Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar
Coldwater-associated ulcers, i.e. winter ulcers, in seawater-reared Atlantic salmon Salmo salar L. have been reported in Norway since the late 1980s, and Moritella viscosa has been established as an important factor in the pathogenesis of this condition. As routine histopathological examination of w...
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Online Access: | http://hdl.handle.net/11250/117158 https://doi.org/10.3354/dao02324 |
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ftimr:oai:imr.brage.unit.no:11250/117158 2023-05-15T15:31:04+02:00 Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar Olsen, Anne Berit Nilsen, Helene Sandlund, Nina Mikkelsen, Helene Sørum, H. Colquhoun, D.J. 2011-05-09 application/pdf http://hdl.handle.net/11250/117158 https://doi.org/10.3354/dao02324 eng eng Inter-Research http://hdl.handle.net/11250/117158 http://dx.doi.org/10.3354/dao02324 189-199 94 Diseases of Aquatic Organisms 3 halibut atlantic salmon cod torsk atlantisk laks kveite VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Journal article Peer reviewed 2011 ftimr https://doi.org/10.3354/dao02324 2021-09-23T20:15:28Z Coldwater-associated ulcers, i.e. winter ulcers, in seawater-reared Atlantic salmon Salmo salar L. have been reported in Norway since the late 1980s, and Moritella viscosa has been established as an important factor in the pathogenesis of this condition. As routine histopathological examination of winter ulcer cases in our laboratory revealed frequent presence in ulcers of long, slender rods clearly different from M. viscosa, a closer study focusing on these bacteria was conducted. Field cases of winter ulcers during 2 sampling periods, 1996 and 2004–2005, were investigated and long, slender rods were observed by histopathological examination in 70 and 62.5% of the ulcers examined, respectively, whereas cultivation on marine agar resulted in the isolation of yellowpigmented colonies with long rods from 3 and 13% of the ulcers only. The isolates could be separated into 2 groups, both identified as belonging to the genus Tenacibaculum based on phenotypic characterization and 16S rRNA sequencing. Bath challenge for 7 h confirmed the ability of Group 1 bacterium to produce skin and cornea ulcers. In fish already suffering from M. viscosa-induced ulcers, co-infection with the Group 1 bacterium was established within 1 h. Ulcers from field cases of winter ulcers and from the transmission experiments tested positive by immunohistochemistry with polyclonal antiserum against the Group 1 bacterium but not the Group 2 bacterium. Our results strongly indicate the importance of the Group 1 bacterium in the pathogenesis of winter ulcers in Norway. The bacterium is difficult to isolate and is therefore likely to be underdiagnosed based on cultivation only. 2016-05-09 Article in Journal/Newspaper Atlantic salmon Salmo salar Institute for Marine Research: Brage IMR Norway Diseases of Aquatic Organisms 94 3 189 199 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
topic |
halibut atlantic salmon cod torsk atlantisk laks kveite VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 |
spellingShingle |
halibut atlantic salmon cod torsk atlantisk laks kveite VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Olsen, Anne Berit Nilsen, Helene Sandlund, Nina Mikkelsen, Helene Sørum, H. Colquhoun, D.J. Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar |
topic_facet |
halibut atlantic salmon cod torsk atlantisk laks kveite VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 |
description |
Coldwater-associated ulcers, i.e. winter ulcers, in seawater-reared Atlantic salmon Salmo salar L. have been reported in Norway since the late 1980s, and Moritella viscosa has been established as an important factor in the pathogenesis of this condition. As routine histopathological examination of winter ulcer cases in our laboratory revealed frequent presence in ulcers of long, slender rods clearly different from M. viscosa, a closer study focusing on these bacteria was conducted. Field cases of winter ulcers during 2 sampling periods, 1996 and 2004–2005, were investigated and long, slender rods were observed by histopathological examination in 70 and 62.5% of the ulcers examined, respectively, whereas cultivation on marine agar resulted in the isolation of yellowpigmented colonies with long rods from 3 and 13% of the ulcers only. The isolates could be separated into 2 groups, both identified as belonging to the genus Tenacibaculum based on phenotypic characterization and 16S rRNA sequencing. Bath challenge for 7 h confirmed the ability of Group 1 bacterium to produce skin and cornea ulcers. In fish already suffering from M. viscosa-induced ulcers, co-infection with the Group 1 bacterium was established within 1 h. Ulcers from field cases of winter ulcers and from the transmission experiments tested positive by immunohistochemistry with polyclonal antiserum against the Group 1 bacterium but not the Group 2 bacterium. Our results strongly indicate the importance of the Group 1 bacterium in the pathogenesis of winter ulcers in Norway. The bacterium is difficult to isolate and is therefore likely to be underdiagnosed based on cultivation only. 2016-05-09 |
format |
Article in Journal/Newspaper |
author |
Olsen, Anne Berit Nilsen, Helene Sandlund, Nina Mikkelsen, Helene Sørum, H. Colquhoun, D.J. |
author_facet |
Olsen, Anne Berit Nilsen, Helene Sandlund, Nina Mikkelsen, Helene Sørum, H. Colquhoun, D.J. |
author_sort |
Olsen, Anne Berit |
title |
Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar |
title_short |
Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar |
title_full |
Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar |
title_fullStr |
Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar |
title_full_unstemmed |
Tenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salar |
title_sort |
tenacibaculum sp. associated with winter ulcers in sea-reared atlantic salmon salmo salar |
publisher |
Inter-Research |
publishDate |
2011 |
url |
http://hdl.handle.net/11250/117158 https://doi.org/10.3354/dao02324 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
189-199 94 Diseases of Aquatic Organisms 3 |
op_relation |
http://hdl.handle.net/11250/117158 http://dx.doi.org/10.3354/dao02324 |
op_doi |
https://doi.org/10.3354/dao02324 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
94 |
container_issue |
3 |
container_start_page |
189 |
op_container_end_page |
199 |
_version_ |
1766361552554819584 |