Stereological calibration of the profile method to quickly estimate atresia levels in fish
Journal homepage: http://www.elsevier.com/wps/find/journaldescription.cws_home/503309/description#description The (physical) ‘disector method’, a frequently cited stereological technique, has so far received little attention within fish fecundity studies, although it can be used to provide unbiased,...
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Online Access: | http://hdl.handle.net/11250/117127 https://doi.org/10.1016/j.fishres.2010.03.013 |
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ftimr:oai:imr.brage.unit.no:11250/117127 2023-05-15T15:27:44+02:00 Stereological calibration of the profile method to quickly estimate atresia levels in fish Kjesbu, Olav Sigurd Fonn, Merete Gonzáles, Barbara Dunia Nilsen, Trygve 2010-05 application/pdf http://hdl.handle.net/11250/117127 https://doi.org/10.1016/j.fishres.2010.03.013 eng eng Elsevier urn:issn:0165-7836 http://hdl.handle.net/11250/117127 http://dx.doi.org/10.1016/j.fishres.2010.03.013 8-18 104 Fisheries Research 1-3 fecundity fertilitet VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fisheries technology: 924 Journal article Peer reviewed 2010 ftimr https://doi.org/10.1016/j.fishres.2010.03.013 2021-09-23T20:14:33Z Journal homepage: http://www.elsevier.com/wps/find/journaldescription.cws_home/503309/description#description The (physical) ‘disector method’, a frequently cited stereological technique, has so far received little attention within fish fecundity studies, although it can be used to provide unbiased, assumption-free data on levels of atresia (resorption of vitellogenic oocytes). In comparison, traditional simple counting to estimate the numerical ratio of normal to atretic cells is biased because the smaller atretic cells have a lower chance of being transected in histological sections. These problems are circumvented by the disector method as it operates in three dimensions, i.e., uses consecutive pairs of slides. However, the high labour costs involved prevent regular usage of this method in population studies where large numbers of ovarian samples are processed. In the present paper we assess the corresponding bias of the traditional profile method, analysing developing ovaries of Atlantic cod (Gadus morhua) and herring (Clupea harengus), i.e., in a relatively broad and narrow oocyte frequency situation, respectively. A highly significant but non-linear relationship (r2 = 0.975, P < 0.001, df = 154) was found between the relative intensity of atretic vitellogenic oocytes (ARI) estimated by the disector and profile method. Both species fitted well to this polynomial model. The degree of underestimation of atretic oocytes still containing yolk (i.e., the alpha stage) was at maximum (11.6%) at intermediate levels (ARI = 50% (disector)) but, logically, no such bias existed at the extreme ends (ARI: 0% and 100% (disector)). The practical use of this simple, fast approach designated the stereo-profile method, including the additional use of image analysis for further refinements, is successfully demonstrated on field samples. Article in Journal/Newspaper atlantic cod Gadus morhua Institute for Marine Research: Brage IMR Ari ENVELOPE(147.813,147.813,59.810,59.810) Fisheries Research 104 1-3 8 18 |
institution |
Open Polar |
collection |
Institute for Marine Research: Brage IMR |
op_collection_id |
ftimr |
language |
English |
topic |
fecundity fertilitet VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fisheries technology: 924 |
spellingShingle |
fecundity fertilitet VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fisheries technology: 924 Kjesbu, Olav Sigurd Fonn, Merete Gonzáles, Barbara Dunia Nilsen, Trygve Stereological calibration of the profile method to quickly estimate atresia levels in fish |
topic_facet |
fecundity fertilitet VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fisheries technology: 924 |
description |
Journal homepage: http://www.elsevier.com/wps/find/journaldescription.cws_home/503309/description#description The (physical) ‘disector method’, a frequently cited stereological technique, has so far received little attention within fish fecundity studies, although it can be used to provide unbiased, assumption-free data on levels of atresia (resorption of vitellogenic oocytes). In comparison, traditional simple counting to estimate the numerical ratio of normal to atretic cells is biased because the smaller atretic cells have a lower chance of being transected in histological sections. These problems are circumvented by the disector method as it operates in three dimensions, i.e., uses consecutive pairs of slides. However, the high labour costs involved prevent regular usage of this method in population studies where large numbers of ovarian samples are processed. In the present paper we assess the corresponding bias of the traditional profile method, analysing developing ovaries of Atlantic cod (Gadus morhua) and herring (Clupea harengus), i.e., in a relatively broad and narrow oocyte frequency situation, respectively. A highly significant but non-linear relationship (r2 = 0.975, P < 0.001, df = 154) was found between the relative intensity of atretic vitellogenic oocytes (ARI) estimated by the disector and profile method. Both species fitted well to this polynomial model. The degree of underestimation of atretic oocytes still containing yolk (i.e., the alpha stage) was at maximum (11.6%) at intermediate levels (ARI = 50% (disector)) but, logically, no such bias existed at the extreme ends (ARI: 0% and 100% (disector)). The practical use of this simple, fast approach designated the stereo-profile method, including the additional use of image analysis for further refinements, is successfully demonstrated on field samples. |
format |
Article in Journal/Newspaper |
author |
Kjesbu, Olav Sigurd Fonn, Merete Gonzáles, Barbara Dunia Nilsen, Trygve |
author_facet |
Kjesbu, Olav Sigurd Fonn, Merete Gonzáles, Barbara Dunia Nilsen, Trygve |
author_sort |
Kjesbu, Olav Sigurd |
title |
Stereological calibration of the profile method to quickly estimate atresia levels in fish |
title_short |
Stereological calibration of the profile method to quickly estimate atresia levels in fish |
title_full |
Stereological calibration of the profile method to quickly estimate atresia levels in fish |
title_fullStr |
Stereological calibration of the profile method to quickly estimate atresia levels in fish |
title_full_unstemmed |
Stereological calibration of the profile method to quickly estimate atresia levels in fish |
title_sort |
stereological calibration of the profile method to quickly estimate atresia levels in fish |
publisher |
Elsevier |
publishDate |
2010 |
url |
http://hdl.handle.net/11250/117127 https://doi.org/10.1016/j.fishres.2010.03.013 |
long_lat |
ENVELOPE(147.813,147.813,59.810,59.810) |
geographic |
Ari |
geographic_facet |
Ari |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
8-18 104 Fisheries Research 1-3 |
op_relation |
urn:issn:0165-7836 http://hdl.handle.net/11250/117127 http://dx.doi.org/10.1016/j.fishres.2010.03.013 |
op_doi |
https://doi.org/10.1016/j.fishres.2010.03.013 |
container_title |
Fisheries Research |
container_volume |
104 |
container_issue |
1-3 |
container_start_page |
8 |
op_container_end_page |
18 |
_version_ |
1766358146328035328 |