclopterus lumpus L.) following a single oral administration of 10 mg/kg fish given in feed. Plasma, head kidney, liver and muscle from six fish were sampled at each time point and analysed by liquid chromatography/mass spectrometry (LC-MS). Absorption was moderate for this drug characterised by a calculated peak plasma concentration (Cmax) of 3.55 μg/ml obtained after 21.2 hours (Tmax) and the elimination halflife (t1/2β) relatively extended in plasma at 30 hours. Area under curve (AUC) and AUC from 0 to 24 hours (AUC0-24h) were calculated to be 248 and 61 h μg/ml, respectively. Cmax was calculated to 2.99 μg/g in muscle, 2.54 μg/g in liver and 4.70 μg/g in head kidney with corresponding Tmax of 22.1, 26.4 and 19.4 h, respectively. The main metabolite, florfenicol-amine was found in low concentrations in plasma and all tissues examined. The minimum inhibition concentrations (MIC) for florfenicol of 28 of Aeromonas salmonicida isolates from diseased lumpfish ranged from 0.39 to 1.56 μg/ml. The pharmacokinetical data presented here make an important basis for efficient antibacterial treatment for lumpfish using florfenicol and for calculation of suitable withdrawal time. Knowledge of florfenicol pharmacokinetics, combined with determination of antibiotic resistance among fish pathogenic bacteria and the effect of antibacterial agents on diseased lumpfish in vivo are important for the ... Article in Journal/Newspaper Atlantic salmon Institute for Marine Research: Brage IMR Aquaculture 512 734279 |
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Open Polar |
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Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
description |
Farming of lumpfish for biological removal of sea lice from farmed Atlantic salmon has expanded rapidly in Europe and Canada over the last 5–6 years and the lumpfish has become an economically important species. There are, however, health challenges associated with bacterial diseases. In recent years, there has been an increase in antibacterial treatments prescribed for this fish species despite a lack of knowledge regarding pharmacokinetics and effect of treatment with different antibiotics. The present study examined the uptake, tissue distribution, metabolism and elimination of the antibacterial agent florfenicol in lumpfish (Cyclopterus lumpus L.) following a single oral administration of 10 mg/kg fish given in feed. Plasma, head kidney, liver and muscle from six fish were sampled at each time point and analysed by liquid chromatography/mass spectrometry (LC-MS). Absorption was moderate for this drug characterised by a calculated peak plasma concentration (Cmax) of 3.55 μg/ml obtained after 21.2 hours (Tmax) and the elimination halflife (t1/2β) relatively extended in plasma at 30 hours. Area under curve (AUC) and AUC from 0 to 24 hours (AUC0-24h) were calculated to be 248 and 61 h μg/ml, respectively. Cmax was calculated to 2.99 μg/g in muscle, 2.54 μg/g in liver and 4.70 μg/g in head kidney with corresponding Tmax of 22.1, 26.4 and 19.4 h, respectively. The main metabolite, florfenicol-amine was found in low concentrations in plasma and all tissues examined. The minimum inhibition concentrations (MIC) for florfenicol of 28 of Aeromonas salmonicida isolates from diseased lumpfish ranged from 0.39 to 1.56 μg/ml. The pharmacokinetical data presented here make an important basis for efficient antibacterial treatment for lumpfish using florfenicol and for calculation of suitable withdrawal time. Knowledge of florfenicol pharmacokinetics, combined with determination of antibiotic resistance among fish pathogenic bacteria and the effect of antibacterial agents on diseased lumpfish in vivo are important for the ... |
format |
Article in Journal/Newspaper |
author |
Kverme, Karen obrestad Haugland, Gyri Teien Hannisdal, Rita Marielle, Kallekleiv Colquhoun, Duncan John Lunestad, Bjørn Tore Wergeland, Heidrun Inger Samuelsen, Ole Bent |
spellingShingle |
Kverme, Karen obrestad Haugland, Gyri Teien Hannisdal, Rita Marielle, Kallekleiv Colquhoun, Duncan John Lunestad, Bjørn Tore Wergeland, Heidrun Inger Samuelsen, Ole Bent Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration |
author_facet |
Kverme, Karen obrestad Haugland, Gyri Teien Hannisdal, Rita Marielle, Kallekleiv Colquhoun, Duncan John Lunestad, Bjørn Tore Wergeland, Heidrun Inger Samuelsen, Ole Bent |
author_sort |
Kverme, Karen obrestad |
title |
Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration |
title_short |
Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration |
title_full |
Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration |
title_fullStr |
Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration |
title_full_unstemmed |
Pharmacokinetics of florfenicol in lumpfish (Cyclopterus lumpus L.) after a single oral administration |
title_sort |
pharmacokinetics of florfenicol in lumpfish (cyclopterus lumpus l.) after a single oral administration |
publishDate |
2019 |
url |
http://hdl.handle.net/11250/2611597 https://doi.org/10.1016/j.aquaculture.2019.734279 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
7 512 Aquaculture |
op_relation |
Fiskeri- og havbruksnæringens forskningsfond: 901468 Norges forskningsråd: 244148 Aquaculture. 2019, 512 . urn:issn:0044-8486 http://hdl.handle.net/11250/2611597 https://doi.org/10.1016/j.aquaculture.2019.734279 cristin:1718542 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2019.734279 |
container_title |
Aquaculture |
container_volume |
512 |
container_start_page |
734279 |
_version_ |
1810432767597477888 |