Estimating cod egg developmental stage based on DNA concentration
Pelagic egg surveys are commonly used to map Atlantic cod spawning areas in the northeastern Atlantic. However, a sampling location may not necessarily indicate a spawning location, because more developed eggs may have drifted long distances in coastal currents. Newly spawned eggs have only a few em...
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Online Access: | http://hdl.handle.net/11250/2484936 https://doi.org/10.1093/icesjms/fsx172 |
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ftimr:oai:imr.brage.unit.no:11250/2484936 2023-05-15T15:27:11+02:00 Estimating cod egg developmental stage based on DNA concentration Espeland, Sigurd Heiberg Sannæs, Hanne 2017 application/pdf http://hdl.handle.net/11250/2484936 https://doi.org/10.1093/icesjms/fsx172 eng eng ICES Journal of Marine Science. 2017, . urn:issn:1054-3139 http://hdl.handle.net/11250/2484936 https://doi.org/10.1093/icesjms/fsx172 cristin:1565020 6 ICES Journal of Marine Science Journal article Peer reviewed 2017 ftimr https://doi.org/10.1093/icesjms/fsx172 2021-09-23T20:14:21Z Pelagic egg surveys are commonly used to map Atlantic cod spawning areas in the northeastern Atlantic. However, a sampling location may not necessarily indicate a spawning location, because more developed eggs may have drifted long distances in coastal currents. Newly spawned eggs have only a few embryonic cells, whereas eggs in later developmental stages have progressively larger numbers of cells and hence greater amounts of DNA. The progression through developmental stages largely depends on temperature, which influences cell division and growth. Preservation of the eggs for later analysis presents logistical dilemmas. Preservation in ethanol is suitable for DNA extraction but obscures developmental stage, as the eggs shrink and turn opaque. On the other hand, preservation in formaldehyde retains visual characteristics, but this method limits the ability to extract intact DNA. Here we report a method to estimate developmental stages in Atlantic cod eggs by assaying the amount of DNA from eggs preserved in ethanol. We used a correlation between the amount of DNA in an egg and its embryonic developmental stage in samples from western Norway and used this correlation to estimate developmental stages of eggs sampled in northern Norway. publishedVersion Article in Journal/Newspaper atlantic cod Northern Norway Institute for Marine Research: Brage IMR Norway ICES Journal of Marine Science 75 2 825 830 |
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Open Polar |
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Institute for Marine Research: Brage IMR |
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ftimr |
language |
English |
description |
Pelagic egg surveys are commonly used to map Atlantic cod spawning areas in the northeastern Atlantic. However, a sampling location may not necessarily indicate a spawning location, because more developed eggs may have drifted long distances in coastal currents. Newly spawned eggs have only a few embryonic cells, whereas eggs in later developmental stages have progressively larger numbers of cells and hence greater amounts of DNA. The progression through developmental stages largely depends on temperature, which influences cell division and growth. Preservation of the eggs for later analysis presents logistical dilemmas. Preservation in ethanol is suitable for DNA extraction but obscures developmental stage, as the eggs shrink and turn opaque. On the other hand, preservation in formaldehyde retains visual characteristics, but this method limits the ability to extract intact DNA. Here we report a method to estimate developmental stages in Atlantic cod eggs by assaying the amount of DNA from eggs preserved in ethanol. We used a correlation between the amount of DNA in an egg and its embryonic developmental stage in samples from western Norway and used this correlation to estimate developmental stages of eggs sampled in northern Norway. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Espeland, Sigurd Heiberg Sannæs, Hanne |
spellingShingle |
Espeland, Sigurd Heiberg Sannæs, Hanne Estimating cod egg developmental stage based on DNA concentration |
author_facet |
Espeland, Sigurd Heiberg Sannæs, Hanne |
author_sort |
Espeland, Sigurd Heiberg |
title |
Estimating cod egg developmental stage based on DNA concentration |
title_short |
Estimating cod egg developmental stage based on DNA concentration |
title_full |
Estimating cod egg developmental stage based on DNA concentration |
title_fullStr |
Estimating cod egg developmental stage based on DNA concentration |
title_full_unstemmed |
Estimating cod egg developmental stage based on DNA concentration |
title_sort |
estimating cod egg developmental stage based on dna concentration |
publishDate |
2017 |
url |
http://hdl.handle.net/11250/2484936 https://doi.org/10.1093/icesjms/fsx172 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
atlantic cod Northern Norway |
genre_facet |
atlantic cod Northern Norway |
op_source |
6 ICES Journal of Marine Science |
op_relation |
ICES Journal of Marine Science. 2017, . urn:issn:1054-3139 http://hdl.handle.net/11250/2484936 https://doi.org/10.1093/icesjms/fsx172 cristin:1565020 |
op_doi |
https://doi.org/10.1093/icesjms/fsx172 |
container_title |
ICES Journal of Marine Science |
container_volume |
75 |
container_issue |
2 |
container_start_page |
825 |
op_container_end_page |
830 |
_version_ |
1766357630339514368 |